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MFN1 Recombinant Monoclonal Antibody

  • 中文名稱：
    MFN1重組抗體
  • 貨號：
    CSB-RA574476A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA574476A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA574476A0HU MFN1重組單克隆抗體是針對線粒體融合蛋白1（MFN1）開發(fā)的高特異性科研工具。該抗體靶向的MFN1是調(diào)控線粒體動態(tài)平衡的核心因子，通過介導(dǎo)線粒體外膜融合參與細(xì)胞能量代謝、鈣信號傳導(dǎo)及程序性死亡等重要生物學(xué)過程。經(jīng)嚴(yán)格驗(yàn)證，本品在流式細(xì)胞分析（FC）中展現(xiàn)出1:50-1:200的穩(wěn)定檢測效能，同時在ELISA平臺中具有優(yōu)異的抗原結(jié)合能力，能特異性識別天然構(gòu)象的MFN1蛋白。適用于線粒體形態(tài)學(xué)觀察、代謝性疾病模型建立、神經(jīng)退行性機(jī)制探索及心血管功能研究等基礎(chǔ)科研領(lǐng)域，尤其為活細(xì)胞水平動態(tài)監(jiān)測線粒體網(wǎng)絡(luò)重塑提供可靠檢測手段。其穩(wěn)定的批次間一致性和低交叉反應(yīng)特性，可有效支持線粒體動力學(xué)相關(guān)信號通路解析、基因敲除/過表達(dá)模型驗(yàn)證等實(shí)驗(yàn)需求，為細(xì)胞器互作研究與病理機(jī)制探索提供精準(zhǔn)工具。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Mitofusin-1 (EC 3.6.5.-) (Fzo homolog) (Transmembrane GTPase MFN1), MFN1
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human MFN1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    11G8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity.
  • 基因功能參考文獻(xiàn)：
    1. The results lead to a revised understanding of Mfn 1 as single-spanning outer membrane proteins with an Nout-Cin orientation, providing functional insight into the IMS contribution to redox-regulated fusion events. PMID: 29212658
    2. The results may suggest that TP53BP1 and MFN1 frameshift mutations and their intratumoral heterogeneity (ITH) could contribute to cancer development by inhibiting the TSG activities. PMID: 30082159
    3. The results show that a metabolic shift from glycolysis in young to mitochondrial respiration in old normal human fibroblasts occurs during chronological lifespan, and MFN1 and OPA1 regulate this process. PMID: 28758339
    4. mitochondria elongation under hypoxic condition is regulated through SIRT1-mediated MFN1 deacetylation and accumulation. PMID: 28669827
    5. Regulation of Mfn1 by MGRN1 and the proteasome modulates mitochondrial fusion. PMID: 27713096
    6. SLC25A46 is a new component in mitochondrial dynamics that serves as a regulator for MFN1/2 oligomerization. PMID: 28057766
    7. MFN1-positive expression could be seen mainly in ganglion cells after 1 week of minus lens intervention, and with time extension, more and more positive cells appeared in the rod-cone cell and bipolar cell layer, and this phenomenon could not be found in the normal control eyes. PMID: 27609161
    8. crystal structures of engineered human MFN1 containing the GTPase domain and a helical domain during different stages of GTP hydrolysis; mechanistic model for MFN1-mediated mitochondrial tethering is proposed; results shed light on the molecular basis of mitochondrial fusion and mitofusin-related human neuromuscular disorders PMID: 28114303
    9. These results suggest that MFN tethers apposing membranes, likely through nucleotide-dependent dimerization. PMID: 27920125
    10. Improper transcriptional (in)activation of mitofusin-1 and dynamin-related protein 1 during early in vitro embryo development is associated with a decrease in mitochondrial membrane potential and with embryo fragmentation. PMID: 25033890
    11. miR-19b targets 3'UTR sequences of Mfn1 genes inhibit the expression of Mfn1 PMID: 24824927
    12. In a amyotrophic lateral sclerosis transgenic mouse model, Mfn1 is significantly increased in spinal cord. PMID: 23713734
    13. A novel role for the endoplasmic reticulum-associated Gp78 ubiquitin ligase and the Mfn1 mitochondrial fusion factor in mitophagy. PMID: 23427266
    14. Knock-out of mitofusin protein Mfn1 increased the frequency of mitochondrial fission with increased lifetime of unpaired events whereas deletion of both Mfn1 and Mfn2 resulted in an instable dynamics. PMID: 22649485
    15. These results collectively suggest a role for Mfn1 in regulating the activation of Bax on the outer mitochondrial membrane in a GTPase-dependent manner. PMID: 22484496
    16. mitochondrial dynamics, particularly those mediated by the mitofusins, play a role in endothelial cell function and viability. PMID: 21839087
    17. Patterned Purkinje cell degeneration is dependent on caspase activation, leading to the marked decrease of mitofusion 1 in the transgenic Harlequin cerebellum. PMID: 20974255
    18. Our data supports a model whereby the translocation of parkin to damaged mitochondria induces the degradation of mitofusin 1 leading to impaired mitochondrial fusion PMID: 21615408
    19. The impact of mutations in endogenous PINK1 and Parkin on the ubiquitination of mitochondrial fusion and fission factors and the mitochondrial network structure, was investigated. PMID: 21408142
    20. Gbeta2 also regulated the mobility of Mfn1 on the surface of the mitochondrial membrane and affected the mitochondrial fusion. PMID: 20981029
    21. Ubiquitination of several mitochondrial proteins, including mitofusin 1 and mitofusin 2 were reduced following the silencing of parkin or PINK1. PMID: 20871098
    22. Mitofusin degradation by mitochondria-associated Parkin inhibits the fusion of damaged mitochondria with healthy mitochondria to facilitate the selective elimination of the former by autophagy. PMID: 21173115
    23. MFN1 is required for both the virus-induced redistribution of IPS-1 and IFN production. PMID: 20661427
    24. the NIC-Akt-Mfn signaling cascade identifies a pathway regulating cell-survival, independent of canonical functions associated with NIC activity PMID: 20339081
    25. Results show that Fzo homologs mitofusin 1 and 2 are ubiquitous mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. PMID: 11950885
    26. Mfn1 mediates mitochondrial fusion in human cells PMID: 12475957
    27. OPA1 functionally requires mitofusin 1 to regulate mitochondrial fusion PMID: 15509649
    28. Mfn1, Mfn2, NRF-2 and COX IV mRNA were increased 24 h post-exercise in skeletal muscle PMID: 15961417
    29. Precise interactions between a few proteins are required for mitochondrial fusion and division. Among them Drp1, Mfn1, Mfn2 and Opal are considered the most important. PMID: 17718388
    30. Fis1 and Mfn1 activities influence mitochondrial signal generation thereby insulin exocytosis. PMID: 18832378
    31. IL-6 induces Bcl-2 expression to perform cytoprotective functions in response to oxygen toxicity, and that this effect is mediated by alterations in the interactions between Bak and Mfn1/Mfn2. Bcl-2 inhibited the interaction between Bak and Mfn1. PMID: 19168699

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  • 亞細(xì)胞定位：
    Mitochondrion outer membrane; Multi-pass membrane protein.; [Isoform 2]: Cytoplasm.
  • 蛋白家族：
    TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, Mitofusin subfamily
  • 組織特異性：
    Detected in kidney and heart (at protein level). Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 18262

    OMIM: 608506

    KEGG: hsa:55669

    STRING: 9606.ENSP00000263969

    UniGene: Hs.478383