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  4. FEN1 Recombinant Monoclonal Antibody

FEN1 Recombinant Monoclonal Antibody

  • 中文名稱：
    FEN1重組抗體
  • 貨號：
    CSB-RA008585A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
      All lanes: FEN1 antibody at 0.775μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 43, 36 KDa
      Observed band size: 43 KDa
    • IHC image of CSB-RA008585A0HU diluted at 1:77.5 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-RA008585A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA008585A0HU FEN1重組單克隆抗體是針對DNA修復(fù)關(guān)鍵酶FEN1（Flap Endonuclease 1）開發(fā)的高特異性科研工具。該抗體靶向的FEN1蛋白在DNA復(fù)制與修復(fù)中發(fā)揮核心作用，通過精確切割5'端懸垂結(jié)構(gòu)參與岡崎片段成熟及堿基切除修復(fù)，對維持基因組穩(wěn)定性至關(guān)重要。本品經(jīng)ELISA、蛋白質(zhì)印跡（WB）、免疫組化（IHC）和免疫熒光（IF）多平臺驗(yàn)證，推薦使用濃度分別為WB 1:500-1:5000、IHC 1:50-1:200、IF 1:20-1:200，能有效識別天然構(gòu)象的FEN1蛋白。實(shí)驗(yàn)數(shù)據(jù)顯示其在不同物種來源的細(xì)胞裂解液及石蠟包埋組織中均呈現(xiàn)優(yōu)異特異性，批間穩(wěn)定性良好，適用于探究DNA損傷應(yīng)答機(jī)制、細(xì)胞周期調(diào)控及腫瘤發(fā)生相關(guān)研究。該重組抗體采用哺乳動物表達(dá)系統(tǒng)制備，具有高親和力和低交叉反應(yīng)性特點(diǎn)，可為DNA代謝研究、基因組穩(wěn)定性分析以及相關(guān)分子通路探索提供可靠檢測工具，滿足蛋白質(zhì)定位、表達(dá)水平檢測等基礎(chǔ)科研需求。
  • Uniprot No.：
  • 基因名：
  • 別名：
    DNase IV antibody; FEN-1 antibody; FEN1 antibody; FEN1_HUMAN antibody; Flap endonuclease 1 antibody; Flap structure specific endonuclease 1 antibody; Flap structure-specific endonuclease 1 antibody; hFEN-1 antibody; hFEN1 antibody; Maturation factor 1 antibody; MF1 antibody; Rad2 antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human FEN1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    4D9
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
  • 基因功能參考文獻(xiàn)：
    1. Results provide evidence that FEN1 rs174538 and rs4246215 are significantly associated with Wilms tumor susceptibility. PMID: 29937070
    2. FEN1 was an independent predictor of survival in pancreatic ductal adenocarcinoma PMID: 29735403
    3. The FEN1 rs174538 A allele is a novel protective biomarker for endometriosis PMID: 29109095
    4. This study revealed that cell apoptosis dysfunction plays a key role in the pathogenesis of LN, even though the difference in FEN1 gene 61563142-61563342 between LN patients and healthy individuals was not statistically significant. PMID: 29239254
    5. Results suggest that FEN1 polymorphisms may reduce the risk of breast cancer in Chinese women. PMID: 27801669
    6. FEN1 is essential for prolifera- tion and cisplatin resistance of lung cancer cells. PMID: 28371273
    7. Our results suggest that functional polymorphism FEN1 rs174538 G>A might affect personal susceptibility to esophageal squamous cell carcinoma . This result provides a solid theoretical foundation for further clinical study using larger sample sizes. PMID: 28319330
    8. FEN1 sculpts DNA with diffusion-limited kinetics to test DNA substrate. This DNA distortion mutually 'locks' protein and DNA conformation and enables substrate verification with extreme precision. PMID: 28230529
    9. Notably, non-small cell lung cancer patients with FEN1-overexpressed cancers were prone to have poor differentiation and poor prognosis. Furthermore, knockdown of FEN1 resulted in G1/S or G2/M phase cell cycle arrest and suppressed in vitro cellular proliferation in NSCLC cancer cells. PMID: 29037854
    10. Data indicate that human cancer-associated genetic alterations in the FEN1 gene can contribute substantially to cancer development. PMID: 27270424
    11. WRN or the Bloom syndrome helicase (BLM) stimulates DNA polymerase delta progression across telomeric G-rich repeats, only WRN promotes sequential strand displacement synthesis and FEN1 cleavage. PMID: 27849570
    12. The FEN1 rs174538 A allele is a protective biomarker for childhood ALL and this association is more significant in males and in patients at onset age of 3.5 years or older. PMID: 26708601
    13. Data indicate that Flap endonuclease 1 (FEN1) single nucleotide polymorphisms and haplotypes are associated with gallbladder cancer risk. PMID: 26668074
    14. the aim of the present study was to determine the role of FEN1 in the chemosensitivity of SGC7901 cells. PMID: 26718738
    15. Binding of DNA to human FEN1 in a bent conformation occurred independently of 5'-flap accommodation and did not require active site metal ions or the presence of conserved active site residues. PMID: 26884332
    16. In this study, we found that cohesion establishment factors, like CHlR1, cooperatively stimulate endonuclease activity of hFen1 in in vivo mimic condition, including replication protein-A-coated DNA and high salt. PMID: 26032365
    17. Under DNA repair conditions, FEN1 efficiently repaired the 5'-phoshotyrosyl bond-containing SSB substrates in the presence of DNA ligase and DNA polymerase. PMID: 26581212
    18. WDR4 regulates FEN1's potential DNA cleavage threat near the site of replication. PMID: 26751069
    19. Patients with expression of both FEN1 and RAD54B were prone to have advanced nodal involvement and significantly poor prognosis. PMID: 26431531
    20. Fen1 significantly stimulated trinucleotide repeats expansion by Pol beta, but not by the related enzyme Pol lambda. PMID: 25687118
    21. YY1 is a transcription repressor of FEN1 regulating FEN1 levels in response to DNA damaging agents. PMID: 25885449
