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GRB2 Recombinant Monoclonal Antibody

  • 中文名稱：
    GRB2重組抗體
  • 貨號(hào)：
    CSB-RA981267A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate
      All lanes: GRB2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 26, 21 kDa
      Observed band size: 28 kDa
    • IHC image of CSB-RA981267A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA981267A0HU人源GRB2重組單克隆抗體是針對(duì)生長(zhǎng)因子受體結(jié)合蛋白2（GRB2）研發(fā)的高特異性檢測(cè)工具。該抗體靶向的GRB2蛋白作為重要的信號(hào)轉(zhuǎn)導(dǎo)銜接分子，通過(guò)SH2/SH3結(jié)構(gòu)域介導(dǎo)EGFR等受體酪氨酸激酶與下游RAS-MAPK、PI3K-AKT信號(hào)通路的連接，在細(xì)胞增殖、分化及腫瘤發(fā)生等生物學(xué)過(guò)程中發(fā)揮關(guān)鍵作用。經(jīng)嚴(yán)格驗(yàn)證，本抗體在Western Blot實(shí)驗(yàn)中可特異性識(shí)別內(nèi)源性GRB2蛋白，呈現(xiàn)清晰單一的檢測(cè)條帶，推薦使用濃度范圍為1:500至1:5000；在免疫組化應(yīng)用中能準(zhǔn)確顯示組織樣本中GRB2的亞細(xì)胞定位，建議工作濃度為1:50-1:200。實(shí)驗(yàn)數(shù)據(jù)證實(shí)其在不同樣本類型（包括細(xì)胞裂解液、組織勻漿液及石蠟切片）中均表現(xiàn)出優(yōu)異的批間一致性和重復(fù)性。該產(chǎn)品適用于腫瘤生物學(xué)研究、生長(zhǎng)因子信號(hào)通路解析、受體復(fù)合物互作分析等科研領(lǐng)域，為探索癌癥發(fā)生機(jī)制、細(xì)胞信號(hào)網(wǎng)絡(luò)調(diào)控及蛋白質(zhì)相互作用研究提供可靠工具，滿足基礎(chǔ)研究中對(duì)GRB2蛋白精準(zhǔn)檢測(cè)的需求。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2), GRB2, ASH
  • 反應(yīng)種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide derived from human GRB2
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    6E1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.; Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • 基因功能參考文獻(xiàn)：
    1. that miR-1258 can suppress NSCLC progression by targeting the GRB2/Ras/Erk pathway PMID: 30069987
    2. The binding site of miR-433-3p was identified in the 3'UTR region of GRB2. Western blotting and FQ-PCR showed that miR-433-3p inhibited the mRNA and protein expression of GRB2. PMID: 29730656
    3. study unravels a unique role of Grb2 in protecting the cytoskeletal architecture in AD-like conditions and presents a potential new strategy for controlling neurodegeneration PMID: 28360125
    4. M. tuberculosis-initiated human mannose receptor signaling regulates macrophage recognition and vesicle trafficking by gamma Fc receptors, Grb2, and SHP-1. PMID: 28978467
    5. Data indicate GRB2 as a direct target of miR-329 in pancreatic cancer cells, and expression of GRB2 was inversely correlated with miR-329 expression in pancreatic cancer patients. PMID: 26885689
    6. EGFR colocalization with GRB2 as assessed by PLA is not correlated with EGFR expression levels or mutation status, defining a patient group that may show EGFR pathway activation, as illustrated by its prognostic value. PMID: 27449805
    7. Low GRB2 expression is associated with Breast Cancer. PMID: 23826330
    8. ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP are potent reference genes in neuroendocrine tumors of the lung. PMID: 27802291
    9. Following phosphorylation of the tyrosine, the proteins growth factor receptor-bound protein 2 (Grb2), Grb2-related adaptor downstream of Shc (Gads), and p85 subunit of phosphoinositide 3-kinase may bind to pYMNM (where pY is phosphotyrosine) via their Src homology 2 (SH2) domains, leading to downstream signaling to distinct immune pathways. These three adaptor proteins bind to the same site on CD28 with variable affinity PMID: 27927989
    10. investigated the target and mechanism of miR-411-5p in breast cancer using mimic and inhibitor, and demonstrated the involvement of GRB2 and Ras activation PMID: 27264952
    11. The study presents EPR spectroscopic analysis of the binding of the wild-type and mutant Grb2 SH2 domains to the CSpYVNVQC peptide variant. These data confirm that the binding specificity of SH2 domains, even that of Grb2, results from multiple factors and does not depend merely on the nature of one particular residue. PMID: 26645482
