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RPS19 Recombinant Monoclonal Antibody

  • 中文名稱：
    RPS19重組抗體
  • 貨號(hào)：
    CSB-RA940350A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: HEK293 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, A549 whole cell lysate
      All lanes: RPS19 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 17 kDa
      Observed band size: 15-20 kDa
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA940350A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA940350A0HU RPS19重組單克隆抗體是針對(duì)核糖體蛋白S19（RPS19）研發(fā)的高特異性檢測(cè)工具。該靶標(biāo)蛋白作為40S核糖體亞基的重要組成部分，參與rRNA加工和蛋白質(zhì)生物合成調(diào)控，其功能異常與遺傳性貧血及細(xì)胞周期紊亂密切相關(guān)。本產(chǎn)品經(jīng)嚴(yán)格驗(yàn)證，在Western Blot中可清晰識(shí)別特異性蛋白條帶，推薦使用1:500-1:2000稀釋度；流式細(xì)胞分析（FC）在1:50-1:200稀釋范圍內(nèi)能有效檢測(cè)細(xì)胞內(nèi)源表達(dá)?？贵w采用重組單克隆技術(shù)制備，具有出色的批次穩(wěn)定性和低交叉反應(yīng)性，配套實(shí)驗(yàn)數(shù)據(jù)表明其在非還原性條件下的檢測(cè)性能表現(xiàn)優(yōu)異。適用于探索核糖體組裝機(jī)制、紅細(xì)胞發(fā)育異常相關(guān)研究、以及腫瘤細(xì)胞增殖調(diào)控等基礎(chǔ)科研領(lǐng)域，為蛋白質(zhì)相互作用研究、基因表達(dá)調(diào)控分析及疾病模型構(gòu)建提供可靠工具。
  • Uniprot No.：
  • 基因名：
    RPS19
  • 別名：
    40S ribosomal protein S19 (Small ribosomal subunit protein eS19), RPS19
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human RPS19
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    7E6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for pre-rRNA processing and maturation of 40S ribosomal subunits.
  • 基因功能參考文獻(xiàn)：
    1. Study shows that the depletion of RPS19 causes a reduction of 47S rRNA synthesis in a number of cell lines of different origins. PMID: 27734913
    2. The results show that RPS19, RPS21 or RPS24 are upregulated in malignant tissue and may serve as putative biomarkers for prostate cancer. PMID: 29016636
    3. Study of genetic risk of prevalent hrHPV infections in Nigerian women found significant associations with SNPs on ribosomal protein gene S19 (RPS19) and Thymidylate Synthase gene (TYMS), in an allelic model. This risk remained significant, after adjusting for age, body mass index, smoking, age at menarche, age at sexual debut, lifetime total number of sexual partners and the total number of pregnancies. PMID: 23826176
    4. Reducing RPS19 in tumor cells or blocking the C5a receptor 1-RPS19 interaction decreases RPS19-mediated immunosuppression, impairs tumor growth, and delays the development of tumors in a transgenic model of breast cancer PMID: 28228558
    5. RPS19-downregulated erythroleukemia cells show reduced FLVCR1a and FLVCR1b mRNA levels associated with heme overload. PMID: 26058344
    6. Loss of RPS19 expression is associated with Diamond-Blackfan anemia. PMID: 25216681
    7. Mutations R62W and R101H impair RPS19 ability to associate with the ribosome. PMID: 25069755
    8. Data indicate that GATA1 transcription factor is downregulated in ribosomal protein S19 (RPS19)-deficient cells through upregulation of TNF-alpha and p38 MAPK. PMID: 25270909
    9. RPS19 mutation is associated with Diamond Blackfan Anemia. PMID: 25132370
    10. A binding domain for RPS19 was identified and characterized in the N-terminus. PMID: 25062117
    11. increase of autophagy in cells derived from DBA patients, in CD34+ erythrocyte progenitor cells with RPS19 knock down, in the red blood cells of zebrafish embryos with RP-deficiency, and in cells from patients with Shwachman-Diamond syndrome PMID: 24875531
    12. High frequency of RPS19 gene deletion is associated with Italian Diamond-Blackfan anemia. PMID: 22689679
    13. RPS19 mutations induced a decrease in proliferation of progenitor cells, but the terminal erythroid differentiation was normal with little or no apoptosis. PMID: 22833095
    14. Single nucleotide polymorphisms in the RPS19 and RPS19 is associated with HPV persistence and cervical precancer/cancer. PMID: 22496757
    15. the monocyte C5aR selectively activates the classical pathway with the binding of C5a and the alternative pathway with the binding of C5a/RP S19. PMID: 21613290
    16. The RPS19 gene uses a broad range of transcriptional start sites and a short 5'UTR is associated with increased levels of RPS19. PMID: 21412415
    17. translocation of RP S19 from prothrombin to platelets during blood coagulation PMID: 21306436
    18. N-mediated translation initiation mechanism, which lures the host translation machinery for the preferential translation of viral transcripts, primarily depends on the N-RPS19 interaction. PMID: 21296889
    19. PIM1 may act as a sensor for ribosomal stress independently of or in concert with the known p53-dependent mechanisms. PMID: 20639905
    20. approximately 30% of RPS19 mutations are missense mutations that do not alter the stability of the RPS19 protein and are hypothesized to act by a dominant negative mechanism in Diamond-Blackfan anemia PMID: 20606162
