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NUDT5 Recombinant Monoclonal Antibody

  • 中文名稱：
    NUDT5重組抗體
  • 貨號：
    CSB-RA936419A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate
      All lanes: NUDT5 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 25 kDa
      Observed band size: 36 kDa
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA936419A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA936419A0HU重組單克隆抗體是針對NUDT5靶點研發(fā)的高特異性科研工具，該抗體采用先進(jìn)的單克隆重組技術(shù)制備，具有優(yōu)異的批間一致性。NUDT5作為Nudix水解酶家族成員，在核苷酸代謝調(diào)控、氧化應(yīng)激應(yīng)答及DNA損傷修復(fù)等生物學(xué)過程中發(fā)揮重要作用。經(jīng)嚴(yán)格驗證，該抗體在Western Blot實驗中可清晰識別內(nèi)源性NUDT5蛋白，F(xiàn)C檢測中能有效區(qū)分陽性與陰性細(xì)胞群體，并在ELISA體系中展現(xiàn)高親和力結(jié)合特性。推薦使用稀釋度為WB 1:500-1:2000、FC 1:50-1:200，適用于細(xì)胞裂解液、組織樣本及活細(xì)胞表面標(biāo)記等多種實驗體系。本產(chǎn)品主要服務(wù)于分子代謝研究、腫瘤相關(guān)機(jī)制探索以及氧化應(yīng)激調(diào)控等基礎(chǔ)科研領(lǐng)域，為研究NUDT5在能量穩(wěn)態(tài)維持、基因組穩(wěn)定性調(diào)控等過程中的功能提供可靠檢測工具。
  • Uniprot No.：
  • 基因名：
    NUDT5
  • 別名：
    ADP-sugar pyrophosphatase (EC 3.6.1.13) (8-oxo-dGDP phosphatase) (EC 3.6.1.58) (Nuclear ATP-synthesis protein NUDIX5) (EC 2.7.7.96) (Nucleoside diphosphate-linked moiety X motif 5) (Nudix motif 5) (hNUDT5) (YSA1H), NUDT5, NUDIX5
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human NUDT5
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    10D2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Enzyme that can either act as an ADP-sugar pyrophosphatase in absence of diphosphate or catalyze the synthesis of ATP in presence of diphosphate. In absence of diphosphate, hydrolyzes with similar activities various modified nucleoside diphosphates such as ADP-ribose, ADP-mannose, ADP-glucose, 8-oxo-GDP and 8-oxo-dGDP. Can also hydrolyze other nucleotide sugars with low activity. In presence of diphosphate, mediates the synthesis of ATP in the nucleus by catalyzing the conversion of ADP-ribose to ATP and ribose 5-phosphate. Nuclear ATP synthesis takes place when dephosphorylated at Thr-45. Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming. Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that targeting nudix hydrolase 5 (NUDT5) may represent a promising new therapeutic approach for breast cancer treatment. PMID: 29343827
    2. In the presence of pyrophosphate, ADP-ribose is used by the pyrophosphatase NUDIX5 to generate nuclear ATP. PMID: 27257257
    3. The NUDT5 protein could play significant roles in the prevention of RNA oxidation and survival in human fibroblast cells. PMID: 23581889
    4. Results suggest that the NUDT5 protein may play significant roles in regulating the G1-S transition in HeLa cells. PMID: 22200976
    5. The human NUDT5, which has an intrinsic activity to cleave ADP sugars to AMP and sugar phosphate, possesses the ability to degrade 8-oxo-dGDP to the monophosphate. PMID: 21389046
    6. The broad substrate specificity of hNUDT5 is achieved by a diversity of not only substrate recognition, but also hydrolysis mechanisms. PMID: 21768126
    7. human MTH1, MTH2, and NUDT5 proteins act as a defense against the mutagenesis induced by oxidized dGTP. PMID: 20144704
    8. NUDT5 protein eliminates various oxidized deoxyribonucleoside diphosphates from the nucleotide pool and prevents their toxic effects. PMID: 19699693
    9. degrades 8-oxo-dGDP to 8-oxo-dGMP, an unusable form for DNA synthesis, and promotes the cleavage of 8-oxo-dGTP by MTH1 to yield 8-oxo-dGMP also PMID: 12717453
    10. Results report the crystal structure of hNUDT5 in complex with a non-hydrolyzable ADPR analogue, alpha,beta-methyleneadenosine diphosphoribose, and three Mg(2+) ions representing the transition state of the enzyme during catalysis. PMID: 18462755

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  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    Nudix hydrolase family
  • 組織特異性：
    Widely expressed. Most abundant in liver.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 8052

    OMIM: 609230

    KEGG: hsa:11164

    STRING: 9606.ENSP00000419628

    UniGene: Hs.555956