搡老女人多毛老妇女中国,日韩亚洲欧美中文高清在线,人妻少妇一区二区三区,色妞色综合久久夜夜,日本熟妇xxxx

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. MTAP Recombinant Monoclonal Antibody

MTAP Recombinant Monoclonal Antibody

  • 中文名稱：
    MTAP Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA299565A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: U-251MG whole cell lysate(20μg),HT-29 whole cell lysate(20μg), NIH/3T3 whole cell lysate(20μg)
      All lanes: MTAP antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 31 kDa
      Observed band size: 31 kDa
      Exposure time：1min20s
    • Immunofluorescence staining of Hela cell with CSB-RA299565A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA299565A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    MTAP
  • 別名：
    5' methylthioadenosine phosphorylase antibody; 5''-methylthioadenosine phosphorylase antibody; BDMF antibody; c86fus antibody; DMSFH antibody; DMSMFH antibody; Epididymis luminal protein 249 antibody; HEL 249 antibody; LGMBF antibody; MeSAdo phosphorylase antibody; Methylthioadenosine phosphorylase antibody; MSAP antibody; MTA phosphorylase antibody; MTAP antibody; MTAP_HUMAN antibody; MTAPase antibody; S methyl 5 thioadenosine phosphorylase antibody; S methyl 5' thioadenosine phosphorylase antibody; S-methyl-5''-thioadenosine phosphorylase antibody
  • 反應(yīng)種屬：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human MTAP
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    6E6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
  • 基因功能參考文獻(xiàn)：
    1. melanoma risk alleles correlate with an allele-specific hyper-methylation and down-regulation of gene expression PMID: 27761950
    2. The obtained data suggested a negative association between copy number variation of the MEAP genes and Atherothrombotic stroke. PMID: 28477716
    3. High MTAP expression is associated with prostate cancer. PMID: 26910893
    4. Results indicate complex rearrangements involving CDKN2A locus and frequent occurrence of fusion transcripts involving methylthioadenosine phosphorylase (MTAP) and ANRIL genes. PMID: 26909863
    5. The SNP rs7023954 of methylthioadenosine phosphorylase co-dominantly expressed alleles revealed no difference in the conversion rate of 5'-methylthioadenosine to adenine and 5-methylthioribose-1-phosphate, indicating that this known enzymatic activity does not modulate the tumor suppressive function of methylthioadenosine phosphorylase. PMID: 27479139
    6. Data demonstrate that MTAP deficiency leads to accumulation of MTA inducing deregulation of central cellular pathways that control cell fate and contributes to the onset of a proliferative and antiapoptotic phenotype in the liver. PMID: 26819315
    7. methionine adenosyltransferase II alpha (MAT2A), and the arginine methyltransferase, PRMT5, as vulnerable enzymes in cells with MTAP deletion. PMID: 27068473
    8. MTAP expression was significantly higher in Luminal-A breast tumors than in triple negative breast neoplasms, suggesting the lack of expression in more aggressive breast tumors. PMID: 26751376
    9. study to describe MTAP expression in a series of Pilocytic astrocytomas and relate it to the clinicopathological features of the patients; found MTAP expression is retained in Pilocytic astrocytomas and is not an outcome predictor for these tumors PMID: 26088413
    10. MTAP deficiency was predictive of worse disease-specific survival and distant metastasis-free survival, suggesting its role in disease progression and as an independent prognostic biomarker of nasopharyngeal carcinoma PMID: 26656376
    11. In the MTAP-deficient cases, the homozygous deletion of MTAP predicted adverse outcome. In MTAP-deficient cells, MTAP reexpression inhibited cell migration and invasion, proliferation and anchorage-independent colony formation and downregulated cyclin D1. PMID: 25426549
    12. MTAP expression is an independent prognostic factor and has greater prognostic significance than p16 expression in non-small cell lung cancer and concordant loss of MTAP and p16 expression indicates poor outcomes in lung cancer patients. PMID: 24969958
    13. MTAP-mediated regulation of methylthioadenosine links polyamine metabolism with NF-kappaB activation and apoptosis in hepatic stellate cells. PMID: 24324622
    14. SNP rs10118757 was associated with CAD risk in a Chinese Han population, indicating that MTAP gene may play a potential role in the pathophysiological process of CAD. PMID: 23462334
    15. Homozygous deletion of MTAP gene is associated with haploid lymphoblastic leukemia. PMID: 23508829
    16. Authors report here that a high percentage of t-cell lymphoma lack the enzyme methylthioadenosine phosphorylase (MTAP). PMID: 23040436
    17. MTAP gene might be involved in the etiology of myocardial infarction in Chinese Han ethnicity. PMID: 20302706
    18. Letter: inactivation of MTAP expression seems to be an important step in the development and progression of malignant melanoma. of PMID: 23000879
