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  4. RAB27A Recombinant Monoclonal Antibody

RAB27A Recombinant Monoclonal Antibody

  • 中文名稱：
    RAB27A重組抗體
  • 貨號：
    CSB-RA285197A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Overlay Peak curve showing PC3 cells stained with CSB-RA285197A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • 產品描述：
    CSB-RA285197A0HU RAB27A重組單克隆抗體是針對RAB27A蛋白開發(fā)的高特異性檢測工具，該靶點作為RAB小G蛋白家族成員，在囊泡運輸調控、胞吐作用及細胞分泌過程中發(fā)揮關鍵作用，其功能異常與黑色素瘤發(fā)生發(fā)展、免疫細胞活性調控及神經遞質釋放等生物學過程密切相關。本產品經嚴格驗證適用于ELISA和流式細胞術（FC）檢測，其中FC實驗推薦使用1:50-1:200稀釋比例，實驗數(shù)據(jù)顯示其能有效識別天然構象及重組表達的RAB27A蛋白，且具備優(yōu)異的批間一致性。該抗體可廣泛應用于腫瘤細胞分泌機制研究、免疫細胞胞吐作用分析以及神經內分泌調控等基礎科研領域，特別適用于黑色素瘤模型中的囊泡運輸研究、免疫細胞（如T細胞/NK細胞）的細胞毒性功能評估，以及神經細胞或內分泌細胞分泌通路的分子機制探索，為相關領域的蛋白功能研究提供可靠檢測工具。
  • Uniprot No.：
  • 基因名：
    RAB27A
  • 別名：
    Ras-related protein Rab-27A (Rab-27) (GTP-binding protein Ram), RAB27A, RAB27
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human RAB27A
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    2E8
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
  • 基因功能參考文獻：
    1. These results collectively suggest that the active form of Rab27a enhances human parainfluenza virus type 2 growth by promoting transport of F and HN proteins to the plasma membrane. PMID: 29374787
    2. A novel Rab27a mutation binds melanophilin, but not Munc13-4, causing immunodeficiency without albinism. PMID: 27016801
    3. We reported the first patient with GS2 in Thailand. The patient was compound heterozygous for two novel mutations, c.109A>T (p.K37X) and c.318T>G (p.S106R). We reported the first case of a successful prenatal diagnosis for GS2. The result revealed that the fetus carried the p.K37X, but not the p.S106R (Fig. C), suggesting that the fetus would be unaffected with GS2. PMID: 28484936
    4. Findings indicate that rab GTP-binding proteins Rab27A and Rab27B play significant roles in cell invasion, proliferation, and apoptosis, as well as in chemotherapy resistance. PMID: 28902788
    5. Here, we report on a new RAB27A genetic anomaly observed in seven Saudi Arabia families that had remained negative after extensive molecular genomic DNA testing. Linkage analysis and targeted sequencing of the RAB27A genomic region in several of these patients led to the identification of a common homozygous tandem duplication of 38 kb affecting exon 2-5 and resulting in a premature stop codon PMID: 28585352
    6. In comparison with the normal tissues, miR-182-5p is expressed at a higher level in gastric cancer (GC) tissues, while RAB27A is expressed at a lower level in cancerous tissues. The down-regulation of miR-182-5p and up-regulation of RAB27A can significantly decrease the viability, migration, invasion, and mitosis of HGC-27 cells. PMID: 28546229
    7. Rab27A is mediated by NF-kappaB and promotes stemness of colon cancer cells via up-regulation of cytokine secretion PMID: 27556511
    8. These results demonstrated that miR-145 has an inhibitory role in TNBC malignancy by targeting MMP11 and Rab27a, which might be potential therapeutic and diagnostic targets for TNBC. PMID: 27364572
    9. These signaling pathways can trigger several secretion modes including single WPB release and multigranular exocytosis. In this review we will give an overview of the WPB lifecycle from biogenesis to secretion and we will discuss several deficiencies that affect the WPB lifecycle. [review] PMID: 28004844
    10. RAB27A, RAB27B and VPS36 are frequently underexpressed in advanced prostate cancer and are inversely correlated with prostate cancer outcome. There seems to be a close relationship in the expression of RAB27A, RAB27B and VPS36, with RAB27A and RAB27B being dependent on VPS36. PMID: 28197629
    11. identified small molecule inhibitors of Rab27a-JFC1 binding that were also active in cell-based neutrophil-specific exocytosis assays, demonstrating the druggability of Rab GTPases and their effectors. PMID: 27702998
    12. MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A. PMID: 28043921
    13. decreased expression of Rab27A and Rab27B, especially Rab27A, closely correlated with tumor progression and are valuable prognostic indicators in colorectal cancer patients. PMID: 26760980
    14. We report two unrelated teenagers with hemophagocytic lymphohistiocytosis and an identical heterozygous RAB27A mutation (c.259G-->C). PMID: 26880764
    15. Data suggest that microRNA miR-582-5p may function as a tumor suppressor in the development of colorectal carcinoma (CRC) by targeting RAS-related GTP-binding protein (Rab27a), indicating a novel therapeutic strategy for patients with CRC. PMID: 26384136
    16. Experimental data are reviewed with a focus on the secretory Rab27 family of small GTPases and their implications in cancer progression. PMID: 23665896
    17. miR-122 has a Rab27a-dependent function in the hepatitis C virus lifecycle PMID: 26305877
    18. Rab27A may be used as a valuable prognostic biomarker for colorectal carcinoma patients. PMID: 26070933
    19. Our data indicate that RAB27A expression is an independent prognostic marker for pancreatic ductal adenocarcinoma PMID: 25428385
    20. This study identified a common variant located in RAB27A gene influencing FeNO levels specifically in adults and with a biological relevance to the regulation of FeNO levels. PMID: 25431337
    21. These studies highlight the need for RAB27A sequencing in patients with FHL with normal pigmentation and identify a critical binding site for Munc13-4 on Rab27a, revealing the molecular basis of this interaction. PMID: 25312756
