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  4. HSP90AA1/HSP90AB1 Recombinant Monoclonal Antibody

HSP90AA1/HSP90AB1 Recombinant Monoclonal Antibody

  • 中文名稱：
    HSP90AA1/HSP90AB1重組抗體
  • 貨號：
    CSB-RA010802A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
      All lanes: Hsp90 alpha + beta antibody at 1.25μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 90 KDa
      Observed band size: 90 KDa
    • Immunoprecipitating Hsp90 alpha + beta in Hela whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-RA010802A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-RA010802A0HU (3μg) + Hela whole cell lysate (500μg)
      Lane 3: Hela whole cell lysate (20μg)
    • Overlay histogram showing Jurkat cells stained with CSB-RA010802A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA010802A0HU重組單克隆抗體是針對HSP90AA1/HSP90AB1靶點開發(fā)的高特異性試劑，適用于多種實驗場景。HSP90AA1和HSP90AB1是熱休克蛋白90家族成員，作為關(guān)鍵分子伴侶參與蛋白折疊、細胞應(yīng)激響應(yīng)及信號轉(zhuǎn)導(dǎo)調(diào)控，其功能異常與腫瘤發(fā)生發(fā)展、神經(jīng)退行性病變及炎癥反應(yīng)等病理過程密切相關(guān)。本抗體經(jīng)嚴格驗證，可在ELISA、Western Blot（WB）、流式細胞術(shù)（FC）和免疫沉淀（IP）中表現(xiàn)優(yōu)異，推薦使用稀釋度為WB 1:500-1:5000、IP 1:200-1:1000，具有批次間穩(wěn)定性和低交叉反應(yīng)性特點。實驗數(shù)據(jù)顯示，該抗體能特異性識別目標蛋白，在細胞裂解液及多種組織樣本中均展現(xiàn)出清晰的檢測信號，適用于探究HSP90在蛋白質(zhì)穩(wěn)態(tài)調(diào)控、腫瘤微環(huán)境調(diào)節(jié)或藥物靶點篩選等領(lǐng)域的分子機制。其應(yīng)用場景涵蓋基礎(chǔ)研究中的蛋白定位分析、相互作用組學研究以及疾病相關(guān)信號通路的功能驗證，為科研人員提供可靠的實驗工具。本產(chǎn)品僅限科學研究使用。
  • Uniprot No.：
  • 反應(yīng)種屬：
    Human, Rat
  • 免疫原：
    A synthesized peptide derived from human HSP90AA1/HSP90AB1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    10D6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC, IP
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IP 1:200-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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