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SIRT5 Recombinant Monoclonal Antibody

  • 中文名稱(chēng)：
    SIRT5重組抗體
  • 貨號(hào)：
    CSB-RA156557A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA156557A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA156557A0HU SIRT5重組單克隆抗體是針對(duì)哺乳動(dòng)物去乙酰化酶SIRT5開(kāi)發(fā)的高特異性科研工具，該靶點(diǎn)作為sirtuin家族成員，通過(guò)調(diào)控線粒體蛋白的賴(lài)氨酸去琥珀酰化及戊二?；揎梾⑴c細(xì)胞代謝穩(wěn)態(tài)、能量平衡與氧化應(yīng)激反應(yīng)。經(jīng)嚴(yán)格驗(yàn)證，本抗體在免疫組織化學(xué)（IHC）實(shí)驗(yàn)中可清晰定位SIRT5蛋白于石蠟包埋組織樣本的亞細(xì)胞結(jié)構(gòu)，推薦工作濃度為1:50-1:200，同時(shí)在ELISA平臺(tái)展示出良好的線性結(jié)合特性。其表位特異性經(jīng)多批次抗原驗(yàn)證，背景信號(hào)低且批次穩(wěn)定性?xún)?yōu)異，適用于探究SIRT5在糖脂代謝異常、腫瘤微環(huán)境調(diào)控及神經(jīng)退行性疾病模型中的表達(dá)分布特征，可為代謝通路研究、疾病分子機(jī)制解析及小分子調(diào)節(jié)劑篩選提供可靠的蛋白檢測(cè)支持。該產(chǎn)品適用于基礎(chǔ)科研領(lǐng)域的組織切片分析、體外蛋白互作檢測(cè)及靶向治療相關(guān)生物標(biāo)記物探索。
  • Uniprot No.：
  • 基因名：
  • 別名：
    NAD-dependent protein deacylase sirtuin-5, mitochondrial (EC 3.5.1.-) (Regulatory protein SIR2 homolog 5) (SIR2-like protein 5), SIRT5, SIR2L5
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human SIRT5
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類(lèi)型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    9H12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
  • 基因功能參考文獻(xiàn)：
    1. In human beings, SIRT5 gene encodes for four SIRT5 protein isoforms, namely SIRT5iso1, SIRT5iso2, SIRT5iso3, and SIRT5iso4. SIRT5(iso1-3) were mitochondria-localized, while SIRT5(iso4) localized mainly in cytoplasm. SIRT5(iso2-4) had little deacylase activity comparing with SIRT5(iso1). PMID: 29932920
    2. miR-299-3p suppresses migration, invasion and proliferation of Hepatocellular carcinoma cells via directly targeting SIRT5 PMID: 30170358
    3. High SIRT5 expression is associated with Cancer Cell Proliferation. PMID: 29180469
    4. The DNA variants and SNPs identified in acute Myocardial infection (AMI) patients may change SIRT5 level by affecting activity of SIRT5 gene promoter, contributing to the AMI development as a risk factor. PMID: 29981421
    5. In this study, we summarize biological functions of SIRT5 reported in normal tissues and in cancer and discuss potential mechanisms whereby SIRT5 may impact tumorigenesis, particularly focusing on its reported roles in metabolic reprogramming. Finally, we review current efforts to target SIRT5 pharmacologically PMID: 28707979
    6. The roles of the CDK2/SIRT5 axis in gastric cancer. PMID: 29896817
    7. SIRT5 gene has an important regulatory role in liver carcinogenesis, and may function as a novel potential therapeutic target for HCC. PMID: 29115436
    8. This study showed that SIRT5 supports the anaplerotic entry of glutamine into the TCA cycle in malignant phenotypes of CRC via activating GLUD1. PMID: 29416026
    9. Results show that SIRT5 binds to, desuccinylates and inhibits PKM2 activity. Increased levels of reactive oxygen species (ROS) decreases succinylation and activity of PKM2 by increasing its binding to SIRT5. Moreover, inhibition of SIRT5 suppresses tumor cell proliferation through desuccinylation of PKM2 K498. PMID: 28036303
    10. The function of the three mitochondrial sirtuins (SIRT3, SIRT4, SIRT5) and their role in disease are reviewed. PMID: 27164052
    11. Our study uncovers a SIRT5-dependent mechanism that regulates cellular NADPH homeostasis and redox potential by promoting IDH2 desuccinylation and G6PD deglutarylation. PMID: 27113762
    12. Results demonstrated presence of endogenous SIRT5 in mitochondria of cultured SH-EP cells, identified down-regulation of cellular oxidative stress by SIRT5 as one of the possible mechanisms mediating the anti-apoptotic effect of SIRT5 in SH-EP cells. PMID: 27577743
    13. Data (including data from studies using knockout mice) suggest that SIRT5 is targeted to protein complexes on the inner mitochondrial membrane via affinity for cardiolipin to promote respiratory chain function, particularly Complex I and Complex II; SIRT5 expression is observed in inner mitochondrial membrane of periportal hepatocytes. PMID: 28458255
