搡老女人多毛老妇女中国,日韩亚洲欧美中文高清在线,人妻少妇一区二区三区,色妞色综合久久夜夜,日本熟妇xxxx

Your Good Partner in Biology Research

  1. Home
  2. 抗體
  3. 重組抗體
  4. Phospho-SMAD5 (S463+S465) Recombinant Monoclonal Antibody

Phospho-SMAD5 (S463+S465) Recombinant Monoclonal Antibody

  • 中文名稱：
    磷酸化-SMAD5 (S463+S465)重組抗體
  • 貨號(hào)：
    CSB-RA859108A463phHU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA859108A463phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    Phospho-SMAD5 (S463+S465) Recombinant Monoclonal Antibody（CSB-RA859108A463phHU）是一款特異性識(shí)別SMAD5蛋白雙磷酸化位點(diǎn)（S463和S465）的高靈敏度抗體，適用于研究TGF-β超家族信號(hào)通路的調(diào)控機(jī)制。SMAD5作為TGF-β/BMP信號(hào)轉(zhuǎn)導(dǎo)的關(guān)鍵介質(zhì)，其磷酸化狀態(tài)直接參與調(diào)控細(xì)胞分化、胚胎發(fā)育及組織穩(wěn)態(tài)等生物學(xué)過程。該抗體通過重組單克隆技術(shù)制備，具有高批次穩(wěn)定性和特異性，經(jīng)嚴(yán)格驗(yàn)證可應(yīng)用于ELISA和免疫組化（IHC）實(shí)驗(yàn)，其中IHC推薦使用1:50-1:200稀釋比例，能夠清晰檢測(cè)石蠟包埋組織切片中目標(biāo)蛋白的磷酸化修飾水平。其在信號(hào)通路激活分析、疾病模型研究（如腫瘤微環(huán)境調(diào)控、纖維化疾病或骨骼發(fā)育異常機(jī)制）等科研領(lǐng)域具有重要應(yīng)用價(jià)值，為探索SMAD5依賴的細(xì)胞信號(hào)網(wǎng)絡(luò)及其病理生理功能提供可靠工具。本產(chǎn)品僅限科學(xué)研究使用。
  • Uniprot No.：
  • 基因名：
  • 別名：
    DKFZp781C1895 antibody; DKFZp781O1323 antibody; Dwfc antibody; hSmad5 antibody; JV5 1 antibody; JV5-1 antibody; MAD homolog 5 antibody; MAD, mothers against decapentaplegic homolog 5 antibody; MADH 5 antibody; MADH5 antibody; Mothers against decapentaplegic homolog 5 antibody; mothers against decapentaplegic, drosophila, homolog of, 5 antibody; Mothers against DPP homolog 5 antibody; MusMLP antibody; SMA and MAD related protein 5 antibody; SMAD 5 antibody; SMAD family member 5 antibody; SMAD, mothers against DPP homolog 5 antibody; Smad5 antibody; SMAD5_HUMAN antibody
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-SMAD5 (S463+S465)
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    2G11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
  • 基因功能參考文獻(xiàn)：
    1. We demonstrated high expression of miR-145 associated with late stage and unfavorable prognosis of esophageal cancer. We identified SMAD5 as direct target of miR-145, the suppressed expression of which consequently led to increased cell proliferation and migration/invasion. PMID: 29852786
    2. Smad5 acts as a intracellular pH messenger and maintains the bioenergetic homeostasis of cells by regulating cytoplasmic metabolic machinery. PMID: 28675158
    3. Data suggest that differences in expression levels in granulosa-like tumor cells and granulosa cells (GCs) from patients with polycystic ovary syndrome (PCOS) are due to increased expression of microRNA-27a-3p in GCs caused by insulin resistance in PCOS; microRNA-27a-3 expression is up-regulated in GCs in PCOS; overexpression of miR-27a-3p inhibits SMAD5 expression and promotes apoptosis. (SMAD5 = SMAD family member 5) PMID: 29029022
    4. Here the involvement of the pathway in adult brain function is suggested. This exploratory study establishes a strategy to better identify neuronal molecular signatures that are potentially associated with mental illness and cognitive deficits. We propose that the SMAD pathway may be a novel target in addressing cognitive deficit of SZ in future studies. PMID: 27727244
    5. miR-23a and miR-27a target SMAD5 and regulate apoptosis in human granulosa cells via the FasL-Fas pathway PMID: 26400397
    6. Our findings suggest that suppression of miR-222-3p activity promoted osteogenic differentiation hBMSCs through regulating Smad5-RUNX2 signaling axis. PMID: 26809090
    7. The polycomb group protein L3MBTL1 represses a SMAD5-mediated hematopoietic transcriptional program in human pluripotent stem cells. PMID: 25754204
    8. among the 15 SNPs, rs3206634 was significantly associated with KD in a recessive model (odds ratio = 2.31, p = 0.019), whereas there was no association between any of the 15 SNPs and CALs. PMID: 24163009
    9. Specific gene siRNAs knock-down further confirmed the osteogenic effects of Genistein on BMP2, SMAD5 and RUNX2 protein expression PMID: 24339730
    10. ATP production by NaF promotes hypertrophy-like changes via activation of phospho-Smad5 PMID: 23384547
    11. found that restoration of SMAD5, in addition to the TGF-beta type II receptor, which was epigenetically silenced by the latent viral protein latency-associated nuclear antigen, sensitized BC3 cells to the cytostatic effect of TGF-beta signaling PMID: 22013049
    12. Studies indicate SMAD5beta isoform plays role in hematopoietic stem cell homeostasis. PMID: 20148926
    13. regulatory signals are active at transcriptionally subnuclear sites PMID: 12060751
    14. Smad5 activation by BMP4 in human hematopoietic cells results in significantly increased proliferation of erythroid progenitors and formation of glycophorin-A+ cells. PMID: 12064918
    15. up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection PMID: 12473652
    16. Results demonstrate a mitochondrial distribution of Smad5 in non-stimulated chondroprogenitor cells. PMID: 12849988
    17. SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma PMID: 14670176
    18. In mature human B cells, BMP-6 inhibited cell growth, and rapidly induced phosphorylation of Smad5. PMID: 15877825
    19. We used homology-modeling techniques to generate a reliable molecular model of the Smad5 MH1 domain based on the crystal structure of Smad3 MH1 domain PMID: 16243555
    20. SMAD5 gene was found to be associated with schizophrenia. PMID: 18298822

    顯示更多

    收起更多

  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • 蛋白家族：
    Dwarfin/SMAD family
  • 組織特異性：
    Ubiquitous.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6771

    OMIM: 603110

    KEGG: hsa:4090

    STRING: 9606.ENSP00000441954

    UniGene: Hs.167700