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RAB25 Recombinant Monoclonal Antibody

  • 中文名稱：
    RAB25重組抗體
  • 貨號：
    CSB-RA284745A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Immunofluorescence staining of PC-3 cell with CSB-RA284745A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 516-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA284745A0HU RAB25重組單克隆抗體是針對RAS癌基因家族成員RAB25研發(fā)的高特異性檢測工具。該抗體可特異性識別人類源RAB25蛋白，經(jīng)嚴格驗證適用于ELISA和免疫熒光（IF）實驗，其中IF實驗推薦使用1:50-1:200的稀釋比例，能清晰顯示目標蛋白在細胞內(nèi)的亞細胞定位特征。RAB25作為小GTP酶家族重要成員，通過調(diào)控囊泡運輸和細胞膜蛋白分選參與腫瘤發(fā)生發(fā)展，其異常表達與卵巢癌、乳腺癌等上皮源性腫瘤的轉(zhuǎn)移及耐藥性密切相關。本產(chǎn)品采用重組表達抗原免疫制備，經(jīng)多批次質(zhì)控驗證顯示優(yōu)異的批間一致性，在IF實驗中能準確呈現(xiàn)RAB25在細胞質(zhì)或膜相關結(jié)構(gòu)的分布特征，為研究腫瘤細胞極性建立、受體循環(huán)調(diào)控及上皮-間質(zhì)轉(zhuǎn)化等關鍵生物學過程提供可靠工具。適用于癌癥機制研究、細胞內(nèi)膜運輸通路分析以及腫瘤生物標志物篩選等科研領域，特別在三維細胞培養(yǎng)模型或類器官研究中具有重要應用價值。實驗建議配合陽性對照樣本以確認檢測體系有效性，具體實驗條件可參照產(chǎn)品說明書進行優(yōu)化。
  • Uniprot No.：
  • 基因名：
    RAB25
  • 別名：
    Ras-related protein Rab-25 (CATX-8), RAB25, CATX8
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human RAB25
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    27H3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IF 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis.
  • 基因功能參考文獻：
    1. ZEB2 directly binds to E-box sequences in the RAB25 promoter.ZEB2 stably represses RAB25 expression through epigenetic regulation by SIRT1 during epithelial-to-mesenchymal transition. PMID: 30445998
    2. Results indicated that miR-577 suppressed EMT by inhibiting Rab25 expression in breast cancer (BC). MiR-577 and Rab25 are considered potential targets of BC treatment. PMID: 29524309
    3. Rab25 is amplified and enhances aggressiveness in luminal B cancers while in claudin-low tumors, Rab25 is lost indicating possible anti-tumor functions PMID: 27259233
    4. Our results suggest that Rab25 tumourigenic potential and chemoresistance relies on HIF1 activity in aggressive and metastatic ovarian cancer PMID: 26967059
    5. The results from this analysis indicated that Rab11a, Rab11c(Rab25) and Rab14 were expressed in a wide range of cell lines, including the human placental trophoblastic BeWo cell line. PMID: 28922401
    6. Data show that Rab25 is responsible for RIN1-induced EGFR signaling and tumor malignancy in clear cell renal cell carcinoma cell. PMID: 28612496
    7. Promoter methylation results in downregulation of RAB25 expression in oropharyngeal squamous cell carcinoma. Decreased Rsb25 expression is associated with lymph node metastasis. PMID: 27379752
    8. Rab25 may act as a tumor promoter. PMID: 28281975
    9. Rab25 was upregulated in hepatocellular carcinoma tissues and cells compared with normal liver tissues and cell lines. Rab25 overexpression correlated with advanced tumor stage and nodal metastasis.Rab25 depletion blocked cell growth rate, inhibited colony formation, downregulated AKT phosphorylation, inhibited the Wnt signaling pathway and its target genes, and negatively regulated the invading ability of HCC cells. PMID: 26692100
    10. Rab25 expression is a potential prognostic index for advanced NSCLC patients and its inhibition may improve chemosensitization in NSCLC treatment. PMID: 26535714
    11. These results showed that high levels of Rab25 expression were significantly correlated with renal cell carcinoma invasion classification (P < 0.01), lymph-node metastasis (P < 0.001), and pathological stage (P < 0.01). PMID: 25686498
    12. High RAB25 expression is associated with good clinical outcome in patients with locally advanced head and neck squamous cell carcinoma. PMID: 24403269
    13. the crystal structure of Rab25 in complex with the C-terminal region of FIP2, which consists of a central dimeric FIP2 coiled-coil that mediates a heterotetrameric Rab25-(FIP2)2-Rab25 complex. PMID: 24056041
    14. Overexpression of Rab25 contributes to metastasis of bladder cancer through induction of epithelial-mesenchymal transition and activation of Akt/GSK-3beta/Snail signaling. PMID: 23722651
    15. These findings suggest that Rab25 plays an important role in tumor migration and metastasis, and that understanding its function may lead to the development of new strategies to prevent metastasis in oral cancer patients. PMID: 23340300
    16. Rab25 controls intestinal cell polarity through the regulation of gene expression PMID: 23345591
    17. RAB25 has been implicated in various cancers, with reports presenting it as both an oncogene and a tumour-suppressor gene. [Review] PMID: 23176489
    18. Studies using lentiviral-based overexpression and suppression systems lent support of Rab25 to function as an important tumor suppressor with both anti-invasive and -angiogenic abilities, through a deregulated FAK-Raf-MEK1/2-ERK signaling pathway. PMID: 22991305
    19. data showed Rab25 was highly expressed in all subtypes of epithelial ovarian cancers, and two subtypes of germ cell tumors, but not in sex cord stromal tumors; Rab25 expression was not correlated with peritoneal metastasis of ovarian cancer PMID: 23030522
    20. higher level of Rab25 was associated with lymphatic metastasis, specifically in ER and PR-positive breast cancer. PMID: 22644676
    21. Rab25 acts predominantly in the small-scale fast recycling within the tips of the cell. Our results further support the idea of Rab25 as a promoter of tumor development PMID: 22613965
    22. Rab25 induced the accumulation of glycogen in epithelial cancer cells, a process not previously identified. (RAB25) PMID: 22253197
    23. Rab25 effectively differentiates Mullerian carcinomas from malignant mesothelioma PMID: 22249560
    24. The present study delineates the fundamental elements of a core promoter structure that will be helpful for future studies regarding the regulation of RAB25 gene. PMID: 21075212
    25. Loss of Rab25 is associated with triple-negative breast cancer. PMID: 19795443
    26. Rab25 may function as a tumor suppressor in intestinal epithelial cells through regulation of protein trafficking to the cell surface. PMID: 20197623
    27. Rab25 signalling pathway plays a role in the regulation of cell proliferation and apoptosis in ovarian cancer cells, which indicates that the Rab25 gene plays a definite role in the development and aggressiveness of human ovarian cancer. PMID: 17393986
    28. Rab25 contributes to tumor progression by directing the localization of integrin-recycling vesicles and thereby enhancing the ability of tumor cells to invade the extracellular matrix. PMID: 17925226

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  • 亞細胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Cell projection, pseudopodium membrane. Cytoplasmic vesicle.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 組織特異性：
    Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancer
  • 數(shù)據(jù)庫鏈接：

    HGNC: 18238

    OMIM: 612942

    KEGG: hsa:57111

    STRING: 9606.ENSP00000354376

    UniGene: Hs.632469