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POU2AF1 Recombinant Monoclonal Antibody

  • 中文名稱：
    POU2AF1重組抗體
  • 貨號(hào)：
    CSB-RA280291A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Raji whole cell lysate
      All lanes: BOB1 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 28 kDa
      Observed band size: 35 kDa
    • IHC image of CSB-RA280291A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA280291A0HU diluted at 1:100 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    POU2AF1重組單克隆抗體（CUSABIO貨號(hào)：CSB-RA280291A0HU）是針對(duì)B細(xì)胞特異性轉(zhuǎn)錄共激活因子POU2AF1開發(fā)的高特異性科研試劑。POU2AF1在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用，通過結(jié)合POU結(jié)構(gòu)域轉(zhuǎn)錄因子調(diào)控B細(xì)胞發(fā)育及抗體生成相關(guān)基因的表達(dá)，其異常表達(dá)與淋巴系統(tǒng)疾病密切相關(guān)。本抗體經(jīng)ELISA、Western Blot（WB）和免疫組織化學(xué)（IHC）多平臺(tái)驗(yàn)證，在WB實(shí)驗(yàn)中于1:500至1:5000稀釋范圍內(nèi)可清晰檢測靶蛋白，IHC應(yīng)用在1:50至1:200稀釋比例下能精準(zhǔn)定位福爾馬林固定石蠟包埋組織中的抗原。實(shí)驗(yàn)數(shù)據(jù)證實(shí)該抗體具有優(yōu)異的選擇性，可識(shí)別天然構(gòu)象和重組表達(dá)的POU2AF1蛋白，且批間一致性穩(wěn)定。適用于探索B細(xì)胞分化機(jī)制、免疫調(diào)控網(wǎng)絡(luò)及淋巴瘤相關(guān)分子病理研究，為免疫學(xué)、血液腫瘤學(xué)等領(lǐng)域的基礎(chǔ)研究提供高效檢測工具，特別適用于體外模型構(gòu)建、蛋白質(zhì)互作分析及組織表達(dá)譜研究等實(shí)驗(yàn)場景。本產(chǎn)品專為科研實(shí)驗(yàn)設(shè)計(jì)，不涉及其他用途。
  • Uniprot No.：
  • 基因名：
    POU2AF1
  • 別名：
    POU domain class 2-associating factor 1 (B-cell-specific coactivator OBF-1) (BOB-1) (OCA-B) (OCT-binding factor 1), POU2AF1, OBF1
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human BOB1
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    4A5
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator.
  • 基因功能參考文獻(xiàn)：
    1. The number of follicular B2 lymphocytes and expression of the B-cell-specific transcriptional coactivator OcaB increase with age in spleen and in intra-abdominal epididymal white adipose tissue (eWAT), concomitantly with higher circulating levels of IgG and impaired glucose homeostasis. PMID: 29496744
    2. These findings demonstrate for the first time that functional polymorphism in the 3'-UTR of POU2AF1 is associated with susceptibility, and that single-nucleotide polymorphisms interaction with hsa-miR-633 affects gene expression and increases the risk of lymphoma. PMID: 28345816
    3. Oct2 and Bob1 are very reliable in determining B cell lineage in the absence of expression of other pan-B cell markers PMID: 27319306
    4. these findings suggest a novel function of POU2AF1 as a potential regulator of host defense genes in the human airway epithelium. PMID: 26927796
    5. It has been identified as a new disease susceptibility gene among Japanese. Though different from Europeans, it is indicated that a B lymphocyte differentiation route shares a common disease developing process. [Review] PMID: 24005100
    6. Two significant susceptibility loci, TNFSF15 (rs4979462) and POU2AF1 (rs4938534) (combined odds ratio [OR] = 1.56, p = 2.84 x 10(-14) for rs4979462. PMID: 23000144
    7. genetic polymorphism is associated with common variable immunodeficiency PMID: 21905497
    8. Data show that Igh 3' enhancer-bound OCA-B and promoter-bound TFII-I mediate promoter-enhancer interactions, in both cis and trans, that are important for Igh transcription. PMID: 21549311
    9. On multivariate analysis, co-expression of OCT-2/BOB.1 remained predictive for achievement of complete remission and increased risk of relapse. PMID: 20141429
    10. BOB.1 may be helpful marker in the differential diagnosis of classical Hodgkin's lymphoma and primary mediastinal B-cell lymphoma PMID: 20102401
    11. The expression of the octamer cofactor gene OBF-1 (Bob1/OCA-B) is sufficient to override the silencing effects of the B29 silencer, indicating that OBF-1 plays a critical role in B cell-specific B29 promoter expression. PMID: 11907094
    12. enhances transcriptional potential of Oct1 PMID: 12727885
    13. OCA-B sustains expression of the immunoglobulin-secreting program when T lymphoma and plasmacytoma lines are fused, requiring Oct-2 coregulator for its function. PMID: 14662861
    14. POU2AF1 was observed to be differentially expressed in the cells of patients with chronic lymphocytic leukemia. PMID: 15672409
    15. Alteration of the BOB1 locus does not correlate with its suppressed expression in Hodgkin lymphoma. PMID: 15796964
    16. Novel germ cell markers BOB1 were significantly upregulated in seminoma specimens, compared to normal testes. PMID: 17785371
    17. Oct-2 and its cofactor Bob-1 have an important function in mediating the IgH enhancer-bcl-2 promoter region interactions PMID: 18695675

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  • 相關(guān)疾病：
    A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.
  • 亞細(xì)胞定位：
    Nucleus.
  • 蛋白家族：
    POU2AF1 family
  • 組織特異性：
    B-cell specific. Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9211

    OMIM: 601206

    KEGG: hsa:5450

    STRING: 9606.ENSP00000376786

    UniGene: Hs.654525