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  4. Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody

Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody

  • 中文名稱：
    磷酸化-PAK4/PAK5/PAK6 (S474/S560/S602)重組抗體
  • 貨號(hào)：
    CSB-RA017408A474phHU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in 293 whole cell lysate(treated with Calyculin A or not)
      All lanes Phospho-PAK4/PAK5/PAK6 antibody at 2.15μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 64,75,80 KDa
      Observed band size: 75,80 KDa
    • Immunofluorescence staining of HepG2 cells(treated with 50mM Calyculin A for 30min) with CSB-RA017408A474phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    Phospho-PAK4/PAK5/PAK6 (S474/S560/S602) Recombinant Monoclonal Antibody（CSB-RA017408A474phHU）是一款高特異性抗體，靶向p21激活激酶家族成員PAK4、PAK5和PAK6的磷酸化修飾位點(diǎn)S474、S560及S602。該抗體經(jīng)ELISA、Western blot（WB）和免疫熒光（IF）嚴(yán)格驗(yàn)證，適用于檢測(cè)內(nèi)源性蛋白的磷酸化狀態(tài)。在WB實(shí)驗(yàn)中推薦使用1:500-1:5000的稀釋范圍，可清晰識(shí)別目標(biāo)條帶；IF實(shí)驗(yàn)推薦1:20-1:200稀釋度，能精準(zhǔn)定位蛋白在細(xì)胞內(nèi)的空間分布。PAK4/5/6作為Rho家族GTP酶下游效應(yīng)分子，參與調(diào)控細(xì)胞骨架重組、遷移及生存信號(hào)通路，其磷酸化修飾與激酶活性及功能調(diào)控密切相關(guān)。本抗體可為研究細(xì)胞極性建立、腫瘤侵襲轉(zhuǎn)移、神經(jīng)發(fā)育等領(lǐng)域的分子機(jī)制提供可靠工具，適用于基礎(chǔ)科研中細(xì)胞或組織樣本的蛋白表達(dá)水平分析、亞細(xì)胞定位研究及相關(guān)信號(hào)通路的功能探索。
  • Uniprot No.：
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-PAK4/PAK5/PAK6 (S474/S560/S602)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    2D1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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