COMT Recombinant Monoclonal Antibody
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中文名稱:COMT重組抗體
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貨號(hào):CSB-RA909081A0HU
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規(guī)格:¥1320
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圖片:
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Western Blot
Positive WB detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate
All lanes: COMT Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31, 25 kDa
Observed band size: 31 kDa -
IHC image of CSB-RA909081A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:COMT Recombinant Monoclonal Antibody(CSB-RA909081A0HU)是針對兒茶酚-O-甲基轉(zhuǎn)移酶(COMT)開發(fā)的高特異性抗體,適用于多種實(shí)驗(yàn)場景。COMT是催化兒茶酚胺類神經(jīng)遞質(zhì)甲基化代謝的關(guān)鍵酶,廣泛參與多巴胺、腎上腺素等生物活性分子的調(diào)控,其功能異常與神經(jīng)系統(tǒng)疾病及代謝紊亂密切相關(guān)。本抗體經(jīng)嚴(yán)格驗(yàn)證,可在ELISA、Western Blot(WB)和免疫組化(IHC)中穩(wěn)定檢測內(nèi)源性COMT蛋白表達(dá)。在WB實(shí)驗(yàn)中,該抗體表現(xiàn)出高靈敏度,推薦使用稀釋比例為1:500至1:5000,可清晰識(shí)別目標(biāo)條帶;在IHC應(yīng)用中,1:50-1:200的稀釋范圍能有效定位COMT在組織切片中的亞細(xì)胞分布,顯色信號(hào)明確且背景低。其重組單克隆特性確保了批次間的高度一致性,適用于神經(jīng)生物學(xué)研究、疾病機(jī)制探索、蛋白表達(dá)水平分析及組織定位實(shí)驗(yàn),為研究COMT在神經(jīng)遞質(zhì)代謝、藥物反應(yīng)或相關(guān)信號(hào)通路中的功能提供可靠工具。本產(chǎn)品僅限科學(xué)研究使用。
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Uniprot No.:
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基因名:
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別名:Catechol O-methyltransferase (EC 2.1.1.6), COMT
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反應(yīng)種屬:Human
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免疫原:A synthesized peptide derived from human COMT
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免疫原種屬:Homo sapiens (Human)
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標(biāo)記方式:Non-conjugated
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克隆類型:Monoclonal
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抗體亞型:Rabbit IgG
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純化方式:Affinity-chromatography
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克隆號(hào):8E1
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濃度:It differs from different batches. Please contact us to confirm it.
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保存緩沖液:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式:Liquid
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應(yīng)用范圍:ELISA, WB, IHC
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推薦稀釋比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 -
Protocols:
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儲(chǔ)存條件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途:For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻(xiàn)
- Effects of latroeggtoxin-VI on dopamine and α-synuclein in PC12 cells and the implications for Parkinson's disease D Yu,Biological research,2024
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能:Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
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基因功能參考文獻(xiàn):
- There was no significant genotypic association between rs4680 and clinical symptoms or cognitive function in Han Chinese patients with schizophrenia. Patients with GG at rs165599 scored significantly higher on the Stroop test, suggesting better cognitive performance after 8 weeks of treatment. The findings suggest that the COMT gene polymorphisms may influence the response to antipsychotic treatment. PMID: 29634613
- These findings suggest that the association between COMT polymorphisms and cognitive functioning could be, at least in part, due to their association with varying levels of S-COMT. This is important as, unlike MB-COMT, the substrates targeted by S-COMT are likely to be intra-cellular rather than, like dopamine, located mainly in the synaptic vesicles or the extra-cellular space. PMID: 30218069
- The COMT gene rs165599 SNP does not appear to be a single-risk factor for schizophrenia. PMID: 30165727
- An interaction between the COMT genotype and childhood adversity, affects executive function in adolescents. PMID: 29222971
- that the presence of one or two Met alleles of the COMT Val(158/108)Met might act as a protective variant in working memory tasks in combat exposed veterans with posttraumatic stress disorder PMID: 29248614
- Association between COMT Val158Met polymorphisms with antisaccade task performance in schizophrenic patients. PMID: 29429137
- variant and temporomandibular disorders may contribute to individual variation in electric and cold pulp sensitivity PMID: 29550002
