NDUFB8 Recombinant Monoclonal Antibody
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中文名稱:NDUFB8 Recombinant Monoclonal Antibody
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貨號:CSB-RA985048A0HU
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規(guī)格:¥1320
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圖片:
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Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg), LO2 whole cell lysate(30μg), A431 whole cell lysate(30μg), PC-3 whole cell lysate(30μg), Hela whole cell lysate(30μg), JURKAT whole cell lysate(30μg), A549 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Mouse heart tissue lysate(30μg)
All lanes: NDUFB8 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa
Exposure time:20s -
IHC image of CSB-RA985048A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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IHC image of CSB-RA985048A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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其他:
產(chǎn)品詳情
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Uniprot No.:
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基因名:
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別名:ASHI antibody; CI-ASHI antibody; Complex I ASHI subunit antibody; Complex I-ASHI antibody; mitochondrial antibody; NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody; NADH-ubiquinone oxidoreductase ASHI subunit antibody; NDUB8_HUMAN antibody; NDUFB8 antibody
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反應(yīng)種屬:Human, Mouse
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免疫原:A synthesized peptide derived from human NDUFB8
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免疫原種屬:Homo sapiens (Human)
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標記方式:Non-conjugated
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克隆類型:Monoclonal
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抗體亞型:Rabbit IgG
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純化方式:Affinity-chromatography
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克隆號:8B11
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 -
Protocols:
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儲存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
相關(guān)產(chǎn)品
靶點詳情
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功能:Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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基因功能參考文獻:
- NDUFB8 is a relevant gene in childhood-onset mitochondrial disease. PMID: 29429571
- Results describe PTEN and NDUFB8 aberrations in cervical cancer tissue. PMID: 17727244
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亞細胞定位:Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.
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蛋白家族:Complex I NDUFB8 subunit family
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數(shù)據(jù)庫鏈接:
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