    22. Data show that cystic fibrosis (CF) neutrophils release less secondary and tertiary granule components and that activation of the low-molecular-mass GTP-binding protein Rab27a, involved in the regulation of granule trafficking, is defective. PMID: 24934256
    23. Rab27a was identified as a prognostic biomaker by mRNA profiling, correlated with malignant progression and subtype preference in gliomas. PMID: 24587032
    24. Silencing of the exocytotic RAB family members RAB27A or RAB27B halted miR23b and miR921 secretion and reduced cellular invasion. PMID: 25261234
    25. EPI64, a candidate GAP that is specific for Rab27. PMID: 24673604
    26. Rab27a plays an important role in NET formation induced by both Candida albicans infection and PMA treatment by regulating ROS production. PMID: 24404184
    27. Recruitment of STXBP1 by the Rab27A effector SYTL4 promotes Weibel-Palade body exocytosis. PMID: 24700782
    28. The results showed that Rab27A(Q78L) is unable to localize on mature melanosomes and that its inhibitory activity on melanosome transport is completely dependent on its binding to the Rab27A effector Slac2-a/melanophilin. PMID: 24584932
    29. Exosomes derived from Rab27aoverexpressing cancer cells elicited more potent antitumor immune effects. PMID: 24146068
    30. several crystal structures of the myosin Va or the myosin Vb globular tail domain that gives insights into how the motor is linked to the recycling membrane compartments via Rab11 or the melanophilin adaptor that binds to Rab27a. PMID: 24248336
    31. Rab27a has a key role in eosinophil degranulation. Furthermore, these findings have implications for Rab27a-dependent eosinophil degranulation in airway inflammation. PMID: 23986549
    32. Defects in cargo trafficking caused by mutations in RAB27A and UNC13D genes, encoding Rab27a and its effector Munc13-4, cause severe immunodeficiencies in humans. (Review) PMID: 23810987
    33. Rab27a could improve cell viability, proliferation, and migration of U251 cells and inhibit its apoptosis by promoting secretion of cathepsin D and miR-124 suppression. PMID: 23553027
    34. Upregulation of the Rab27a-dependent lysosomal trafficking and secretory pathways contributes to the correction of some of the cellular defects induced by lysosomal overload in cystinosis, including endoplasmic reticulum stress. PMID: 23716592
    35. Altogether, these results indicate that Rab27a plays an important role in herpes simplex virus 1 infection of oligodendrocytic cells. PMID: 23164453
    36. RAB27A localization and function in melanocytes. [Review] PMID: 23176485
    37. the intrinsically essential role of RAB27A in human ethnic skin color determination PMID: 22844437
    38. Rab27 and Rab3 sequentially regulate human sperm dense-core granule exocytosis, and Rab27 is also required for the acrosome reaction. PMID: 22753498
    39. Upregulated expression of rab4, rab5, rab7, and rab27 correlates with antemortem measures of cognitive decline in individuals with mild cognitve impairment and Alzheimer disease. PMID: 21669283
    40. The pathologic defect in Griscelli syndrome 3 came from the MLPH R35W substitution, which induced aggregation of melanosomes in the perinuclear area of melanocytes because of failure to interact with RAB27A. PMID: 21883982
    41. A Rab27a/MyRIP/myosin Va complex is involved in linking von-Willebrand factor (Vwf) to the peripheral actin cytoskeleton of endothelial cells to allow full maturation and prevent premature secretion of vWF. PMID: 21740491
    42. Data show that point mutations in the binding motif of munc13-4 have severely impaired rab27a binding, allowing dissection of rab27a requirements in munc13-4 function. PMID: 21693760
    43. Rab27a hasa role in the CMV life cycle and CMV and LRO biogenesis share common molecular mechanisms PMID: 21170347
    44. Rab27a plays a direct regulatory role in the nascent process of phagocytosis by prolongation of the stage of actin coating via suppression of Coronin 1A. PMID: 21169636
    45. Molecular analysis revealed a novel homozygous mutation in exon 5, namely, a single-base substitution (g.42996 A>G) leading to an amino acid change (S115G) and thus confirming the diagnosis of Griscelli syndrome type 2. PMID: 21314004
    46. Loss of Rab27a increased the fraction of mobile lytic granules and the extent of their movement in the cytosol. PMID: 20877725
    47. TBC1D16 and RAB27A, were identified known drivers of melanoma both are involved in the regulation of vesicular trafficking, which highlights this process as important for proliferation in melanoma. PMID: 21129771
    48. The Rab27A(K22R) mutant normally binds Munc13-4, but not Slp2-a or Slp4-a, whereas the Rab27A(I44T) mutant shows reduced binding activity to Slp2-a and Munc13-4 but normally binds Slp4-a. PMID: 20370853
    49. RAB27A mutations were found in 1 of the 21 families with haemophagocytic syndromes without mutations in familial HLH (FHL) causing genes PMID: 19953648
    50. role in melanosome transport PMID: 11980908

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  • 相關疾?。?/div>
    Griscelli syndrome 2 (GS2)
  • 亞細胞定位：
    Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome.
  • 蛋白家族：
    Small GTPase superfamily, Rab family
  • 組織特異性：
    Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9766

    OMIM: 603868

    KEGG: hsa:5873

    STRING: 9606.ENSP00000337761

    UniGene: Hs.654978