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TBPL1 Recombinant Monoclonal Antibody

  • 中文名稱：
    TBPL1 Recombinant Monoclonal Antibody
  • 貨號(hào)：
    CSB-RA297595A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate(20μg), HepG2 whole cell lysate(20μg), U251MG whole cell lysate(20μg), SH-SY5Y whole cell lysate(20μg), Hela whole cell lysate(20μg), 293T whole cell lysate(20μg)
      All lanes: TBPL1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 21 kDa
      Observed band size: 23 kDa
      Exposure time：2min
    • IHC image of CSB-RA297595A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA297595A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of HepG2 cell with CSB-RA297595A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA297595A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    TBPL1
  • 別名：
    21 kDa TBP-like protein antibody; 21kDa TBP like protein antibody; Second TBP of unique DNA antibody; Second TBP of unique DNA protein antibody; STUD antibody; TATA box binding protein like protein 1 antibody; TATA box binding protein-related factor 2 antibody; TATA box-binding protein-like protein 1 antibody; TATA box-binding protein-related factor 2 antibody; TBP like 1 antibody; TBP like protein antibody; TBP related factor 2 antibody; TBP-like factor antibody; TBP-like protein 1 antibody; TBP-related factor 2 antibody; TBP-related protein antibody; tbpl1 antibody; TBPL1_HUMAN antibody; TLF antibody; TLP antibody; TRF2 antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human TBPL1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    5G7
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.
  • 基因功能參考文獻(xiàn)：
    1. TLP Prevents p53 Degradation through Disrupting the p53-MDM2 Interaction. PMID: 28082682
    2. The interaction of TBP-like protein with TFIIA is essential to suppress proteasome-dependent turnover of TBP-like protein PMID: 27696626
    3. miR18a exhibits a protective role in colorectal cancer (CRC) via inhibiting proliferation, invasion and migration of CRC cells by directly targeting the TBPL1 gene. PMID: 26398009
    4. results suggest that activation of the p21 upstream promoter is mediated by interaction between specific regions of TLP and p53 PMID: 24594805
    5. Western blotting and immunohistochemistry both indicated a higher TBPL1 protein expression level in colorectal cancer tissue. siRNA for TBPL1 transfection obviously slowed down the cell proliferation in both SW-620 and HT-29 cells. PMID: 24870791
    6. TLP mediates p53-governed transcriptional activation of the p21 upstream promoter. PMID: 22511763
    7. Data show that during interphase, endogenous or exogenously expressed TRF2 is located almost exclusively in the nucleolus PMID: 15269281
    8. TLF is a functional regulator of transcription with targets distinct from those of TBP. PMID: 15767669

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    TBP family
  • 組織特異性：
    Ubiquitously expressed, with highest levels in the testis and ovary.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 11589

    OMIM: 605521

    KEGG: hsa:9519

    STRING: 9606.ENSP00000237264

    UniGene: Hs.486507