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  4. Recombinant Arabidopsis thaliana 65-kDa microtubule-associated protein 1 (MAP65-1), partial

Recombinant Arabidopsis thaliana 65-kDa microtubule-associated protein 1 (MAP65-1), partial

  • 中文名稱：
    擬南芥MAP65-1重組蛋白
  • 貨號(hào)：
    CSB-YP866974DOA
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥MAP65-1重組蛋白
  • 貨號(hào)：
    CSB-EP866974DOA
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥MAP65-1重組蛋白
  • 貨號(hào)：
    CSB-EP866974DOA-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥MAP65-1重組蛋白
  • 貨號(hào)：
    CSB-BP866974DOA
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥MAP65-1重組蛋白
  • 貨號(hào)：
    CSB-MP866974DOA
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MAP65-1
  • Uniprot No.：
  • 別名：
    MAP65-1; At5g55230; MCO15.18; 65-kDa microtubule-associated protein 1; AtMAP65-1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Microtubule-associated protein that bundle and stabilize adjacent microtubules (MT) of the cell cortex. Enhances MT nucleation. Can also bind to tubulin dimers and promotes their polymerization. Confers MT resistance to the drug propyzamide and cold conditions. Plays a role in the central spindle at anaphase to early cytokinesis but is not essential at the midline of the phragmoplast at later stages. Represses metaphase spindle organization and the transition to anaphase in dephosphorylated active form. Promotes the formation of a planar network of antiparallel microtubules. May be involved in stomatal movement modulation by regulating the dynamic and arrangement of cortical MT.
  • 基因功能參考文獻(xiàn)：
    1. MAP65-1 plays a critical role in the response to salt stress and is required for regulating the rapid depolymerization and reorganization of cortical microtubules (MTs). PMID: 28969791
    2. MAP65-1 is subject to multiple phosphorylation events by Aurora kinases during consecutive stages of the cell cycle. PMID: 27879390
    3. MAP65-1 and MAP65-2 play a critical role in the microtubule-dependent mechanism for specifying axial cell growth in the expanding hypocotyl, independent of any mechanical role in microtubule array organization. PMID: 21551389
    4. Results also show that MAP65-1 and MAP65-3 may share redundant functions in MT stabilization. PMID: 22570443
    5. the rod domain of MAP65-1 acts both as a spacer and as a structural element that specifies the microtubule encounter angles that are conducive for bundling PMID: 22385851
    6. AtMAP65-1 plays a role in the central spindle at anaphase to early cytokinesis but is not essential at the midline of the phragmoplast at later stages. PMID: 16098102
    7. Data suggest that normal metaphase spindle organisation and the transition to anaphase is dependent on inactivation of AtMAP65-1 PMID: 16847052
    8. AtMAP65-1 might promote tubulin assembly by binding to tubulin dimers in vivo.[AtMAP65-1] PMID: 17394772
    9. The predicted three-dimensional structure of AtMAP65-1 was used as a basis for analyzing the actual cross-bridging with microtubules in detail. PMID: 17504815
    10. MAP65-1 binds cortical microtubules. PMID: 17674780
    11. AtMAP65-1 and AtMAP65-5 promote antiparallel microtubule bundling PMID: 18667529

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Cytoplasm, cell cortex. Note=During interphase, binds cortical microtubules. In M-phase, locates to the preprophase band. Binds the preprophase band but does not accumulate at the prophase spindle microtubules that coexists within the same cell. In the metaphase and anaphase, localized to spindle. In the phragmoplast, concentrated at the midzone where vesicles guide by MT coalesce to form the cell plate. Present in MT cortical arrays. Concentrated in dots surrounding the nuclei.
  • 蛋白家族：
    MAP65/ASE1 family
  • 組織特異性：
    Expressed in all organs and tissues with the exception of sepals and anthers. Bound to subsets of microtubules in the cells of root epidermis, hypocotyl and cotyledons (at protein level).
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT5G55230

    STRING: 3702.AT5G55230.2

    UniGene: At.7440