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SOX10 Recombinant Monoclonal Antibody

  • 中文名稱：
    SOX10重組抗體
  • 貨號(hào)：
    CSB-RA592917A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate
      All lanes: SOX10 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 50, 32 kDa
      Observed band size: 60 kDa
    • IHC image of CSB-RA592917A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA592917A0HU diluted at 1:100 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela Cells with CSB-RA592917A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA592917A0HU SOX10重組單克隆抗體是針對SRY相關(guān)HMG-box轉(zhuǎn)錄因子10開發(fā)的高特異性科研試劑，適用于多種實(shí)驗(yàn)場景。該抗體經(jīng)ELISA驗(yàn)證顯示優(yōu)異的抗原結(jié)合能力，在免疫印跡（WB）中能特異性識(shí)別天然及重組SOX10蛋白，并在人源細(xì)胞裂解液及組織樣本中檢測到預(yù)期條帶。其免疫組化（IHC）性能在石蠟包埋組織中表現(xiàn)出清晰的核定位染色，適用于腫瘤病理分析；免疫熒光（IF）實(shí)驗(yàn)顯示在細(xì)胞核內(nèi)呈現(xiàn)高信噪比的特異性信號(hào)。推薦使用稀釋度為WB 1:500-1:5000、IHC 1:50-1:200、IF 1:20-1:200。SOX10作為神經(jīng)嵴細(xì)胞分化和黑色素細(xì)胞發(fā)育的關(guān)鍵調(diào)控因子，該抗體可廣泛應(yīng)用于神經(jīng)發(fā)育研究、黑色素瘤機(jī)制探索以及雪旺細(xì)胞相關(guān)疾病模型中，特別適用于腫瘤微環(huán)境分析、細(xì)胞分化追蹤等基礎(chǔ)研究。重組單克隆抗體技術(shù)確保了批次間的高度一致性，為神經(jīng)生物學(xué)、腫瘤學(xué)等領(lǐng)域的蛋白表達(dá)研究和分子互作實(shí)驗(yàn)提供可靠工具。
  • Uniprot No.：
  • 基因名：
    SOX10
  • 別名：
    Transcription factor SOX-10, SOX10
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human SOX10
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號(hào)：
    8E1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, IHC, IF
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 儲(chǔ)存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Transcription factor that plays a central role in developing and mature glia. Specifically activates expression of myelin genes, during oligodendrocyte (OL) maturation, such as DUSP15 and MYRF, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Once induced, MYRF cooperates with SOX10 to implement the myelination program. Transcriptional activator of MITF, acting synergistically with PAX3. Transcriptional activator of MBP, via binding to the gene promoter.
  • 基因功能參考文獻(xiàn)：
    1. there have been a number of related reports that mutation of SOX10 will lead to Kallmann syndrome with deafness. PMID: 29726667
    2. phylogenetic analysis and three-dimensional modelling of the SOX10 protein confirmed that the c.1333delT heterozygous mutation was pathogenic, indicating that this mutation might constitute a candidate disease-causing mutation. PMID: 28128317
    3. Use of reliable positive and negative tissue controls is an important issue that must be addressed in any immunohistochemical staining reaction.4 SOX10 is a challenging marker in this sense, as no easy accessible tissues with consistent low-level expression have been identified at this time PMID: 28549040
    4. High SOX10 expression is associated with Basal Breast Cancers. PMID: 28216417
    5. Data show that depletion of SRY (sex determining region Y)-box 10 protein (SOX10) sensitizes mutant proto-oncogene proteins B-raf (BRAF) melanoma cells to RAF inhibitors in vitro and in vivo. PMID: 29295999
    6. SOX10 immunohistochemistry may be of utility in distinguishing some of the varying adnexal tumors from each other, and from basal cell carcinoma (BCC), but given the staining of both apocrine and eccrine tumors, does not seem to provide information as to their origins as either eccrine or apocrine tumors. PMID: 28343365
    7. Adenocarcinomas or adenomas derived from pigmented ciliary epithelium is distinguished from uveal melanoma by the absence of SOX10 expression and presence of the BRAF V600E mutation. PMID: 29059311
    8. Therefore, the mutant cannot transactivate the MITF promoter effectively, inhibiting melanin synthesis and leading to WS2. Our study confirmed haploinsufficiency as the underlying pathogenesis for WS2. PMID: 28893539
    9. Sox10 labeling is seen in a subset of metastatic triple-negative breast carcinomas, supporting its use as a marker of breast origin in this setting. PMID: 28843711
