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  4. SIN3A Recombinant Monoclonal Antibody

SIN3A Recombinant Monoclonal Antibody

  • 中文名稱：
    SIN3A重組抗體
  • 貨號(hào)：
    CSB-RA242724A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate
      All lanes: mSin3A antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 146 kDa
      Observed band size: 146 kDa
    • IHC image of CSB-RA242724A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA242724A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of HepG2 Cells with CSB-RA242724A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    SIN3A Recombinant Monoclonal Antibody（Code: CSB-RA242724A0HU）是一款高特異性抗體，靶向SIN3A蛋白。SIN3A作為轉(zhuǎn)錄調(diào)控復(fù)合物的核心組分，通過組蛋白去乙?；揎梾⑴c染色質(zhì)結(jié)構(gòu)重塑及基因表達(dá)調(diào)控，在細(xì)胞周期、分化及凋亡等生物學(xué)過程中發(fā)揮重要作用。本抗體經(jīng)ELISA、蛋白質(zhì)印跡（WB）、免疫組織化學(xué)（IHC）和免疫熒光（IF）多平臺(tái)驗(yàn)證，表現(xiàn)出優(yōu)異的特異性和重復(fù)性。在WB實(shí)驗(yàn)中，其推薦稀釋比例為1:500-1:5000，可清晰識(shí)別目標(biāo)蛋白；IHC（1:50-1:200）和IF（1:20-1:200）實(shí)驗(yàn)顯示明確的亞細(xì)胞定位，適用于組織切片或細(xì)胞樣本中SIN3A的空間分布研究。該產(chǎn)品適用于探索基因表達(dá)調(diào)控機(jī)制、表觀遺傳學(xué)修飾及細(xì)胞命運(yùn)相關(guān)信號(hào)通路，為腫瘤發(fā)生、發(fā)育生物學(xué)和干細(xì)胞研究提供可靠工具。嚴(yán)格的質(zhì)量控制確保批次間穩(wěn)定性，滿足基礎(chǔ)科研中對(duì)蛋白功能分析和分子互作研究的需求。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Paired amphipathic helix protein Sin3a (Histone deacetylase complex subunit Sin3a) (Transcriptional corepressor Sin3a), SIN3A
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human mSin3A
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    10G3
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression. Required for cortical neuron differentiation and callosal axon elongation.
  • 基因功能參考文獻(xiàn)：
    1. AML patients with high SIN3 A expression levels had a worse prognosis than those with low expression. The same Y325Cvariant seen in endometrial carcinoma was found in one AML patient. It was calculated to cause a predicted stability change of -1.433 Kcal/mol, indicating significant disruption of the secondary structure. PMID: 29764005
    2. the FOXN3-NEAT1-SIN3A complex promotes epithelial-to-mesenchymal transition and invasion of breast cancer cells in vitro as well as dissemination and metastasis of breast cancer in vivo PMID: 28805661
    3. Haploinsufficiency of SIN3A causes mild intellectual disability by affecting the development of cortical integrity. PMID: 27399968
    4. Two novel translocations leading to the inactivation of RUNX1 and its partners SIN3A and TCF12 in myeloid leukemia. PMID: 26671595
    5. Study suggests a lack of association of SIN3A gene sequence variants with azoospermia caused by Sertoli cell-only syndrome in humans. PMID: 25395209
    6. down regulation of miR-202 increased the expression of its target Mxd1, followed by Mxd1 recruitment to the Sin3A repressor complex and through its dimerization with Max, and increased repression of Myc-Max target proteins. PMID: 25611699
    7. protein pairs significantly correlated with an increased risk of death in non-small cell lung cancer: mSin3A with p16, and c-Myc with mSinA PMID: 26118188
    8. Aberrantly expressed miR-210 regulates human U251 glioma cells apoptosis and proliferation partly through directly down-regulating SIN3A protein expression. PMID: 25481483
    9. Bone-marrow-specific deletion of Sin3a, indicated that Sin3a-associated HDAC1/2-activity is essential for hematopoietic stem cell homeostasis. PMID: 24763403
    10. SIN3A, generally regarded as a transcriptional repressor, is required for induction of gene transcription by the aryl hydrocarbon receptor. PMID: 25305016
    11. EBNA3C binds strongly to BATF/IRF4/SPI1/RUNX3 sites to enhance transcription and recruits RBPJ/Sin3A- and REST/NRSF-repressive complexes to repress p14(ARF) and p16(INK4A) expression PMID: 24344258
    12. reducing Sin3A strongly increased the invasive behavior of A549 human lung adenocarcinoma cells; findings show that Sin3A is downregulated in a variety of human tumors PMID: 22890320
    13. The Sin3a complex acts as a context-dependent ISGF3/STAT3 transcriptional switch. PMID: 22783022