    12. TGF-beta2 induces Grb2 to recruit PI3-K to TGF-RII that activates JNK/AP-1-signaling and augments invasiveness of Theileria-transformed macrophages. PMID: 26511382
    13. Genetic variation near GRB2 on chromosome 17q25.1 is associated with sight-threatening diabetic retinopathy. PMID: 26188370
    14. Only monomeric Grb2 is capable of upregulating MAP kinase signalling. The dimeric state is inhibitory to this process. The self-association/dissociation of Grb2 represents a switch that regulates MAP kinase activity and hence controls cancer progression. PMID: 26103942
    15. The immunoglobulin tail tyrosine motif in the cytoplasmic segments of membrane-bound IgGs acts as the principle signal amplifier by incorporating a Grb2-Btk signaling. PMID: 25413232
    16. findings show that regions outside of the consensus PxxPxR sites drive the high affinity association of GRB2 with SH3 domain ligands PMID: 26079855
    17. down-regulated molecule growth factor receptor-bound protein 2 (GRB2) was a prominent node in fourteen cell proliferation-related sub-pathways. PMID: 25659534
    18. GRB2 was an independent factor in prognosis of esophageal squamous cell carcinoma patients PMID: 25031732
    19. In addition, GRB2 and UBC were found to be hub nodes in the differentially expressed subnetwork and may be used as potential biomarkers or targets for the diagnosis of bladder cancer. PMID: 25688510
    20. The N-terminal SH3 domain of Grb2 specifically interacts with UVRAG, unlike the C-terminal SH3 domain. This interaction helps to understand the role of Grb2 in the autophagic maturation of vesicles. PMID: 24882360
    21. Knockdown of GRB2 suppressed LMTK3-induced CDC42 activation, blocked ITGA5 and ITGB1 expression promoted by the transcription factor serum response factor (SRF), and reduced invasive activity PMID: 24939894
    22. these results identify Grb2 and Shc as central signaling effectors of Met-driven progression of intestinal epithelial-derived cancers. Notably, they suggest that Grb2 may represent a promising target for the design of novel colorectal cancer therapies. PMID: 24708867
    23. SGEF interacts with Grb2. PMID: 24399467
    24. Trafficking of EGFR and Grb2 in living HeLa cells stimulated with low, physiological concentrations of EGFR ligands, was analyzed.. PMID: 24259669
    25. In neuronal cells, amyloid-beta protein precursor intracellular domain co-localizes with GRB2 in cell compartments. PMID: 24100123
    26. Recruitment of the adaptor protein Grb2 to EGFR tetramers. PMID: 24697349
    27. suppression of Grb2 expression in HepG2 cells improved hepatic steatosis, glucose metabolism, oxidative stress, and apoptosis induced by palmitic acid incubation partly though modulating the insulin signaling pathway. PMID: 23771793
    28. Epigenetic repression of miR-376c accelerated EGF-dependent cell migration through its target GRB2 in HuCCT1 cells. PMID: 23922722
    29. a central role for adaptor proteins p66Shc and Grb2 in the regulation of ARF1 and ARF6 activation in invasive breast cancer cells. PMID: 24407288
    30. A deficient lipid rafts recruitment of CD3zeta/ZAP-70/Grb2, and these proteins do not merge with GM1 within the lipid rafts. PMID: 23916875
    31. EGFR interacts with Grb2 in both constitutive and EGF-dependent manners, whereas the HER3-Grb2 interaction requires the heteromerization of EGFR and HER3. PMID: 23700486
