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  4. PKN2 Recombinant Monoclonal Antibody

PKN2 Recombinant Monoclonal Antibody

  • 中文名稱：
    PKN2重組抗體
  • 貨號：
    CSB-RA921617A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate
      All lanes: PKN2 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 113, 117, 112 kDa
      Observed band size: 130 kDa
    • IHC image of CSB-RA921617A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of MCF-7 cell with CSB-RA921617A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 509-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing MCF7 cells stained with CSB-RA921617A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    PKN2重組單克隆抗體（CSB-RA921617A0HU）是針對蛋白激酶N2（Protein Kinase N2）開發(fā)的高特異性試劑，適用于多種實驗場景。PKN2作為AGC激酶家族成員，在Rho信號通路中發(fā)揮關(guān)鍵作用，參與調(diào)控細(xì)胞骨架重組、細(xì)胞極性建立及凋亡過程，其異常表達與腫瘤侵襲、神經(jīng)發(fā)育等領(lǐng)域相關(guān)研究密切相關(guān)。本抗體經(jīng)ELISA、Western Blot（WB）、免疫組化（IHC）、免疫熒光（IF）及流式細(xì)胞術(shù)（FC）嚴(yán)格驗證，在WB實驗中可特異性識別天然及重組PKN2蛋白，推薦稀釋比例1:500-1:2000；IHC實驗顯示其對石蠟包埋組織切片中目標(biāo)蛋白的精準(zhǔn)定位能力（1:50-1:200）；IF和FC實驗分別在細(xì)胞固定樣本與活細(xì)胞表面檢測中呈現(xiàn)高信噪比（均適用1:50-1:200稀釋度）。該產(chǎn)品適用于探索PKN2在細(xì)胞遷移機制、腫瘤微環(huán)境調(diào)控、神經(jīng)元突觸可塑性等領(lǐng)域的分子功能，為體外研究提供可靠工具。所有實驗數(shù)據(jù)經(jīng)多物種樣本驗證，批次間穩(wěn)定性優(yōu)異，適用于基礎(chǔ)科研中蛋白互作分析、信號通路解析及疾病模型構(gòu)建等需求。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Serine/threonine-protein kinase N2 (EC 2.7.11.13) (PKN gamma) (Protein kinase C-like 2) (Protein-kinase C-related kinase 2), PKN2, PRK2 PRKCL2
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human PKN2
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    20A8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis.; (Microbial infection) Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.
  • 基因功能參考文獻：
    1. The expression of PKN2 in colon cancer cells suppresses tumor associated M2 macrophage polarization and tumor growth. PMID: 29368606
    2. PKN2 knockdown in vitro decreased insulin-stimulated glucose uptake, incorporation into glycogen, and oxidation. Thus, PKN2 alters key signaling pathways and transcriptional networks to regulate glucose and lipid metabolism. PMID: 28720584
    3. Helicobacter pylori CagA interacts with PRK2 and inhibits its kinase activity. PMID: 26041307
    4. findings demonstrate that Yersinia enterocolitica rYopM interacts with RSK1 and PRK2 following cell-penetration PMID: 25513777
    5. Regulation of protein kinase C-related protein kinase 2 (PRK2) by an intermolecular PRK2-PRK2 interaction mediated by Its N-terminal domain. PMID: 22511787
    6. these findings suggest that Hsp90 plays a critical role in the regulation of HCV RNA polymerase phosphorylation via the PDK1-PRK2 signaling pathway. PMID: 22490666
    7. Rho binding is essential for PRK2 function and facilitates PRK2 recruitment to junctions. Kinase-dead PRK2 acts as a dominant-negative mutant and prevents apical junction formation. PMID: 20974804
    8. PRK2/PKN2, is an essential regulator of both entry into mitosis and exit from cytokinesis in HeLa S3 cells, required for abscission of the midbody at the end of the cell division cycle and for phosphorylation and activation of Cdc25B. PMID: 17332740
    9. These observations highlight elements of Nef's functional complexity and demonstrate previously unsuspected structural requirements for PAK-2 activation and MHC-1 down-modulation in Nef's flexible N- and C-terminal regions. PMID: 17632197
    10. Little evidence of association was observed between SNPs in PKN2 and type 2 diabetes in African Americans. PMID: 18443202
    11. the last seven amino acid residues at the C-terminus of PRK2 are not required for the activation of the kinase by RhoA in vitro, however, the extreme C-terminal segment is critical for the full activation of PRK2 by RhoA in cells PMID: 18835241

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Membrane. Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Cleavage furrow. Midbody. Cell junction.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
  • 組織特異性：
    Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9406

    OMIM: 602549

    KEGG: hsa:5586

    STRING: 9606.ENSP00000359552

    UniGene: Hs.440833