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Phospho-PAK1/PAK2/PAK3 (S144+S141+S139) Recombinant Monoclonal Antibody

  • 中文名稱：
    磷酸化-PAK1/PAK2/PAK3 (S144+S141+S139)重組抗體
  • 貨號：
    CSB-RA017407A144phHU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in A549 whole cell lysate(treated with EGF or not)
      All lanes Phospho-PAK1/PAK2/PAK3 antibody at 1.2μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 65 KDa
      Observed band size: 65 KDa
    • IHC image of CSB-RA017407A144phHU diluted at 1:100 and staining in paraffin-embedded rat brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA017407A144phHU重組單克隆抗體可特異性識別PAK1/PAK2/PAK3蛋白在S144、S141、S139位點的磷酸化修飾，該位點位于PAK激酶的自抑制結(jié)構(gòu)域，其磷酸化狀態(tài)與PAK家族蛋白的激活及下游信號傳導(dǎo)密切相關(guān)。經(jīng)多平臺驗證，本抗體在Western Blot實驗中可靈敏檢測多種哺乳動物細(xì)胞和組織樣本中內(nèi)源性磷酸化PAK蛋白，推薦稀釋比例為1:500-1:5000；在免疫組化（IHC）實驗中能清晰呈現(xiàn)目標(biāo)蛋白在石蠟包埋組織切片中的亞細(xì)胞定位，推薦稀釋比例為1:50-1:200。該抗體適用于研究PAK信號通路在細(xì)胞運動、腫瘤微環(huán)境調(diào)控以及神經(jīng)突觸可塑性等領(lǐng)域的分子機(jī)制，尤其適用于探究細(xì)胞骨架重構(gòu)、侵襲轉(zhuǎn)移等生物學(xué)過程中PAK激酶的動態(tài)磷酸化調(diào)控。采用重組單克隆技術(shù)制備，具備高批次穩(wěn)定性和特異性，可為細(xì)胞功能研究和疾病相關(guān)蛋白互作分析提供可靠工具。
  • Uniprot No.：
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-PAK1/PAK2/PAK3 (S144+S141+S139)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    3H12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價