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  4. MGEA5 Recombinant Monoclonal Antibody

MGEA5 Recombinant Monoclonal Antibody

  • 中文名稱：
    MGEA5重組抗體
  • 貨號：
    CSB-RA833565A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate
      All lanes: MGEA5 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 103, 96, 77, 97 kDa
      Observed band size: 130 kDa
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA833565A0HU MGEA5重組單克隆抗體是經(jīng)ELISA和Western Blot嚴格驗證的高特異性科研工具。該抗體針對腦膜瘤表達抗原5（MGEA5）靶點設計，該蛋白作為O-GlcNAcase酶參與細胞信號轉(zhuǎn)導和表觀遺傳調(diào)控，在糖代謝異常相關(guān)研究中具有重要價值。實驗數(shù)據(jù)顯示其在Western Blot中能特異性識別天然及重組MGEA5蛋白，推薦使用稀釋比例為1:500至1:5000，適用于檢測多種樣本中的蛋白表達水平。產(chǎn)品適用于體外實驗環(huán)境下的蛋白功能研究、疾病機制探索及分子互作分析，可為代謝性疾病、腫瘤生物學等領域的科研工作提供可靠支持。重組單克隆抗體技術(shù)確保批次間高度一致性，配套優(yōu)化的實驗方案助力研究人員快速獲得可重復的實驗結(jié)果。
  • Uniprot No.：
  • 基因名：
    MGEA5
  • 別名：
    Protein O-GlcNAcase (OGA) (EC 3.2.1.169) (Beta-N-acetylglucosaminidase) (Beta-N-acetylhexosaminidase) (Beta-hexosaminidase) (Meningioma-expressed antigen 5) (N-acetyl-beta-D-glucosaminidase) (N-acetyl-beta-glucosaminidase) (Nuclear cytoplasmic O-GlcNAcase and acetyltransferase) (NCOAT), MGEA5, HEXC KIAA0679 MEA5
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human MGEA5
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    8D12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.; Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
  • 基因功能參考文獻：
    1. Beta-N-acetylhexosaminidase substrate recognition and specificity PMID: 28939839
    2. Tax interacts with the host OGT/OGA complex and inhibits the activity of OGT-bound OGA. PMID: 28742148
    3. TGFBR3 and/or MGEA5 rearrangements are much more common in hybrid hemosiderotic fibrolipomatous tumor-myxoinflammatory fibroblastic sarcomas than in classical myxoinflammatory fibroblastic sarcomas. PMID: 26980036
    4. Data suggest that the substrate specificity of O-GlcNAcase/OGA does not extend to proteins/peptides modified with S-GlcNAc (an analog of O-GlcNAc); proteins modified with S-GlcNAc appear to be stable against O-GlcNAcase/OGA hydrolysis. PMID: 28627871
    5. hOGA forms an unusual arm-in-arm homodimer in which the catalytic domain of one monomer is covered by the stalk domain of the sister monomer to create a substrate-binding cleft. PMID: 28319083
    6. the O-linked N-acetylglucosamine (O-GlcNAc) processing enzymes, O-GlcNAc-transferase (OGT) and O-GlcNAcase (OGA), interact with the (A)gamma-globin promoter at the -566 GATA repressor site PMID: 27231347
    7. E2F1 negatively regulates both Ogt and Mgea5 expression in an Rb1 protein-dependent manner. PMID: 26527687
    8. OGA overexpression in endothelial cells improves endothelial function and may have a beneficial effect on coronary vascular complications in diabetes. PMID: 26269457
    9. Amino acid composition of splice variants, post-translational modifications, and stable associations with regulatory proteins influence subcellular distribution/substrate specificity of OGA and OGT (O-linked N-acetylglucosamine transferase). [REVIEW] PMID: 25173736
    10. This work identifies the first target of miR-539 in the heart and the first miRNA that regulates OGA. PMID: 25183011
    11. Report the presence of TGFBR3 and/or MGEA5 rearrangements in pleomorphic hyalinizing angiectatic tumors and the spectrum of related neoplasms. PMID: 24705316
    12. Estrogen replacement therapy and plyometric training influence muscle OGT and OGA gene expression, which may be one of the mechanisms by which HRT and PT prevent aging-related loss of muscle mass. PMID: 24365779