- T-77C and Arg399Gln polymorphisms of the XRCC1 gene, as well as the 186C>T and Val158Met polymorphisms of the COMT gene, increased the risk of lung cancer in non-smoking women. PMID: 30109864
- COMT-GG and NRG1-AA genotypes aid the transcranial direct current stimulation-induced improvement in auditory verbal hallucinations in schizophrenia patients. PMID: 29559020
- In those who had used cannabis before 20 years of age, COMT Val158Met polymorphism had a trend level effect on age of onset of psychosis (median age of onset of psychosis : Val/Val < Val/Met < Met/Met 19.37, 20.95, 21.24 years, respectively; log-rank test p = .051). PMID: 29201551
- findings support the hypothesis that G x E interactions underlie associations of COMT val158met with fear inhibition deficits PMID: 28833952
- COMT gene moderates the relation between maternal history of maltreatment and infant emotion regulation. PMID: 28803562
- Its single-nucleotide polymorphisms involves in dopaminergic metabolism and motor and cognitive function in older adults. PMID: 29525179
- COMT DNA methylation level is affected by the severity of the clinical symptoms of schizophrenia and might also be influenced by pharmacological treatment. PMID: 29160620
- The present study showed that females with middle dopamine availability (valA/valB or met/met) and evidence of emotional problems in adolescence were more likely to have affective symptoms at age 53. PMID: 29331705
- findings suggest that Val/Val homozygous individuals for catachol-O-methyltransferase (COMT) may be more flexible regarding self-attribution/body ownership and that biological factors may contribute to reduced awareness regarding the distinction between self and others. PMID: 28251370
- Cloninger's (1987) hypothesis about negative relationship between novelty seeking and dopamine was confirmed on allele level, because higher novelty seeking was found in Val allele carriers comparing to Met/Met genotype carriers. PMID: 29546858
- Study provides evidence for an epistatic interaction between ZNF804A rs1344706 and COMT rs4680 on the grey matter volume of the left dorsolateral prefrontal cortex, which may improve our understanding on the biological mechanism underlying the genome-wide supported variant ZNF804 rs1344706 of schizophrenia on the prefrontal anatomy. PMID: 28078547
- Logistic regressions with interaction terms (adjusted for sex and age) revealed that comt rs4680 interacted with total childhood adversity, emotional abuse and physical abuse in predicting panic disorder. PMID: 29574383
- No significant difference was observed in the RNA levels of CYP1A1 and SULT1A1 between the two groups. The frequency of expression of the CYP17 T/C variant tended to be higher and the A allele of COMT polymorphism together with down-regulation of its mRNA expression may be more frequent in Chinese women with idiopathic POI PMID: 28887105
- Individuals carrying Val/Val of COMT seem to be more sensitive to the synergistic effect of environmental factors acting early in neurodevelopment. PMID: 28262436
- childhood urbanicity and variation in dopamine genes COMT, DRD1 and DRD2 alters adult prefrontal function as measured by fMRI PMID: 29634738
- None of the polymorphisms studied showed a reliable association with response inhibition performance. The methodological and theoretical implications of these findings are discussed. PMID: 29155230
- the association between the COMT Val158Met and ZDHHC8 rs175174 single nucleotide polymorphism and the susceptibility to schizophrenia through a case-control study involving a population from the North Region of Brazil, was investigated. PMID: 28562378
- Results revealed a sex-specific effect of COMT on corpus callosum (CC): in males only, Val homozygotes had significantly higher fractional anisotropy (FA) compared to Met-carriers. Volume-of-interest analysis showed a genotype by sex interaction on FA in genu and rostral midbody of CC, whereby Val males demonstrated higher FA than Met females. PMID: 28948081
- No association between self-reported non-recovery or pain levels and COMT haplotypes in in a Northern European patients with acute whiplash injuries could be detected. PMID: 29195501
- The aim of this study was to investigate the effects of the interaction between the CYP 2A6 and COMT genes on smoking behavior in young Taiwanese men. The odds ratio for starting smoking was significantly lower in subjects carrying a CYP2A6 low activity/variant COMT rs4680 genotype than in those possessing a CYP2A6 wild-type/variant COMT rs4680 genotype (0.44, 95% confidence interval = 0.19-0.98, P = 0.043). PMID: 28472995
- Results show no association between TPMT or COMT single-nucleotide polymorphisms and cisplatin-induced ototoxicity. PMID: 28445188
