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  4. JUNB Recombinant Monoclonal Antibody

JUNB Recombinant Monoclonal Antibody

  • 中文名稱：
    JUNB重組抗體
  • 貨號：
    CSB-RA988421A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA988421A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of MCF7 Cells with CSB-RA988421A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA988421A0HU JUNB重組單克隆抗體是針對轉(zhuǎn)錄因子JUNB開發(fā)的高特異性科研工具。該抗體靶向的JUNB蛋白屬于激活蛋白1（AP-1）家族成員，在細胞增殖、分化調(diào)控及應(yīng)激響應(yīng)等生物學(xué)過程中發(fā)揮重要作用，其異常表達與腫瘤發(fā)生和免疫調(diào)控密切相關(guān)。經(jīng)多平臺驗證，本抗體在酶聯(lián)免疫吸附試驗（ELISA）中展現(xiàn)優(yōu)異的線性檢測范圍，在免疫組織化學(xué)（IHC）和免疫熒光（IF）實驗中可清晰定位JUNB蛋白在細胞核內(nèi)的表達特征，推薦使用稀釋度分別為IHC 1:50-1:200和IF 1:20-1:200。實驗數(shù)據(jù)表明，該抗體在人源組織切片及培養(yǎng)細胞樣本中均呈現(xiàn)高度一致性，能有效識別天然構(gòu)象與變性處理的JUNB蛋白。適用于探索JUNB在腫瘤微環(huán)境中的作用機制、炎癥信號通路調(diào)控研究以及發(fā)育生物學(xué)中細胞命運決定等科研領(lǐng)域，特別適合通過多重染色技術(shù)進行蛋白共定位分析。本產(chǎn)品通過重組表達技術(shù)生產(chǎn)，具有批次間穩(wěn)定性高的優(yōu)勢，適用于基礎(chǔ)科研中蛋白表達譜分析及功能研究。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Transcription factor jun-B, JUNB
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human JunB
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    5H4
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
  • 基因功能參考文獻：
    1. It has been shown that the AP-1 family member JunB and retinoic acid receptor alpha (RARa) mediate catalase transcriptional activation and repression, respectively, by controlling chromatin remodeling through a histone deacetylases-dependent mechanism. PMID: 27591797
    2. JunB neddylation mediated by Itch promotes its ubiquitination-dependent degradation. PMID: 27245101
    3. a specific role for AP-1/JunB in multiple myeloma cell proliferation, survival and drug resistance. PMID: 27890927
    4. VEGF-induced endothelial migration is mediated primarily by induction of JunB whereas the promotion of endothelial proliferation by VEGF is mediated by JunB-independent AP-1 family members. PMID: 26860974
    5. Results suggested that JunB could play an important role in promoting cell invasion, migration and distant metastasis in head and neck squamous cell carcinoma via pathways other than epithelial-to-mesenchymal transition. PMID: 26754630
    6. Highly recurrent mutation of JUNB is associated with nodular lymphocyte predominant Hodgkin lymphoma. PMID: 26658840
    7. ETS2, HNF4A and JUNB are synergistic master regulators of epithelial-to-mesenchymal transition in cancer. PMID: 26926107
    8. PDK1 functions as a tumor promoter in human gallbladder cancer by upregulating JunB, promoting epithelial mesenchymal transformation, and cell migration. PMID: 26318166
    9. Our findings demonstrate that miRNA-149* may serve as an oncogenic regulator in T-cell acute lymphoblastic leukemia by negatively regulating JunB PMID: 26725775
    10. The MAPK pathway plays a primary role in the control of JUNB gene expression. PMID: 25662951
    11. JunB is likely to be a key target of c-Abl in expression of p21 in Adriamycin-induced DDR. PMID: 26217035
    12. Caveolin 2 disengages repressed Egr-1 and JunB promoters from lamin A/C through disassembly of H3K9me3 in the inner nuclear membrane. PMID: 25753664
    13. JunB expression was significantly increased while cyclin-D1 expression was significantly down-regulated in pre-eclampsia relative to control placental mesenchymal stromal cells. PMID: 24780198
    14. findings uncover the oncogenic role of the JUNB/CD30 axis and its potential as therapeutic target in ALK+ ALCL. PMID: 25145835
    15. These results demonstrate a unique induction of JUNB in response to kinase inhibitor therapies that may be among the earliest events in the progression to treatment resistance. PMID: 24858691
    16. Apoptosis rate of HepG2 cells transformed with pEGFP-C1-wtp53/JunB was significantly higher. PMID: 23259178
    17. Data demonstrate that S100A14 is transcriptionally regulated by JunB and involved in esophageal squamous cell carcinoma cell differentiation. PMID: 24107296
    18. an important role of the A2B receptor-dependent upregulation of JunB in VEGF production and possibly other AP-1-regulated events. PMID: 24136993
    19. JUNB was specifically expressed in human immune cells during acute liver injury. PMID: 24200694
    20. JunB is shown to be regulated at a post-transcriptional level during EC activation. In activated EC, the AP-1 transcription factor JunB, is regulated on a post-transcriptional level. PMID: 23297064
    21. JunB regulates visceral smooth muscle cell contractility through effects on both myosin and the actin cytoskeleton. PMID: 23308222
    22. A novel mechanism by which mitosis progression and chromatid cohesion are regulated through GSK3/SCF(FBXW7)-mediated proteolysis of JunB. PMID: 22710716