    19. Data indicate that a signature of seven biomarkers (Bax, Bcl-X, PTEN, COX-2, beta-Catenin, MTAP and CD20) was found to be an independent negative predictor for overall and recurrence-free survival in cutaneous malignant melanoma (MM). PMID: 22685558
    20. A large negative heat capacity change of -600 cal/(mol K) upon inhibitor binding to MTAP is consistent with altered hydrophobic interactions and release of water. PMID: 21985704
    21. Polymorphisms in nevus-associated genes MTAP is associated with invasive cutaneous melanoma. PMID: 21962134
    22. The present study suggests that MTAP plays an important role in the regulation of gastric carcinogenesis. PMID: 21412930
    23. Expression of MTAP is significantly higher in human colorectal cancer than in normal colorectal tissues. Demethylation of MTAP promoter may play an important role in up-regulating MTAP expression. PMID: 19622299
    24. The expression of MTAP protein in non-small cell lung cancer tissue was significantly lower than that in paracarcinomous tissue and borderline lung tissue. PMID: 21342647
    25. MTAP deficiency results in accumulation of 5'-deoxy-5'-methylthioadenosine, which is associated with increased tumorigenicity. PMID: 21356366
    26. CDKN2B (p15(INK4b)), CDKN2A (p16(INK4a), p14(ARF)), and MTAP are abundantly expressed in atherosclerotic lesions. PMID: 20637465
    27. MTDIA antitumor activity in xenografts supports MTAP as a target for lung cancer therapy. PMID: 21135097
    28. Single nucleotide polymorphism in MTAP gene is associated with nevi. PMID: 20574843
    29. Detection of CDKN2A-MTAP co-deletion in peritoneal mesotheliomas can help identify those patients who may have an unfavorable outcome PMID: 20081810
    30. MTAP homozygous deletion, the predominant mechanism to deplete protein expression, is present in 17% of gastrointestinal stromal tumors PMID: 19887491
    31. Methylthioadenosine phosphorylase, a gene frequently codeleted with p16(cdkN2a/ARF), acts as a tumor suppressor in a breast cancer cell line. PMID: 12438261
    32. Results suggest an important role of methylthioadenosine phosphorylase inactivation in the development of melanomas. PMID: 12875987
    33. Methylthioadenosine phosphorylase regulates ornithine decarboxylase by production of downstream metabolites PMID: 14506228
    34. MTAP is not expressed in normal human colonic epithelium but is strongly upregulated in colon carcinoma. PMID: 15492751
    35. epigenetic mechanisms, involving DNA methylation and histone deacetylation, may play a role in the silencing of MTAP gene expression in hepatocarcinoma PMID: 15511635
    36. MTAP activity is frequently lost, and ODC activity is frequently elevated in both pancreatic adenocarcinoma and neuroendocrine tumors PMID: 15534104
    37. MTAP expression is lost in approximately 30% of infiltrating pancreatic cancers and in a lower percentage of other periampullary neoplasms; loss is a result of homozygous deletions encompassing both the MTAP and p16INK4A/CDKN2A genes PMID: 15662124
    38. Results demonstrated concordant loss of MTAP and p16 protein expression in pancreatic intraepithelial neoplasia. PMID: 15832197
    39. MTAP inactivation is associated with hepatocellular carcinoma development and invasiveness PMID: 16081515
    40. Selective loss at the 3' end of MTAP was observed in head and neck squamous cell carcinoma cell line UMSCC-11A. PMID: 16618910
    41. The frequency of Methylthioadenosine phosphorylase (MTAP) deletions in conventional, grade II chondrosarcomas by fluorescence in situ hybridization (FISH) analysis was investigated. PMID: 16631464
    42. Methylthioadenosine phosphorylase (MTAP) gene deletion and lack of protein expression are associated with poor prognosis in mantle cell lymphoma. PMID: 16778103
    43. Regulation of MTAP by reactive oxygen species might participate in the redox regulation of the methionine catabolic pathway in the liver PMID: 18237276
    44. analysis of methylthioadenosine phosphorylase (MTAP) deficiency in non-small cell lung carcinoma PMID: 18555557
    45. This study demonstrates for the first time that polymorphisms in CDKN2B and MTAP gene may influence the risk of myocardial infarction in Chinese. PMID: 19272367
    46. The G allele of rs10118757 was associated with an increased risk of stroke in Han Chinese even after controlling for confounding factors. The GA+GG genotype was associated with the increased risk of an undetermined subtype of ischemic stroke. PMID: 19427650

    顯示更多

    收起更多

  • 相關(guān)疾?。?/div>
    Diaphyseal medullary stenosis with malignant fibrous histiocytoma (DMSMFH)
  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    PNP/MTAP phosphorylase family, MTAP subfamily
  • 組織特異性：
    Ubiquitously expressed.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 7413

    OMIM: 112250

    KEGG: hsa:4507

    STRING: 9606.ENSP00000369519

    UniGene: Hs.193268