    10. SOX10 is useful in the differential diagnosis of salivary gland neoplasms. PMID: 27327192
    11. An extended immunohistochemical panel that includes beta-catenin and SOX10 helps to support the diagnosis of biphenotypic sinonasal sarcoma without the need for gene rearrangement studies. PMID: 27137987
    12. found that all SOX10-NL-positive cells expressed an early neural crest marker NGFR, however SOX10-NL-positive cells purified from differentiated hiPS cells progressively attenuate their NL-expression under proliferation PMID: 28107504
    13. The expression of the endogenous transcript is induced in a heterologous cell line by ectopically expressing SOX10, and is nearly ablated in Schwann cells by impairing SOX10 function. Intriguingly, overexpressing the two MTMR2 protein isoforms in HeLa cells revealed that both localize to nuclear puncta and the shorter isoform displays higher nuclear localization compared to the longer isoform PMID: 27466180
    14. This study assesses MYB, CD117 and SOX-10 expression in cutaneous adnexal tumors. PMID: 28098399
    15. Our zebrafish CHARGE model thus reveals important regulatory roles for Chd7 at multiple points of neural crest development viz., migration, fate choice and differentiation and we suggest that sox10 deregulation is an important driver of the neural crest-derived aspects of Chd7 dependent CHARGE syndrome. PMID: 27418670
    16. Data indicate that transcription factors Sox10 and Olig2 play key roles in oligodendrocytes (OLs) specification. PMID: 27785726
    17. SRY (sex determining region Y)-box 10 protein (SOX10) enhances nestin protein (NES) expression via directly binding to the promoter of NES. PMID: 28189679
    18. mutation is associated with distinctive phenotypic profile (association of anosmia and chronic constipation with SOX10 mutations) PMID: 28390600
    19. A low-level expression of Sox10 was significantly associated with high-level venous invasion by immunohistochemical evaluation, while it was significantly associated with high-level lymphatic permeation when analyzed by real-time PCR assay. PMID: 27943102
    20. SOX10 expression is elevated in serum of melanoma and vitiligo patients as compared to controls. PMID: 27110718
    21. Study provides evidence that the tumor suppressor Fbxw7alpha is the E3 ubiquitin ligase responsible for the degradation of SOX10, and suggests that reduced Fbxw7alpha might contribute to the upregulation of SOX10 in melanoma cells. PMID: 26461473
    22. Sox10 is expressed in many ovarian carcinomas PMID: 26951260
    23. we demonstrated that SOX10 is one of the most consistent markers of CD133+ stem-like ACC cells. Expression of SOX10 is also seen in other cancers, suggesting that they may contain similar stem-like cells. PMID: 27084744
    24. Despite the fact that the E248fs has a dominant-negative effect on SOX10, its reduced stability may down-regulate the transcription of MITF and decrease the synthesis of melanin PMID: 27454999
    25. SOX10-positivity rules out the diagnosis of ependymoma among other glial tumors with high confidence PMID: 26287936
    26. SOX-10 expression is exclusively specific for all cases of metastatic melanoma. PMID: 25611246
    27. This study demonstated that Shows no differences in expression level in ependymomas from Infants versus older children or amongiMolecular Subgroups. PMID: 26945037
    28. SOX10 mutations can mimic non-syndromic hearing impairment. PMID: 25256313
    29. our data imply that the same SOX10 mutations can underlie both typical Waardenburg syndrome and Kallmann Syndrome with deafness without skin/hair hypopigmentation, Hirschsprung disease, or neurological defects PMID: 26228106
    30. Subnuclear re-localization of SOX10 and p54NRB correlates with a unique neurological phenotype associated with SOX10 missense mutations. PMID: 26060192
