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MAPK1/MAPK3 Recombinant Monoclonal Antibody

  • 中文名稱：
    MAPK1/MAPK3重組抗體
  • 貨號：
    CSB-RA984568A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: THP-1 whole cell lysate, HEPG2 whole cell lysate,MCF7 whole cell lysate,293 whole cell lysate,K562 whole cell lysate,PC-3 whole cell lysate, Mouse brain tissue lysate
      All lanes: ERK1/2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 42,44 kDa
      Observed band size: 42,44 kDa
    • IHC image of CSB-RA984568A0HU diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.64% DAB.
    • Overlay Peak curve showing Hela cells surface stained with CSB-RA984568A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    MAPK1/MAPK3重組單克隆抗體（CSB-RA984568A0HU）靶向絲裂原活化蛋白激酶ERK1/ERK2，該家族蛋白在細胞增殖、分化及應(yīng)激響應(yīng)等關(guān)鍵信號通路中發(fā)揮核心調(diào)控作用。本抗體經(jīng)多種實驗驗證，在Western Blot中可特異性識別內(nèi)源性靶蛋白，免疫組化實驗顯示其在多種組織樣本中具有清晰的亞細胞定位，流式細胞術(shù)進一步證實其適用于活細胞表面檢測，推薦稀釋度分別為WB 1:500-1:2000、IHC 1:50-1:200、FC 1:50-1:200，同時支持ELISA平臺的高靈敏度檢測。該產(chǎn)品通過嚴格的抗原表位篩選和重組表達技術(shù)制備，具備高批次一致性和低交叉反應(yīng)性，適用于腫瘤發(fā)生機制、神經(jīng)信號傳導(dǎo)、細胞周期調(diào)控等領(lǐng)域的基礎(chǔ)研究，尤其為MAPK/ERK通路相關(guān)藥物靶點探索及疾病模型構(gòu)建提供可靠工具。本試劑僅限科研用途，需配合標準實驗條件使用。
  • Uniprot No.：
  • 基因名：
    MAPK1/MAPK3
  • 別名：
    Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1), MAPK3, ERK1 PRKM3; Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2), MAPK1, ERK2 PRKM1 PRKM2
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from Human MAPK1/MAPK3
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    29B10
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價