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  4. Recombinant Arabidopsis thaliana 3-isopropylmalate dehydrogenase 3, chloroplastic (IMDH3)

Recombinant Arabidopsis thaliana 3-isopropylmalate dehydrogenase 3, chloroplastic (IMDH3)

  • 中文名稱：
    擬南芥IMDH3重組蛋白
  • 貨號(hào)：
    CSB-YP887816DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥IMDH3重組蛋白
  • 貨號(hào)：
    CSB-EP887816DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥IMDH3重組蛋白
  • 貨號(hào)：
    CSB-EP887816DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥IMDH3重組蛋白
  • 貨號(hào)：
    CSB-BP887816DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥IMDH3重組蛋白
  • 貨號(hào)：
    CSB-MP887816DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    IMDH3
  • Uniprot No.：
  • 別名：
    IMDH1; IMD1; IPMDH1; MAM-D1; At5g14200; MUA22.203-isopropylmalate dehydrogenase 1; chloroplastic; 3-IPM-DH 1; AtIMDH1; IMDH 1; EC 1.1.1.85; Beta-IPM dehydrogenase 1; Isopropylmalate dehydrogenase 1; AtIMD1; Methylthioalkylmalate dehydrogenase 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    38-409
  • 氨基酸序列
    AAA SPGKKRYNIA LLPGDGIGPE VISVAKNVLQ KAGSLEGLEF DFKEMPVGGA ALDLVGVPLP EETFTAAKLS DAILLGAIGG YKWDKNEKHL RPEMALFYLR RDLKVFANLR PATVLPQLVD ASTLKKEVAE GVDMMIVREL TGGIYFGEPR GITINENGEE VGVSTEIYAA HEIDRIARVA FETARKRRGK LCSVDKANVL DASILWRKRV TALASEYPDV ELSHMYVDNA AMQLIRDPKQ FDTIVTNNIF GDILSDEASM ITGSIGMLPS ASLGESGPGL FEPIHGSAPD IAGQDKANPL ATILSAAMLL KYGLGEEKAA KRIEDAVVDA LNKGFRTGDI YSPGNKLVGC KEMGEEVLKS VESKVPATV
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in both glucosinolate and leucine biosynthesis; catalyzes the oxidative decarboxylation step in both leucine biosynthesis (primary metabolism) and methionine chain elongation of glucosinolates (specialized metabolism). Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate, 3-IPM) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. Required during pollen development and involved in embryo sac development. More active on 3-isopropylmalate and NAD(+) than towards D-malate.
  • 基因功能參考文獻(xiàn)：
    1. AtLeuC1 and AtIMD1 encode methylthioalkylmalate isomerase and methylthioalkylmalate dehydrogenase, respectively. These enzymes have roles in controlling metabolism from methionine to primary and methionine-related secondary metabolites. [AtIMD1] PMID: 19493961
    2. Disruption of AtIPMDH1 in Arabidopsis caused a dramatic decrease in the concentrations of glucosinolates with side chains of four or more carbons, and a decrease in free leucine levels. PMID: 19674406
    3. IPMDH is involved in leucine biosynthetic pathway. Three IPMDH ORF sequences designated as AtIMD1, AtIMD2, and AtIMD3 are localized in plastids. AtIMD1 transcripts were the highest in roots. [AtIMD1; IPMDH] PMID: 15849421
  • 亞細(xì)胞定位：
    Plastid, chloroplast stroma.
  • 蛋白家族：
    Isocitrate and isopropylmalate dehydrogenases family
  • 組織特異性：
    Highly expressed in seedlings, leaves, stems and roots and, to a lower extent, in flowers, pollen and siliques.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT5G14200

    STRING: 3702.AT5G14200.1

    UniGene: At.23937