    13. O-linked beta-N-acetylglucosaminylation (O-GlcNAcylation) in primary and metastatic colorectal cancer clones and effect of N-acetyl-beta-D-glucosaminidase silencing on cell phenotype and transcriptome. PMID: 22730328
    14. Data show that the interplay between O-GlcNAc and phosphorylation on proteins and indicate that these effects can be mediated by changes in hOGT and hOGA kinetic activity. PMID: 22311971
    15. Analysis of urinary content of MGEA5 and OGT may be useful for bladder cancer diagnostics. PMID: 22783592
    16. Decrease in MGEA5 and increase in O-GlcNAc transferease expression in higher grade tumors suggests that increased O-GlcNAc modification may be implicated in breast tumor progression and metastasis. PMID: 21567137
    17. Chromosomal translocation t(1;10) is consistent with rearrangements of TGFBR3 and MGEA5 in both myxoinflammatory fibroblastic sarcoma and hemosiderotic fibrolipomatous tumor. PMID: 21717526
    18. Reducing ChREBP(OG) levels via OGA overexpression decreased lipogenic protein content (ACC, FAS), prevented hepatic steatosis, and improved the lipidic profile of OGA-treated db/db mice. PMID: 21471514
    19. Direct evidence links muscle atrophy and the disruption of O-GlcNAcase activity in male bitransgenic mice. PMID: 21178104
    20. Results provide evidence that OGA may possess a substrate-recognition mechanism tinvolving interactions with O-GlcNAcylated proteins beyond the GlcNAc-binding site. PMID: 20863279
    21. N-terminal region of OGA contains the catalytic site and the C-terminal region stabilizes the protein structure and affects substrate affinity. PMID: 20673219
    22. This study analyzes the activity of the enzyme involved in the removal of these sugar residues, i.e. beta-N-acetylglucosaminidase (O-GlcNAcase) as well as the level of N-acetylglucosamine in benign and malignant thyroid lesions. PMID: 20198314
    23. Investigated this locus in Pima Indians who have the world's highest prevalence of NIDDM. Concluded that mutations in MGEA5 are unlikely to contribute to NIDDM in this population PMID: 12359146
    24. In type 2 Diabetes patients in Mexico City, the frequency of the T allele of MGEAT5 was higher (2.6%) in the cases than in controls (1.8%), but not a significant deviation from Hardy_Weinberg proportions. PMID: 17546623
    25. review of modifications, phosphorylation and a specific form of glycosylation, O-linked -N-acetylglucosaminylation by O-GlcNAc, relevant to pathological tau phosphorylation PMID: 18641620
    26. the short nuclear variant of O-GlcNAcase, which has the identical catalytic domain as the full-length enzyme, has similar trends in a pH-rate profile and Taft linear free energy analysis as the full-length enzyme PMID: 19423084
    27. characterization of O-GlcNAcase transition states using several series of substrates to generate multiple simultaneous free-energy relationships PMID: 19715310
    28. This protein is a cytosolic, neutral, O-GlcNAc specific hexosaminidase termed O-GlcNAcase. PMID: 11148210

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  • 亞細胞定位：
    [Isoform 3]: Nucleus.; [Isoform 1]: Cytoplasm.
  • 蛋白家族：
    Glycosyl hydrolase 84 family
  • 組織特異性：
    Ubiquitous. Shows highest expression in the brain, placenta and pancreas.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 7056

    OMIM: 604039

    KEGG: hsa:10724

    STRING: 9606.ENSP00000354850

    UniGene: Hs.500842