    22. EXO1 and FEN1 cleaved the substrate at the boundary between the single-stranded 5' flap and the duplex, whereas FAN1 incised it three to four nucleotides in the double-stranded region. PMID: 26221031
    23. Rate-determining step of FEN1 reflects a kinetic bias against long flaps and trinucleotide repeat sequences. PMID: 26160176
    24. FEN1 -69G>A and 4150G>T polymorphisms may be associated with cancer susceptibility in Chinese population. PMID: 25154853
    25. Because FEN1 mutations have been identified in human cancers, our findings raise the possibility that unresolved RNA:DNA hybrid structures contribute to the genomic instability associated with cancer PMID: 25922071
    26. A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells. PMID: 25879709
    27. FEN1 protein expression was evaluated in 568 oestrogen receptor (ER) negative breast cancers, 894 ER positive breast cancers and 156 ovarian epithelial cancers. FEN1 is a promising biomarker in breast and ovarian epithelial cancer. PMID: 24880630
    28. The FEN1 E359K germline mutation disrupts the FEN1-WRN interaction and FEN1 GEN activity, causing aneuploidy-associated cancers. PMID: 24608430
    29. the mutations of the FEN1 gene maybe a rare event in the development of hepatocellular carcinomas in Korean population PMID: 24164220
    30. FEN1 SNPs and haplotypes were significantly associated with breast cancer risk. The risk alleles showed decreased FEN1 mRNA expression in breast tissues. PMID: 24440783
    31. The homotrimeric proliferating cell nuclear antigen protein (PCNA) coordinates the actions of DNA polymerase, FEN1 and DNA ligase by facilitating the hand-off intermediates between each protein during Okazaki fragment maturation PMID: 22918592
    32. Conserved basic amino acids are not required for double nucleotide unpairing and appear to act cooperatively, whereas the helical cap plays an unexpected role in hFEN1-substrate rearrangement PMID: 24126913
    33. The data provides the first observations of unpaired conformations in DNA substrates bound to hFEN1 in the presence of catalytically inert active-site metal ions and define the role of amino acid residues and divalent metal ion cofactors in this process. PMID: 23975198
    34. A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-Loop binding properties. PMID: 23675412
    35. This study suggested that FEN1 polymorphisms and haplotypes are associated with glioma risk. PMID: 23184144
    36. We identified small-molecule inhibitors of one such target, FEN1, and showed that these compounds were able to selectively kill human cells carrying cancer-relevant mutations. PMID: 23382697
    37. These studies demonstrate a novel role for APC in the suppression of Fen1 activity in the BER pathway PMID: 22787431
    38. Cell-cycle-dependent timing of FEN1 nuclease activity is essential for cell-cycle progression and the maintenance of genome stability. PMID: 22749529
    39. The crystal structure of the complex of importin alpha with a peptide corresponding to the FEN1 nuclear localization sequence was solved. PMID: 22751659
    40. Review: identifies superfamily motifs such as the helical gateway that select for ss-dsDNA junctions and provides key biological insights into FEN1 specificity and regulation. PMID: 22244820
    41. Data show models for the ternary PCNA/FEN1/DNA and Rad9-Rad1-Hus1 (9-1-1 complex)/FEN1/DNA assemblies. PMID: 22586102
    42. Data show that among the 13 SNPs in the 3 genes, only 3 were found to be polymorphic: R196K and K277R in the DFFB gene, and S12L in the EndoG gene, and all 6 SNPs in the FEN-1 gene were entirely monoallelic. PMID: 22011247
    43. FEN1 as an important gene in human gastrointestinal oncogenesis and genetic polymorphisms in FEN1 confer susceptibility to gastrointestinal cancers. PMID: 22072618
    44. Activity of FEN1 endonuclease on nucleosome substrates is dependent upon DNA sequence but not flap orientation. PMID: 21454907
    45. Structural and functional analyses of human FEN1:DNA complexes show structure-specific, sequence-independent recognition for nicked dsDNA bent 100 degrees with unpaired 3' and 5' flaps. PMID: 21496641
    46. these results show that FEN1 and telomerase association occurs throughout the S phase, with the maximum association in the mid S phase. PMID: 21345332
    47. FEN1 10154T variant allele decreases arachidonic acid and arachidonic acid/linoleic acid in the serum phospholipids of carriers of the APOA5-1131C allele PMID: 20802161
    48. the methylated form, but not the phosphorylated form, of FEN1 strongly interacts with proliferating cell nuclear antigen (PCNA), ensuring the 'on' and 'off' timing of its reaction PMID: 20729856
    49. FEN1 and EXO1 can eliminate structures formed by trinucleotide repeats in the course of replication, relying on endonucleolytic and exonucleolytic activities, respectively. PMID: 20643645
    50. FEN1 maintains stable telomeres by facilitating replication through the G-rich lagging strand telomere, thereby ensuring high fidelity telomere replication. PMID: 20551483

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  • 亞細(xì)胞定位：
    [Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion.
  • 蛋白家族：
    XPG/RAD2 endonuclease family, FEN1 subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 3650

    OMIM: 600393

    KEGG: hsa:2237

    STRING: 9606.ENSP00000305480

    UniGene: Hs.409065