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LGR6 Recombinant Monoclonal Antibody

  • 中文名稱：
    LGR6重組抗體
  • 貨號(hào)：
    CSB-RA156336A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA156336A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.26% DAB.
    • Immunofluorescence staining of hela with CSB-RA156336A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 500-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells surface stained with CSB-RA156336A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA156336A0HU LGR6重組單克隆抗體是經(jīng)過(guò)嚴(yán)格驗(yàn)證的高特異性科研工具，靶向LGR6（Leucine-rich repeat-containing G-protein coupled receptor 6），該蛋白屬于G蛋白偶聯(lián)受體家族成員，在干細(xì)胞調(diào)控及組織再生中發(fā)揮重要作用，尤其與表皮干細(xì)胞分化、毛囊發(fā)育及腫瘤發(fā)生相關(guān)。本抗體通過(guò)ELISA實(shí)驗(yàn)驗(yàn)證可特異性識(shí)別天然及重組LGR6蛋白，免疫組化（IHC）實(shí)驗(yàn)顯示其在石蠟包埋組織中能清晰定位細(xì)胞膜表達(dá)，免疫熒光（IF）染色呈現(xiàn)高分辨率的亞細(xì)胞定位信號(hào)，流式細(xì)胞術(shù)（FC）進(jìn)一步證實(shí)其適用于活細(xì)胞表面抗原檢測(cè)。推薦工作濃度為IHC/IF/FC 1:50-1:200，可滿足多樣本類型的實(shí)驗(yàn)需求。該產(chǎn)品適用于干細(xì)胞生物學(xué)、組織再生機(jī)制研究、腫瘤微環(huán)境分析及發(fā)育生物學(xué)等領(lǐng)域，尤其為探索LGR6在表皮穩(wěn)態(tài)維持、癌癥干細(xì)胞標(biāo)志物篩選等方向提供可靠檢測(cè)工具。本產(chǎn)品僅供科學(xué)研究使用，需根據(jù)實(shí)驗(yàn)體系優(yōu)化具體條件。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Leucine-rich repeat-containing G-protein coupled receptor 6, LGR6
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human LGR6
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    15G3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. May act as a tumor suppressor.
  • 基因功能參考文獻(xiàn)：
    1. Inhibition of LGR6 promoted the osteogenic differentiation of bone marrow stromal cells in vitro. PMID: 29625528
    2. Lgr6 has a tumorpromoting role in the development of colorectal cancer. PMID: 29693156
    3. Lgr6 marks mammary gland progenitor cells that can initiate tumours, and cells of luminal breast tumours required for efficient tumour maintenance. PMID: 27798604
    4. Two of the three monoclonal antibodies recognized the large N-terminal extracellular domain of leucine rich repeat containing G protein-coupled receptor 6 (LGR6), and competitively blocked the binding of R-spondin 1, which is known to be the ligand for LGR6. PMID: 28013222
    5. LGR6 expression is significantly downregulated in human masticatory mucosa during wound healing PMID: 28005267
    6. Overexpression of wild-type LGR6 in HeLa cells leads to increased cell migration. PMID: 22615920

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  • 亞細(xì)胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 19719

    OMIM: 606653

    KEGG: hsa:59352

    STRING: 9606.ENSP00000356247

    UniGene: Hs.497402