    23. Study confirmed that JunB was upregulated in VHL-defective clear-cell renal-cell carcinoma (ccRCC) specimens by immunostaining. Short-hairpin RNA (shRNA)-mediated knockdown of JunB in 786-O and A498 VHL null ccRCC cells suppressed their invasiveness. PMID: 22020339
    24. Different mechanisms preserve translation of programmed cell death 8 and JunB in poliovirus-infected endothelial cells. PMID: 22328780
    25. up-regulation of JunB induced by HGF might play an important role in the regulation of cell proliferation and cell invasion through MMP-9 expression. PMID: 22252121
    26. JunB activates aromatase promoters by maintaining JunD expression PMID: 21393445
    27. The methylation of JunB and CDH13 gene promoters probably plays a role in the pathogenesis of chronic myelogenous leukemia and may have clinical significance in predicting prognosis of CML. PMID: 20030915
    28. JunB is a direct transcriptional activator of GzB and that GzB transcription is also promoted by NPM-ALK. PMID: 21326808
    29. Monoammonium glycyrrhizinate highly stimulated JUNB expression in a human hepatoma cell line, HepG2. PMID: 21225234
    30. The increase in JunB expression attenuated nuclear relocation of apoptosis-inducing factor and mitochondrial Bcl-2 reduction that occurred following hydrogen peroxide exposure. PMID: 20132737
    31. JunB functions as a transcriptional factor and up-regulates the expression of VEGF. PMID: 20056077
    32. This analysis shows that the overwhelming majority of JUNB alleles in both chronic phase and blast crisis samples remain unmethylated PMID: 20006998
    33. When p53 dysfunction and low expression of JunB are simultaneous, they may play an important role in down-regulating the expression of KAI1 by synergism in hepatocellular carcinoma. PMID: 19666408
    34. JunB is an important regulator of erythroid differentiation PMID: 11726656
    35. JunB potentiates function of BRCA1 activation domain 1 (AD1) through a coiled-coil-mediated interaction PMID: 12080089
    36. demonstrated that a functional AP-1 site mediates MMP-2 transcription in cardiac cells through the binding of distinctive Fra1-JunB and FosB-JunB heterodimers. The synthesis of MMP-2 is considered to be independent of the AP-1 transcriptional complex PMID: 12371906
    37. results have revealed, for the first time, amplification and expression patterns of JUNB in primary cutaneous lymphomas PMID: 12393503
    38. Real-time RT-PCR gave further insights into the role of JunB in human CML. The expression levels were significantly impaired in CML cases. In the promoter area, most of the CpG sites were methylated only in CML cases. PMID: 12506033
    39. C/EBPalpha and PKC/delta affect expression of this gene and monocyte differentiation. PMID: 12522006
    40. Expression of junB was induced by TPA and Saikosaponin a during 30 min to 6 h of treatment. PMID: 12592382
    41. JunB was strongly expressed in T-cell lymphomas, but non-Hodgkin B-cell lymphomas do not or only weakly express JunB. PMID: 12907453
    42. Transcription factor c-Jun plays a principal role in down-regulation of mdr-1 expression and induction of apoptosis in salvicine-treated human MDR K562/A02 cells. PMID: 12907627
    43. The IGFBP3, hRas, JunB, Egr-1, Id1 and MIDA1 genes were up-regulated in psoriatic involved skin compared with uninvolved skin. PMID: 16552541
    44. 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole sensitivity-inducing factor (DSIF)- and NELF-mediated transcriptional pausing has a dual function in regulating immediate-early expression of the human junB gene. PMID: 16880520
    45. JunB and JunD contribute opposing effects; JunB activated whereas JunD repressed heme oxygenase-1 expression in human renal epithelial cells PMID: 17204476
    46. results suggest that HTLV-I HBZ-SP1- mediated sequestration of JunB to the HBZ-SP1 nuclear bodies may be causing the repression of JunB activity in vivo PMID: 17306025
    47. JunB is a critical target of mTOR and is translationally regulated in NPM-ALK-positive lymphomas. PMID: 17690253
    48. constitutive action of aberrantly expressed JunB on hypomethylated CD30 CpG islands of lymphocytes triggers CD30 induction and initiates activation of the JunB-CD30-JunB loop, essential to the pathogenesis of HL and ALCL PMID: 17965727
    49. JunB levels, which are high in S phase, drop during mid- to late G2 phase due to accelerated phosphorylation-dependent degradation by the proteasome, and are required for subsequent reduction of cyclin A2 levels in prometaphase PMID: 18391017
    50. sumoylation of JunB regulates its ability to induce cytokine gene transcription and likely plays a critical role in T cell activation. PMID: 18424718

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    BZIP family, Jun subfamily
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6205

    OMIM: 165161

    KEGG: hsa:3726

    STRING: 9606.ENSP00000303315

    UniGene: Hs.25292