    31. Our result confirm the thesis that heterozygous deletions at SOX10 is an important pathogenicity for Waardenburg syndrome type II. PMID: 26296878
    32. The use of SOX10 may increase the diagnostic accuracy of salivary gland oncocytic lesions on fine needle aspiration. PMID: 26619208
    33. Study shows that by uncoupling the effects of gain-of-function and haploinsufficiency in vivo, the effect of PCWH-causing SOX10 mutation is solely pathogenic in each SOX10-expressing cellular lineage in a dosage-dependent manner. PMID: 25959061
    34. Melanoma reprogramming involves thousands of genomic regulatory regions underlying the proliferative and invasive states, identifying SOX10/MITF and AP-1/TEAD as regulators, respectively. PMID: 25865119
    35. Identification of a rare dominant heterozygous SOX10 mutation c.621C>A in this family provided an efficient way to understand the causes of Waardenburg syndrome type II and improved genetic counseling. PMID: 25817900
    36. Sox10 is superior to S100 in the differential diagnosis of schwannoma and meningioma. PMID: 25265429
    37. reliable marker for detecting metastatic melanoma in sentinel lymph nodes PMID: 25356946
    38. Loss of SOX10 is associated with digestive cancers. PMID: 25301735
    39. we examined Sox10 expression in 5134 human neoplasms spanning a wide spectrum of neuroectodermal, mesenchymal, lymphoid, and epithelial tumors. PMID: 25724000
    40. Results show that SRY could be expressed in tissues of Hirschsprung patients. It binds to the promoter of RET gene by competing with SOX10 for its interaction with PAX3 and NKX2-1 repressing their transcriptional expression and RET's as well. PMID: 25267720
    41. Data indicate that SOX transcription factor SOX10 was expressed in 238 of 257 melanomas, including 50 of 51 of both spindle cell and desmoplastic melanomas. PMID: 25436903
    42. SH3TC2 is regulated by the transcription factors CREB and SOX10, define a regulatory SNP at this disease-associated locus and reveal SH3TC2 as a candidate modifier locus of CMT disease phenotypes. PMID: 24833716
    43. High SOX10 expression is associated with gangliocytic paraganglioma. PMID: 25562414
    44. This study reports on three independent families with SOX10 mutations predicted to result in the same missense mutation at the protein level. PMID: 24845202
    45. Sox10 (and Sox2) activate transcriptional elongation in Schwann cells by recruiting the positive transcription elongation factor b. PMID: 25524031
    46. Decreases in Sox10 expression levels and a loss of Sox10(+) cells in both mouse and human aged ears suggests an important role of Sox10 in the maintenance of structural and functional integrity of the lateral wall. PMID: 24887110
    47. haploinsufficiency of SOX10 may "unmask" subtler effects on expression or epistasis associated with variants in SOX10 targets (e.g., DHH), in its partners (e.g., PAX3, EGR2), and in genes with functional overlap (e.g., SOX8, SOX9). PMID: 24715709
    48. The novel heterozygous c.259-260delCT mutation in the SOX10 gene was considered to be the cause of Waardenburg syndrome PMID: 24735604
    49. SOX10 facilitates TCF4 to bind to beta-catenin and form a stable SOX10/TCF4/beta-catenin complex and trans-activate its downstream target gene in human hepatocellular carcinoma PMID: 25001176
    50. SOX-10 is a relatively reliable marker for staining cutaneous myoepitheliomas. PMID: 24329979

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  • 相關(guān)疾?。?/div>
    Waardenburg syndrome 2E (WS2E); Waardenburg syndrome 4C (WS4C); Peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome and Hirschsprung disease (PCWH)
  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side.
  • 組織特異性：
    Expressed in fetal brain and in adult brain, heart, small intestine and colon.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 11190

    OMIM: 602229

    KEGG: hsa:6663

    STRING: 9606.ENSP00000354130

    UniGene: Hs.376984