    14. Ume6 is a negative regulator of ATG8 transcription, which acts along with a histone deacetylase complex including Sin3 and Rpd3 to regulate Atg8 levels PMID: 22733735
    15. Interplay between SIN3A and STAT3 mediates chromatin conformational changes and GFAP expression during cellular differentiation.( PMID: 21779366
    16. Sin3a, HDAC1, and YY1 are co-factors for Gon4l and that Gon4l may function as a platform for the assembly of complexes that regulate gene expression. PMID: 21454521
    17. The study identifies Sin3A as a regulator of gene expression, survival, and growth in ERalpha-positive breast cancer cells. PMID: 20920219
    18. sumoylation of ING2 enhances its binding to the Sin3A/HDAC complex and is required to regulate gene transcriptions PMID: 20676127
    19. HDAC-Sin3A complex regulates LHR Gene transcription PMID: 12091390
    20. Menin is important for recruiting an mSin3A-histone deacetylase complex to repress JunD transcriptional activity. PMID: 14559791
    21. MeCP2 acts as a corepressor of PU.1 probably due to facilitating complex formation with mSin3A and HDACs. PMID: 14647463
    22. transcription corepressor mSin3A interacts with SMN (Survival motor neuron) protein PMID: 14749338
    23. Results suggest that SHP mediates recruitment of mSin3A-Swi/Snf to the CYP7A1 promoter, resulting in chromatin remodeling and gene repression. PMID: 15314177
    24. p53 antagonizes c-Myb by recruiting mSin3A to down-regulate specific Myb target genes PMID: 15509555
    25. Data indicate that T-bet can override repressive epigenetic modification through a T-box half-site and dissociation of the mSin3a corepressor from the promoter. PMID: 15684083
    26. Sin3A-mediated deacetylation within the coding regions of active genes is directly linked to the histone methyltransferase activity of Smyd2 PMID: 16805913
    27. Sin3A-mediated deacetylation within the coding regions of active genes is directly linked to the histone methyltransferase activity of Smyd2 [Symd2] PMID: 16805913
    28. Repression of p53-mediated transcription by adenovirus E1B 55-kDa does not require corepressor mSin3A and histone deacetylases. PMID: 17209038
    29. the attenuated function of SIN3A due to a decreased level of expression may result in epigenetic de-regulation of growth-related genes through histone acetylation, which leads to the tumorigenesis of lung cancer cells. PMID: 17854949
    30. PRMT1- dependent methylation of RUNX1 at arginine residues 206 and 210 abrogates its association with SIN3A. PMID: 18316480
    31. A novel regulatory signaling mechanism of transcriptional control in which the LHR is derepressed through PKCalpha/ERK-mediated Sp1 phosphorylation, causing the release of HDAC1/mSin3A complex from the promoter. PMID: 18372343
    32. These data demonstrate that targeted degradation of NFX1-91 by E6/E6AP dissociates the mSin3A/HDAC complex from the hTERT promoter and induces hTERT transcription. PMID: 18505829
    33. Resullts show that SHP and Sin3A play an important role in adamantyl-substituted retinoid-related induction of cellular apoptosis. PMID: 19509248
    34. These data support a model of repression wherein actions of ERalpha and Sin3A at the proximal promoter can overcome activating signals at distal or proximal sites and ultimately decrease gene expression. PMID: 19620290
    35. The transcriptional repressor mSin3A associates with histone deacetylase 1 forming a co-repressor complex. See also PMID (PubMed identifier) 9150133. PMID: 9150134
    36. mSin3A interacts with HDAC2 via the HDAC-interacting domain (HID) of mSin3A. PMID: 9150134

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  • 亞細(xì)胞定位：
    Nucleus. Nucleus, nucleolus. Note=Recruited to the nucleolus by SAP30L.
  • 組織特異性：
    Expressed in the developing brain, with highest levels of expression detected in the ventricular zone of various cortical regions.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 19353

    OMIM: 607776

    KEGG: hsa:25942

    STRING: 9606.ENSP00000353622

    UniGene: Hs.513039