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KRT6A Recombinant Monoclonal Antibody

  • 中文名稱：
    KRT6A重組抗體
  • 貨號：
    CSB-RA788545A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Immunofluorescence staining of A549 with CSB-RA788545A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 519-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA788545A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA788545A0HU KRT6A重組單克隆抗體是針對角蛋白6A（Keratins 6A）設計的高特異性科研工具。該抗體識別的靶標KRT6A屬于II型中間絲蛋白家族，在表皮分化、細胞機械應力響應中發(fā)揮重要作用，其異常表達與銀屑病、先天性厚甲癥等皮膚疾病相關，同時在多種上皮源性腫瘤中呈現(xiàn)異常調(diào)控。本品經(jīng)ELISA、免疫熒光（IF）和流式細胞術（FC）多平臺嚴格驗證，IF實驗顯示其能清晰標記細胞質內(nèi)角蛋白網(wǎng)絡結構，F(xiàn)C分析驗證其可有效檢測細胞內(nèi)源性KRT6A表達，推薦使用稀釋度為IF 1:50-1:200及FC 1:50-1:200。適用于體外培養(yǎng)細胞的角蛋白表達譜分析、腫瘤上皮間質轉化研究、皮膚病理機制探索等科研領域，也可作為組織切片中角化異常相關疾病的分子探針。該重組抗體采用哺乳動物表達系統(tǒng)生產(chǎn)，具有批次穩(wěn)定性高、交叉反應性低的特點，為上皮生物學及疾病相關研究提供可靠試劑支持。
  • Uniprot No.：
  • 基因名：
    KRT6A
  • 別名：
    Keratin, type II cytoskeletal 6A (Cytokeratin-6A) (CK-6A) (Cytokeratin-6D) (CK-6D) (Keratin-6A) (K6A) (Type-II keratin Kb6) (allergen Hom s 5), KRT6A, K6A KRT6D
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human KRT6A
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    21E2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.
  • 基因功能參考文獻：
    1. Manipulating K6a phosphorylation or ubiquitin-proteasome system (UPS) activity may provide opportunities to harness the innate immunity of epithelia against infection. PMID: 29191848
    2. Several missense polymorphisms in KRT6A, KRT6B and KRT6C that lead to a higher risk for dental caries. PMID: 29357356
    3. KRT6A genetic mutation is associate with the development of Pachyonychia Congenita in patients in Australia. PMID: 27041546
    4. we here describe a family with pachyonychia congenita K6a, manifesting atypical symptoms of impaired wound healing and cheilitis. PMID: 24708461
    5. Keratin-derived antimicrobial peptides (KDAMPs) and their synthetic analogs exhibit antimicrobial activity against bacterial pathogens. PMID: 23006328
    6. Genotype-phenotype correlations among PC patients with codon-125 mutation in KRT16 were established, while the phenotypes caused by the IVS8-2A>C mutation in KRT6A need further studies to confirm the rare feature of fissured tongue PMID: 22668561
    7. We observed a higher likelihood of oral leukokeratosis in individuals harboring KRT6A mutations. PMID: 22264670
    8. Phenotypic differences exist between KRT6A and KRT16 mutations support adoption of a new classification system. PMID: 22098151
    9. This report is the first case of pachyonychia congenita with laryngeal obstruction in which the gene mutation has been established (a deletional mutation in keratin 6a). PMID: 21554383
    10. Focal palmoplantar keratoderma is associated with mutations in keratin K6c in 3 families. 2 unrelated families have Asn172 del and the other has a deletion of AA 462-470. Review. PMID: 20470930
    11. these data highlight the possibility of a physiological role for K6/K16 heterodimers in keratinocyte cell migration, in addition to the heterodimer's known functions in cell differentiation and mechanical resilience. PMID: 20403371
    12. The mutation of 521T--> C in the K6A gene is the causing mutation in pachyonychia congenita type I. PMID: 20140871
    13. Mutations Y465H and N171D of the KRT16A gene were detected in the sporadic pachyonychia congenita cases. PMID: 19806570
    14. Four new missense and five known mutations in K6a, one new deletion and three previously identified missense mutations in K16, plus one known mutation in K17 are reported in pachyonychia congenita. PMID: 17719747
    15. PC-1 is due to mutations of the KRT16 gene or its expression partner KRT6A, wheres PC-2 is caused by mutations in the KRT17 or KRT6B genes. PMID: 18489596
    16. Three novel and four recurrent keratin 6A (KRT6A) mutations were found in Chinese patients with pachyonychia congenita type 1 PMID: 19416275
    17. Rapamycin selectively inhibits expression of an inducible keratin (K6a) in human keratinocytes and improves symptoms in pachyonychia congenita patients. PMID: 19699613

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  • 相關疾?。?/div>
    Pachyonychia congenita 3 (PC3)
  • 蛋白家族：
    Intermediate filament family
  • 組織特異性：
    Expressed in the corneal epithelium (at protein level).
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6443

    OMIM: 148041

    KEGG: hsa:3853

    STRING: 9606.ENSP00000369317

    UniGene: Hs.700779