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  4. Recombinant Anillin-like protein 1 (ani-1), partial

Recombinant Anillin-like protein 1 (ani-1), partial

  • 中文名稱：
    Recombinant Anillin-like protein 1(ani-1) ,partial
  • 貨號：
    CSB-YP893178CXY
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Anillin-like protein 1(ani-1) ,partial
  • 貨號：
    CSB-EP893178CXY
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Anillin-like protein 1(ani-1) ,partial
  • 貨號：
    CSB-EP893178CXY-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Anillin-like protein 1(ani-1) ,partial
  • 貨號：
    CSB-BP893178CXY
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Anillin-like protein 1(ani-1) ,partial
  • 貨號：
    CSB-MP893178CXY
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ani-1
  • Uniprot No.：
  • 別名：
    ani-1; Y49E10.19Anillin-like protein 1
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Required for contractile events in embryos that occur prior to mitosis, such as cortical ruffling and pseudocleavage. Promotes membrane ruffling by organizing cortical patches of septins and myosin II. Not generally required for cytokinesis in mitotic cells. Required for the asymmetric cleavage events that extrude the two polar bodies during oocyte meiosis. Not required for meiotic contractile ring assembly, initiation or closure but is required for the transformation of the contractile ring from a disk above the spindle to a tube around the spindle midzone. Promotes astral microtubule-directed cortical myosin polarization and cleavage furrow ingression. Regulates neuroblast cytokinesis during mid- to late-embryogenesis and is required for ventral enclosure.
  • 基因功能參考文獻：
    1. ANI-1 and ANI-2 contribute to embryonic viability by performing distinct functions in the early embryo and gonad, respectively [ANI-1 & ANI-2] PMID: 15930113
    2. active form of RhoG/MIG-2 directly binds to Anillin and recruits it to the leading edge of Q neuroblasts PMID: 25843030
    3. Caenorhabditis elegans anillin (ani-1) regulates neuroblast cytokinesis and epidermal morphogenesis during embryonic development. PMID: 24016757
    4. Anillin-1 promotes astral microtubule-directed cortical myosin polarization PMID: 21737681
    5. Anillin is dispensable for contractile ring assembly, initiation, and closure but is required for the meiotic contractile ring to transform from a disc into a tube. PMID: 21055941
    6. Asymmetric ingression requires Anillin and the septins, which promote the coalescence of components on one side of the contractile ring, but is insensitive to a 10-fold reduction in myosin II levels. PMID: 17488632

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  • 亞細胞定位：
    Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Midbody. Cleavage furrow.
  • 組織特異性：
    Strongly expressed in dividing neuroblasts under the ventral epidermal cells during ventral enclosure.
  • 數(shù)據(jù)庫鏈接：