    21. Data show that when a Gi/PI3K pathway is partially blocked, C5a receptors stimulate an alternative p38MAPK pathway. PMID: 20473571
    22. RPS19 binds specifically to the 5' untranslated region of its own mRNA; Diamond-Blackfan anemia missense mutations introduced into RPS19 impair this binding. PMID: 20395159
    23. A molecule indistinguishable from RP S19 was present in plasma, and that the RP S19-like molecule was converted to the active form by a transglutaminase-catalyzed reaction. PMID: 20093496
    24. Genetic variants in the noncoding region of RPS19 gene in Diamond-Blackfan anemia: potential implications for phenotypic heterogeneity. PMID: 20054847
    25. Study suggests that RPS19 overexpression might be a universal signal in rapid cell growth tissues. PMID: 11942409
    26. results indicated that the conversion of the RP S19 dimer from agonist to antagonist of C5a receptor is attributed to the IAGQVAAANKK moiety between Ile134 and Lys144 PMID: 12651630
    27. Histopathological grading of the HNSCC biopsies correlated significantly with the S19 mRNA expression levels. PMID: 15330156
    28. RPS19 silencing decreases the proliferative capacity of hematopoietic progenitors and leads to a defect in erythroid development PMID: 15626736
    29. Deficiency of RPS19 blocks proliferation of immature erythroid progenitor cells; dexamethasone activates proliferation of the same cell population through mechanisms independent of RPS19 PMID: 15755903
    30. Rps19 has a role in the assembly and maturation of the pre-40 S ribosomal particles PMID: 16159874
    31. The PIM-1/RPS19 interaction was demonstrated both in vitro and in living cells and led to phosphorylation of RPS19 in an in vitro kinase assay. PMID: 16266891
    32. in CD34- cells from the bone marrow of patients with DBA harboring mutations in RPS19 was revealed a pre-rRNA-processing defect similar to that observed in TF-1 cells where RPS19 expression was reduced PMID: 16990592
    33. ribosome biogenesis and nucleolar organization is altered in skin fibroblasts from DBA patients bearing mutations in the RPS19 gene PMID: 17053056
    34. RPS19 mutations affecting RPS19 conserved arginines R56Q and R62Q could significantly inhibit the rate of protein synthesis, indicating the importance of RPS19 in translation. PMID: 17082006
    35. RPS19 is a ribosomal protein linked to Diamond-Blackfan anemia PMID: 17178250
    36. The RP S19 dimer inhibits C5a-induced neutrophil migration and promotes apoptosis of neutrophils via the C5a receptor. PMID: 17199736
    37. Diamond-Blackfan anemia missense mutations affect the assembly of ribosomal protein S19 into the ribosome PMID: 17517689
    38. RPS19 deficiency causes apoptosis and accelerated loss of erythroid progenitors in RPS19-deficient Diamond-Blackfan anemia. PMID: 17962699
    39. patients with RPS19 mutations display a poorer response to steroids and a worse long-term prognosis compared to other Diamond-Blackfan anemia patients (Review) PMID: 18412286
    40. Demonstration of an important role for the proteasomal degradation pathway in regulating the expression levels and nucleolar localization of certain mutant RPS19 proteins in Diamond-Blackfan anemia. PMID: 18768533
    41. RPS19 significantly compromised CXCR2-dependent MIF-triggered adhesion of monocytes to endothelial cells under flow conditions. We, therefore, propose that RPS19 acts as an extracellular negative regulator of MIF. PMID: 19155217
    42. siRNA knock-down of RPS19 results in a relative decrease of small subunit r-proteins (S20, S21 and S24) when compared to large subunit r-proteins (L3, L9, L30 and L38). This correlates with a relative decrease in 18S rRNA with respect to 28S rRNA. PMID: 19454283
    43. Specific alleles at predicted transcription factor binding sites may alter the expression of RPS19, modify an important interaction between transcription factors with overlapping TFBS or remove an important stimulus for hematopoiesis PMID: 19587786
    44. Primary fibroblasts from Diamond-Blackfan anemia patients with truncating mutations in RPS19 or in RPS24 have a marked reduction in proliferative capacity. PMID: 19689926

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  • 相關(guān)疾?。?/div>
    Diamond-Blackfan anemia 1 (DBA1)
  • 亞細(xì)胞定位：
    Nucleus. Note=Located more specifically in the nucleoli.
  • 蛋白家族：
    Eukaryotic ribosomal protein eS19 family
  • 組織特異性：
    Higher level expression is seen in the colon carcinoma tissue than normal colon tissue.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 10402

    OMIM: 105650

    KEGG: hsa:6223

    STRING: 9606.ENSP00000470004

    UniGene: Hs.438429