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  4. Recombinant 3'-5' exonuclease eri-1 (eri-1), partial

Recombinant 3'-5' exonuclease eri-1 (eri-1), partial

  • 中文名稱(chēng)：
    秀麗隱桿線蟲(chóng)eri-1重組蛋白
  • 貨號(hào)：
    CSB-YP523685CXY
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    秀麗隱桿線蟲(chóng)eri-1重組蛋白
  • 貨號(hào)：
    CSB-EP523685CXY
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    秀麗隱桿線蟲(chóng)eri-1重組蛋白
  • 貨號(hào)：
    CSB-EP523685CXY-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    秀麗隱桿線蟲(chóng)eri-1重組蛋白
  • 貨號(hào)：
    CSB-BP523685CXY
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    秀麗隱桿線蟲(chóng)eri-1重組蛋白
  • 貨號(hào)：
    CSB-MP523685CXY
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    eri-1
  • Uniprot No.：
  • 別名：
    eri-1; T07A9.53'-5' exonuclease eri-1; EC 3.1.-.-; Enhanced RNAi protein
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    RNA exonuclease that acts as a negative regulator of RNA interference (RNAi). Probably acts by degrading the 3'-overhangs of short interfering RNAs (siRNAs). Component of the ERI/DICER complex which is involved in processing amplified double-stranded RNA (dsRNA) intermediates during small-RNA-mediated gene-silencing or RNA interference (RNAi) (Probable).
  • 基因功能參考文獻(xiàn)：
    1. In the absence of eri-1, reserpine mediated lifespan extension (RMLE) is shortened. In the dop-3 loss-of-function background, lack of eri-1 completely abolishes RMLE. This suggests that dop-3 and eri-1 act in independent parallel pathways for RMLE and these two pathways are essential and sufficient for the longevity enhancement by reserpine in C. elegans. PMID: 27966489
    2. results identify novel components of the endo RNAi machinery, demonstrate differential requirements for the Eri factors in the sperm-producing germline, and begin to delineate the functional requirement for the ERI/DICER complex in sperm development PMID: 19797044
    3. After exposure to dsRNA or siRNAs, animals with eri-1 mutations accumulate more siRNAs than wild-type animals; a negative regulator that may normally function to limit the duration, cell-type specificity or endogenous functions of RNA interference PMID: 14961122
    4. Feeding unc-13 & eri-1 strains dsRNA corresponding to a neuronally expressed GFP reporter resulted in reduction of GFP in double mutants compared to GFP expression in eri-1 mutants, indicating that UNC-13 functions in conjunction with ERI-1 in RNAi. PMID: 17276405
    5. In C. elegans, two protein isoforms of ERI-1 are localized to the cytoplasm, and each has distinct functions in ribosomal RNA processing and negative regulation of RNA interference. PMID: 18438419

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  • 亞細(xì)胞定位：
    Cytoplasm.
  • 組織特異性：
    Expressed in the somatic developing gonads and in a subset of neurons. In adults, it is specifically expressed in neurons and in spermatheca.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: cel:CELE_T07A9.5

    STRING: 6239.T07A9.5b

    UniGene: Cel.12380