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  4. Acetyl-Histone H2B type 1-B (K20) Recombinant Monoclonal Antibody

Acetyl-Histone H2B type 1-B (K20) Recombinant Monoclonal Antibody

  • 中文名稱：
    乙酰-組蛋白 H2B type 1-B (K20)重組抗體
  • 貨號：
    CSB-RA010402A20acHU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30min
      All lanes Acetyl-Histone H2B type 1-B(K20)antibody at 0.135μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 15 KDa
      Observed band size: 15 KDa
    • Immunocytochemistry analysis of CSB-RA010402A20acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010402A20acHU at 1:84,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
    • Overlay histogram showing Hela cells stained with CSB-RA010402A20acHU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA010402A20acHU是針對組蛋白H2B第20位賴氨酸乙酰化修飾（Acetyl-Histone H2B type 1-B K20）開發(fā)的重組單克隆抗體。組蛋白H2B作為核小體核心成分，其K20位點的乙?；揎椩诒碛^遺傳調(diào)控中發(fā)揮重要作用，參與染色質(zhì)結(jié)構(gòu)重塑、轉(zhuǎn)錄激活及DNA損傷修復等生物學過程。本產(chǎn)品經(jīng)過ELISA、Western Blot（WB）、免疫細胞化學（ICC）、免疫熒光（IF）及流式細胞術(shù)（FC）等多平臺驗證，在WB實驗中表現(xiàn)出高特異性，推薦使用1:5000-1:10000稀釋比例可清晰檢測目標條帶；ICC/IF應用推薦1:50-1:500及1:30-1:200稀釋范圍，適用于細胞定位分析，能精準顯示核內(nèi)乙酰化修飾信號；FC檢測則支持細胞群體表觀遺傳狀態(tài)分析。該抗體適用于染色質(zhì)動態(tài)研究、組蛋白翻譯后修飾機制探索，以及發(fā)育生物學、腫瘤微環(huán)境等領(lǐng)域中表觀遺傳調(diào)控網(wǎng)絡的科研工作，為探索基因表達調(diào)控、干細胞多能性維持及疾病相關(guān)信號通路提供可靠工具。
  • Uniprot No.：
  • 基因名：
    HIST1H2BB
  • 別名：
    HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    21F11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, ICC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:5000-1:10000
    ICC 1:50-1:500
    IF 1:30-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 基因功能參考文獻：
    1. Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants has been uncovered. PMID: 29643390
    2. RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response. PMID: 26854224
    3. RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling PMID: 21362548
  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2B family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 4751

    OMIM: 602803

    KEGG: hsa:3018

    STRING: 9606.ENSP00000350580

    UniGene: Hs.553494