    14. a side chain-to-side chain cyclic pentapeptide harboring a central N(epsilon)-carboxyethyl-thiocarbamoyl-lysine residue behaved as a strong and selective (versus human SIRT1/2/3/6) inhibitor against human SIRT5-catalyzed deacylation reaction. PMID: 27626398
    15. mutual cooperation between Y102 and R105 residues in promoting the desuccinylation versus deacetylation reaction in SIRT5. PMID: 27023330
    16. Data showed that SIRT5 was involved in protein post-translational modifications through its potent demalonylase, desuccinylase, and deglutarylase activities. Also, the protein was found in the mitochondrial, cytoplasmic, and nuclear compartments. [review] PMID: 25717114
    17. Results show that mitochondrial sirtuins SIRT3, SIRT4, and SIRT5 can promote increased mitochondrial respiration and cellular metabolism and respond to excess glucose by inducing a coordinated increase of glycolysis and respiration. PMID: 25165814
    18. This study demonstrated that SIRT15 downregulation in the liver of non-alcoholic fatty liver disease patients. PMID: 25361925
    19. Results suggest a role for SIRT5 in influencing oocyte quality and in vitro fertilization outcomes. PMID: 23978077
    20. SIRT1/SIRT5-mediated PML deacetylation plays a role in the regulation of cancer cell survival. PMID: 25032863
    21. The results reveal the Sirt5 substrate acyl selectivity and its molecular basis and enable the design of inhibitors for Sirt5. PMID: 25111069
    22. High SIRT5 expression facilitates cancer cell growth and drug resistance in non-small cell lung cancer. PMID: 25070488
    23. Expression of SIRT5 in the entorhinal cortex, hippocampus, and white matter increases during the progression of Alzheimer disease. It is seen in activated microglia. PMID: 24464653
    24. These results reveal a novel post-translational regulation of SOD1 by means of succinylation and SIRT5-dependent desuccinylation, which is important for the growth of lung tumor cells. PMID: 24140062
    25. Downregulation of SIRT5 is associated with head and neck squamous cell carcinoma. PMID: 23475622
    26. Analysis of Sirt5 structures and activity data suggest that an Arg/succinate interaction is the molecular basis of the differential nicotinamide sensitivities of the two Sirt5 activities. PMID: 23028781
    27. The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5). PMID: 22767592
    28. NAD(+)-dependent SIRT deacetylase has a role in regulating the expression of mitochondrial steroidogenic P450 PMID: 22585829
    29. we demonstrate that Sirt5, a member of the class III lysine deacetylases, can catalyze lysine demalonylation and lysine desuccinylation reactions both in vitro and in vivo PMID: 21908771
    30. study found that Sirt5 is an efficient protein lysine desuccinylase and demalonylase in vitro; protein lysine succinylation may represent a posttranslational modification that can be reversed by Sirt5 in vivo PMID: 22076378
    31. SIRT5 represents a risk factor for mitochondrial dysfunction-related diseases, including Parkinson's, through accelerated molecular aging of disease-related genes. PMID: 20887790
    32. these results indicate that human SIRT5 potentially controls various primate-specific functions via two isoforms with different intracellular localizations or stabilities. PMID: 21143562
    33. SIRT5 consists of eight exons and is found in two isoforms, which encode a 310 aa and a 299 aa protein, respectively. PMID: 16484774
    34. Analyses did not yield convincing evidence for associations of schizophrenia with SIRT5. PMID: 16827919
    35. Results describe the substrates and regulation mechanisms for the human mitochondrial sirtuins Sirt3 and Sirt5. PMID: 18680753

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  • 亞細(xì)胞定位：
    Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Note=Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.; [Isoform 1]: Cytoplasm. Mitochondrion.; [Isoform 2]: Mitochondrion.
  • 蛋白家族：
    Sirtuin family, Class III subfamily
  • 組織特異性：
    Widely expressed.
  • 數(shù)據(jù)庫(kù)鏈接：

    HGNC: 14933

    OMIM: 604483

    KEGG: hsa:23408

    STRING: 9606.ENSP00000368552

    UniGene: Hs.567431