    32. VP11/12 SFK-binding motifs recruit Lck and the activated Src family kinase then leads (directly or indirectly) to phosphorylation of additional motifs involved in recruiting p85, Grb2, and Shc. PMID: 23946459
    33. the N-terminal SH3-SH2 region of grb2 protein was in charge of binding with phosphorylated TIGIT. PMID: 23154388
    34. GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. PMID: 23460737
    35. Grb2 is thereby recruited into the plexin-B1 receptor complex and, through its SH3 domain, interacts with p190 RhoGAP and mediates RhoA deactivation. PMID: 22505611
    36. Grb2 exerts constitutive control over the mutually dependent activities of FGFR2 and Shp2. PMID: 23420874
    37. The discovery of a negative regulatory role for Grb2 reveals that this adaptor acts as a double-edged sword in the regulation of RTK signaling. PMID: 23131845
    38. The Dok-3/Grb2 protein signal module attenuates Lyn kinase-dependent activation of Syk kinase in B cell antigen receptor microclusters PMID: 23223229
    39. The results fit with a role of Grb2 in protein aggregation, achieving specificity by multivalent interactions, despite the relatively low affinity of single SH3 interactions. PMID: 23159538
    40. Grb2 interacts with ADAM12 and is required for ADAM12 internalization. PMID: 22882974
    41. intracellular Francisella tularensis novicida triggers temporal and early activation of Ras through the SOS2/GrB2/PKCalpha/PKCbetaI quaternary complex; Ras signalling by intracellular F. tularensis is essential for intracellular proliferation in the cytosol PMID: 20618341
    42. the negative loop on p38 is mediated by c-ABL phosphorylation at tyrosine 105 of the adaptor protein NCK1, while the phosphorylation at tyrosine 209 of GRB2 down-modulates ERK1/2 and JNKs signaling. PMID: 22327338
    43. T-cell antigen receptor (TCR)-mediated Erk activation requires RasGRP1, but not Grb2/Sos. PMID: 22344067
    44. Modeling and simulation of aggregation of membrane protein LAT with molecular variability in the number of binding sites for cytosolic Grb2-SOS1-Grb2 PMID: 22396725
    45. multiple binding sites within Sos1 provide a physical route for Grb2 to hop in a flip-flop manner from one site to the next through facilitated diffusion, and such rapid exchange forms the basis of cooperativity driving bivalent binding of Grb2 to Sos1 PMID: 22360309
    46. Hypoxia can induce the activation of cytoplasmic FAK, Grb2 and paxillin so as to regulate the migration, survival and proliferation of pulmonary artery smooth cells. PMID: 22094095
    47. Our data reliably define context-specific and time-dependent networks that form around GRB2 after stimulation, and reveal core and growth factor-selective complexes comprising 90 proteins identified as interacting with GRB2 in HEK293T cells. PMID: 21706016
    48. ACAP4 protein cooperates with Grb2 protein to orchestrate epidermal growth factor-stimulated integrin beta1 recycling in cell migration PMID: 22027826
    49. High-resolution crystal structures of Grb2-SH2 domain bound to AICD peptides reveal a unique mode of binding where the peptides assume a noncanonical conformation that is unlike other structures of AICD peptides bound to protein-tyrosine-binding domains PMID: 22001015
    50. RhoU physically associates with activated EGFR in a GRB2-dependent manner; suppression of GRB2 by RNA interference abrogates the interaction of RhoU with activated EGFR. PMID: 21508312

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm. Endosome. Golgi apparatus.
  • 蛋白家族：
    GRB2/sem-5/DRK family
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 4566

    OMIM: 108355

    KEGG: hsa:2885

    STRING: 9606.ENSP00000339007

    UniGene: Hs.444356