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PDE2A Recombinant Monoclonal Antibody

  • 中文名稱：
    PDE2A重組抗體
  • 貨號(hào)：
    CSB-RA150831A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Immunofluorescence staining of HepG2 cell with CSB-RA150831A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 569-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA150831A0HU PDE2A重組單克隆抗體是針對(duì)磷酸二酯酶2A（PDE2A）靶點(diǎn)開發(fā)的高特異性科研工具，適用于酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）和免疫熒光（IF）檢測(cè)。PDE2A作為cAMP/cGMP信號(hào)通路的關(guān)鍵調(diào)控酶，參與細(xì)胞第二信使代謝，在神經(jīng)可塑性、心血管功能及激素分泌等生理過(guò)程中發(fā)揮重要作用。本抗體基于重組表達(dá)技術(shù)制備，具有優(yōu)異的批次一致性和低交叉反應(yīng)性，經(jīng)嚴(yán)格驗(yàn)證可在免疫熒光實(shí)驗(yàn)中清晰呈現(xiàn)細(xì)胞內(nèi)源性PDE2A蛋白的亞定位特征，推薦使用稀釋比例為1:50-1:200，實(shí)驗(yàn)時(shí)需根據(jù)樣本類型優(yōu)化通透處理?xiàng)l件。該產(chǎn)品能夠有效支持PDE2A在神經(jīng)系統(tǒng)、內(nèi)分泌系統(tǒng)或心血管相關(guān)細(xì)胞模型中的蛋白表達(dá)分析，適用于研究環(huán)核苷酸信號(hào)調(diào)控機(jī)制、藥物靶點(diǎn)篩選及疾病相關(guān)分子通路探索等基礎(chǔ)科研領(lǐng)域，為體外實(shí)驗(yàn)提供穩(wěn)定可靠的檢測(cè)方案。
  • Uniprot No.：
  • 基因名：
  • 別名：
    cGMP-dependent 3',5'-cyclic phosphodiesterase (EC 3.1.4.17) (Cyclic GMP-stimulated phosphodiesterase) (CGS-PDE) (cGSPDE), PDE2A
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human PDE2A
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    11C6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IF 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line).; Regulates mitochondrial cAMP levels and respiration. Involved in the regulation of mitochondria morphology/dynamics and apoptotic cell death via local modulation of cAMP/PKA signaling in the mitochondrion, including the monitoring of local cAMP levels at the outer mitochondrial membrane and of PKA-dependent phosphorylation of DNM1L.
  • 基因功能參考文獻(xiàn)：
    1. the well-known interaction between AIP and 2 different isoforms of phosphodiesterases (PDEs), PDE2A3 and PDE4A5, is of particular interest. While the interaction with over-expressed AIP does not seem to affect PDE2A3 function, the reported effect on PDE4A5 is, in contrast, reduced enzymatic activity. PMID: 28427099
    2. Lu AF33241 represents a novel PDE2A/PDE10A inhibitor tool which can penetrate the blood brain barrier. PMID: 25941078
    3. Epigenetic regulation of phosphodiesterases 2A and 3A underlies compromised beta-adrenergic Signaling in an induced pluripotent stem cell model of dilated cardiomyopathy. PMID: 26095046
    4. curcumin exerts its in vitro anti-angiogenic and in vivo anti-tumour properties through combined PDE2 and PDE4 inhibition PMID: 25230992
    5. PDE2A is involved in growth and invasion of human malignant melanoma PMP cells. PMID: 24705027
    6. This study reports the X-ray crystal structure of PDE2A in complex with a highly selective, nanomolar inhibitor (BAY60-7550) at 1.9 A resolution, and the structure of apo PDE2 at 2.0 A resolution. PMID: 23899287
    7. PDE2 overexpression in healthy cardiomyocytes reduces the rise in cyclic AMP levels and L-type calcium current amplitude, and abolishes the inotropic effect following acute beta-adrenergic receptor stimulation. PMID: 23810893
    8. Our results demonstrated that the PDE2A2 variant carrying point mutations is expressed in PMP cells and may affect cell cycle progression by modulating cyclin A expression. PMID: 23381931
    9. found in smooth muscle wall of blood vessels transversing the clitoral supepithelial and stromal space PMID: 21697861
    10. Active site similarity between human and Plasmodium falciparum phosphodiesterases: considerations for antimalarial drug design PMID: 21766240
    11. the phosphodiesterase 2A isoform localized to mitochondria regulates respiration PMID: 21724846
    12. Inhibition of PDE2 resulted in higher levels of intracellular cAMP than inhibition of PDE3A suggesting this PDE may be the more important regulator of cAMP in human platelets. PMID: 12038792
    13. TNF-alpha-mediated up-regulation of PDE2 may destabilize endothelial barrier function in sepsis PMID: 15650061
    14. Phosphodiesterase 2 mediates redox-sensitive endothelial cell proliferation and angiogenesis by thrombin via Rac1 and NADPH oxidase 2. PMID: 19390057
    15. report the X-ray crystal structures of a PDE protein that includes both catalytic and regulatory domains, namely, PDE2A containing the N-terminal GAF domains and the catalytic domain. PMID: 19828435

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  • 亞細(xì)胞定位：
    [Isoform PDE2A3]: Cell membrane; Lipid-anchor.; [Isoform PDE2A2]: Mitochondrion matrix. Mitochondrion inner membrane. Mitochondrion outer membrane.; [Isoform PDE2A1]: Cytoplasm.; [Isoform 5]: Mitochondrion.
  • 蛋白家族：
    Cyclic nucleotide phosphodiesterase family, PDE2 subfamily
  • 組織特異性：
    Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 8777

    OMIM: 602658

    KEGG: hsa:5138

    STRING: 9606.ENSP00000334910

    UniGene: Hs.503163