- Study applied graph theory analysis on resting-state fMRI of 120 women selected based on neuroticism score, & genotyped 2 polymorphisms: 5-HTTLPR (S-carriers and L-homozygotes) and COMT (rs4680-rs165599; COMT risk group and COMT non-risk group). The COMT polymorphism moderated the association between neuroticism and functional network organization. PMID: 27743374
- The purpose of the current study is to test the interaction between prenatal maternal smoking and COMT Val(158)Met in conduct problems and crime in the 1993 Pelotas Birth Cohort Study. PMID: 27426045
- We found no modulation of saccade parameters by COMT genotype in a visually guided control task. PMID: 27793591
- Study used a mediation analysis to identify a gene-anatomy-cognition pathway to explain how COMT Val158Met polymorphism affects interference resolution capacity via modulating the gray matter volume of the left lateral frontal pole in healthy male subjects. PMID: 27664968
- Findings do not suggest an additional impairment from comorbid alcohol and substance use disorders (AUDs/SUDs) on facial emotion recognition among subjects with schizophrenia, whilst COMT Val158Met, though based on a limited sample, might have a role just among those without AUDs/SUDs. PMID: 28913946
- In a study of the genetic association between polymorphisms in the DAT1, SERT, COMT and BDNF genes and attention deficit and hyperactive disorder, transmission disequilibrium test analysis showed that no individual allele of any variant studied has a preferential transmission PMID: 29122229
- There is an association between COMT Val108/158Met and schizophrenia in the general population (Review and Meta-analysis). PMID: 27020768
- Val/Met polymorphism as a candidate gene variant to assess associations with subjective dimensions of hypnosis PMID: 28836919
- A significant difference in the genotype distribution of the COMT A158G polymorphism was demonstrated, favoring the low activity genotype in patients with Polyneuropathy compared to both controls and patients without Polyneuropathy. PMID: 28759974
- The present results indicate that rs4633 and rs4680 catechol-O-methyltransferase polymorphisms influence cerebrospinal fluid dopamine concentrations and methamphetamine toxicity in MA abusers. PMID: 27987399
- The COMT variant (rs4680) was associated with poor appetite/undereating in an adolescent population. PMID: 28694222
- The findings showed that a group of patients with a GG single nucleotide polymorphism (SNP) rs4680 in COMT required a significantly higher dose of morphine than a non-GG group. PMID: 28985716
- For case-only studies, a significant interaction was found between cannabis use and COMTVal158Met, with an OR of 1.45 (95% Confidence Interval = 1.05-2.00; Met/Met as the risk genotype (Meta-Analysis) PMID: 29444152
- To determine the frequency of COMT Val158Met polymorphism. PMID: 28968204
- Analyses suggest that COMT val158met polymorphism moderates the influence of early life stress on preschool-age symptoms of anxiety. PMID: 28859863
- Our study demonstrated nonlinear modulation of the interaction between COMT and depression on brain function. These findings expand our understanding of the COMT effect underlying the pathophysiology of MDD. PMID: 28728097
- there is a relationship between Val158Met COMT and certain social cognitive deficits in patients with schizophrenia PMID: 28856668
- meta-analysis suggested that the COMT Val158Met polymorphism was not associated with migraine risk (Meta-Analysis) PMID: 27154995
- These findings support the hypothesis of a broad effect of the Val158Met polymorphism in the COMT gene on several dimensions of behavior and neuropsychiatric symptoms. PMID: 28235603
- It is unlikely that the investigated genetic variants are clinically relevantly associated with depression after diagnosis of cancer. PMID: 28590587
- In our study, statistical analysis has showed that in control group Val/Met COMT genotype was significantly higher compared with the obesity group PMID: 28629779
- COMT 158G/A (COMT Val158Met) polymorphism was associated with suicide susceptibility only in females. PMID: 28676589
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相關(guān)疾?。?/div>Schizophrenia (SCZD)亞細(xì)胞定位:[Isoform Soluble]: Cytoplasm.; [Isoform Membrane-bound]: Cell membrane; Single-pass type II membrane protein; Extracellular side.蛋白家族:Class I-like SAM-binding methyltransferase superfamily, Cation-dependent O-methyltransferase family組織特異性:Brain, liver, placenta, lymphocytes and erythrocytes.數(shù)據(jù)庫鏈接:
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