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METAP2 Recombinant Monoclonal Antibody

  • 中文名稱：
    METAP2重組抗體
  • 貨號(hào)：
    CSB-RA294332A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA294332A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    METAP2 Recombinant Monoclonal Antibody（CSB-RA294332A0HU）是針對(duì)甲硫氨酸氨基肽酶2（METAP2）開(kāi)發(fā)的高特異性抗體，適用于酶聯(lián)免疫吸附試驗(yàn)（ELISA）和免疫組織化學(xué)（IHC）檢測(cè)。METAP2是一種高度保守的金屬酶，在真核生物中負(fù)責(zé)催化新生蛋白質(zhì)N端甲硫氨酸的切除，參與調(diào)控蛋白質(zhì)成熟過(guò)程，其異常表達(dá)與細(xì)胞增殖、血管生成等病理過(guò)程密切相關(guān)。該抗體經(jīng)嚴(yán)謹(jǐn)驗(yàn)證，在IHC實(shí)驗(yàn)中可清晰呈現(xiàn)METAP2蛋白在福爾馬林固定石蠟包埋組織中的定位表達(dá)，推薦使用1:50至1:200稀釋度進(jìn)行染色優(yōu)化，實(shí)驗(yàn)結(jié)果展現(xiàn)出良好的信號(hào)強(qiáng)度和低背景干擾。同時(shí)，其ELISA檢測(cè)性能通過(guò)抗原結(jié)合特異性驗(yàn)證，適用于定量分析樣本中METAP2的表達(dá)水平。該產(chǎn)品可作為研究METAP2在腫瘤微環(huán)境調(diào)控、代謝性疾病相關(guān)蛋白加工機(jī)制中的有效工具，廣泛應(yīng)用于細(xì)胞生物學(xué)、疾病分子機(jī)制等基礎(chǔ)研究領(lǐng)域，為蛋白質(zhì)功能研究及病理狀態(tài)分析提供可靠支持。重組單克隆抗體技術(shù)保障了產(chǎn)品的高批次間一致性。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Methionine aminopeptidase 2 (MAP 2) (MetAP 2) (EC 3.4.11.18) (Initiation factor 2-associated 67 kDa glycoprotein) (p67) (p67eIF2) (Peptidase M), METAP2, MNPEP P67EIF2
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human Methionine Aminopeptidase 2
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    8F2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.; Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
  • 基因功能參考文獻(xiàn)：
    1. MetAP1 and MetAP2 have roles in driving cell selectivity for a potent anti-cancer agent in synergy, by controlling glutathione redox state PMID: 27542228
    2. Suggest MetAP2 as important regulator of proliferation/apoptosis in non-small cell lung cancers. PMID: 26935506
    3. Nt-acetylation prevents the excision of the initiator methionine by MetAP2. PMID: 25886145
    4. Data indicate that methionine aminopeptidase 2 (MetAP2) contains a single disulfide bond that exists in oxidized and reduced states and influences enzyme function. PMID: 24700462
    5. The activity of unmodified, nitrated and oxidised METAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in METAP2. PMID: 24041691
    6. Increased expression of METAP2 is associated with pilocytic astrocytoma. PMID: 23161775
    7. This study demonstrated a hitherto-undescribed role of MetAP2 in definitive hematopoiesis and a possible link to noncanonical Wnt and ERK signaling. PMID: 21937698
    8. the substrate specificities of Escherichia coli MetAP1, human MetAP1, and human MetAP2 were systematically profiled PMID: 20521764
    9. Data indicate that MetAP2 expression and pp60c-src phosphorylation were decreased in NC2213 treated cells. PMID: 19703310
    10. the functional role of S100A4 in regulating endothelial cell growth and tumor metastasis involves interaction with the N-terminal half of Methionine Aminopeptidase 2. PMID: 11994292
    11. High expression in germinal center B cells and their neoplastic counterparts. PMID: 12118091
    12. Divalent metal cofactors are physiologically relevant for activity of this enzyme. PMID: 12718546
    13. MetAP protein has a role in colorectal adenocarcinoma progression PMID: 15102683
    14. Expressed in colon cancer cells PMID: 15336565
    15. MetAP-1 and MetAP-2 have essential functions in the control of mammalian cell proliferation PMID: 15962312
    16. A comparison of the structual differences between Type I and Type II methionine aminopeptidases. PMID: 16274222
    17. Data suggest that methionine aminopeptidase 2 plays a role in the proliferation of fibroblasts and myofibroblasts in fibrotic lung diseases and may serve as a novel pharmacologic target in idiopathic pulmonary fibrosis. PMID: 17446530
    18. MetAP2 plays an important role in tumor cell growth and may contribute to tumorigenesis PMID: 18264137
    19. Discovery, identification, and characterization of candidate pharmacodynamic markers of METAP2 inhibition are reported. PMID: 18828628
    20. Using bovine cell extract, fumagillin was found to be bound by methionine aminopeptidase (MetAP-2). PMID: 9177176

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  • 亞細(xì)胞定位：
    Cytoplasm. Note=About 30% of expressed METAP2 associates with polysomes.
  • 蛋白家族：
    Peptidase M24A family, Methionine aminopeptidase eukaryotic type 2 subfamily
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 16672

    OMIM: 601870

    KEGG: hsa:10988

    STRING: 9606.ENSP00000325312

    UniGene: Hs.444986