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USP24 Recombinant Monoclonal Antibody

  • 中文名稱：
    USP24重組抗體
  • 貨號(hào)：
    CSB-RA178866A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Immunofluorescence staining of HepG2 cell with CSB-RA178866A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 569-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing HepG2 cells stained with CSB-RA178866A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA178866A0HU USP24重組單克隆抗體是針對(duì)泛素特異性蛋白酶24（USP24）研發(fā)的高特異性科研工具。USP24是去泛素化酶家族成員，通過調(diào)控靶蛋白穩(wěn)定性參與DNA損傷應(yīng)答、細(xì)胞周期調(diào)控及凋亡等過程，其異常表達(dá)與腫瘤發(fā)生、神經(jīng)退行性疾病等病理機(jī)制相關(guān)。本抗體經(jīng)ELISA、免疫熒光（IF）和流式細(xì)胞術(shù)（FC）嚴(yán)格驗(yàn)證，在IF實(shí)驗(yàn)中可清晰呈現(xiàn)細(xì)胞內(nèi)USP24的亞定位分布，F(xiàn)C分析能準(zhǔn)確檢測(cè)蛋白表達(dá)水平，推薦使用稀釋度均為1:50-1:200。其高親和力與低交叉反應(yīng)性設(shè)計(jì)確保實(shí)驗(yàn)數(shù)據(jù)的可靠性，適用于泛素化修飾調(diào)控機(jī)制研究、疾病相關(guān)蛋白降解通路探索及藥物靶點(diǎn)篩選等科研場(chǎng)景，為體外細(xì)胞模型或組織樣本中USP24的功能研究提供穩(wěn)定支持。該產(chǎn)品適用于基礎(chǔ)分子生物學(xué)研究與疾病機(jī)制解析，不涉及其他用途。
  • Uniprot No.：
  • 基因名：
    USP24
  • 別名：
    Ubiquitin carboxyl-terminal hydrolase 24 (EC 3.4.19.12) (Deubiquitinating enzyme 24) (Ubiquitin thioesterase 24) (Ubiquitin-specific-processing protease 24), USP24, KIAA1057
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human USP24
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    11E3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ubiquitin-specific protease that regulates cell survival in various contexts through modulating the protein stability of some of its substrates including DDB2, MCL1 or TP53. Plays a positive role on ferritinophagy where ferritin is degraded in lysosomes and releases free iron.
  • 基因功能參考文獻(xiàn)：
    1. USP24 level was decreased during the early stage of cancer and the mitotic stage of the cell cycle to regulate its substrates p300, Bax, E2F4 and securin, resulting in decreased cell apoptosis and increased cell cycle progression and, thus, cancer formation. PMID: 27991932
    2. Data show that ubiquitin-specific peptidase 24 (USP24) is highly expressed in cell lines with enhanced malignancy and in late-stage lung cancer clinical samples. PMID: 26568301
    3. USP24 deubiquitinase regulates the DNA damage response by directly targeting the p53 tumor suppressor. PMID: 25578727
    4. the novel compound EOAI3402143 dose-dependently inhibited Usp9x and Usp24 activity, increased tumor cell apoptosis, and fully blocked or regressed myeloma tumors in mice. PMID: 25814533
    5. These results suggested that USP24 expression is tightly regulated at its transcription level and NFkappaB plays an important role in this process. PMID: 24286619
    6. The knockdown of USP24 in two human cell lines decreased the steady-state levels of DDB2, indicating that USP24-mediated DDB2 deubiquitination prevents DDB2 degradation. PMID: 23159851
    7. The present study is the first to report a lack of association between SNPs of USP24 and parkinson disease in Han Chinese patients. Other PMID: 22923019
    8. USP24 alone plays a role in PD susceptibility among Taiwanese people >or=60 years of age, or acting synergistically with USP40 and UCHL1 in the total subjects. PMID: 20302855
    9. Data suggest that genetic variants in USP24 affect the risk for late-onset Parkinson disease (PD), which is consistent with the predicted role of the ubiquitination pathway in PD etiology. PMID: 16917932

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  • 蛋白家族：
    Peptidase C19 family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12623

    OMIM: 610569

    KEGG: hsa:23358

    STRING: 9606.ENSP00000294383

    UniGene: Hs.477009