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  4. Recombinant Arabidopsis thaliana DNA-directed RNA polymerase E subunit 1 (NRPE1), partial

Recombinant Arabidopsis thaliana DNA-directed RNA polymerase E subunit 1 (NRPE1), partial

  • 中文名稱：
    擬南芥NRPE1重組蛋白
  • 貨號：
    CSB-YP710698DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥NRPE1重組蛋白
  • 貨號：
    CSB-EP710698DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥NRPE1重組蛋白
  • 貨號：
    CSB-EP710698DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥NRPE1重組蛋白
  • 貨號：
    CSB-BP710698DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥NRPE1重組蛋白
  • 貨號：
    CSB-MP710698DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NRPE1
  • Uniprot No.：
  • 別名：
    NRPE1; DMS5; DRD3; NRPD1b; RMD1; RPE1; At2g40030; T28M21.19; DNA-directed RNA polymerase V subunit 1; DNA-directed RNA polymerase D subunit 1b; AtNRPD1b; Nuclear RNA polymerase D 1b; DNA-directed RNA polymerase E subunit 1; Nuclear RNA polymerase E 1; EC 2.7.7.6; Protein DEFECTIVE IN MERISTEM SILENCING 5; Protein DEFECTIVE IN RNA-DIRECTED DNA METHYLATION 3; Protein RNA-DIRECTED DNA METHYLATION DEFECTIVE 1; RNA polymerase IV subunit 1; POL IV 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase V involved in RNA-directed DNA methylation-dependent (RdDM) silencing of endogenous repeated sequences, including transposable elements. Also required for full erasure of methylation when the RNA trigger is withdrawn. Seems also involved in the synthesis of short-interfering RNAs (siRNA). Essential component of a self-reinforcing loop coupling de novo DNA methylation to siRNA production. Involved in the maintenance of post-transcriptional RNA silencing.
  • 基因功能參考文獻：
    1. DRD1-Pol V-dependent de novo methylation may play a role in restraining the inappropriate silencing of active protein-coding genes in plants. [NRPE1] PMID: 21771120
    2. Mutants defective for the NRPD1a and NRPD1b alternative largest subunits of polymerase IV were tested for their ability to undergo RNA silencing and mantenance of transcription and post-transcriptional silencing. PMID: 18433438
  • 亞細胞定位：
    Nucleus, nucleolus. Note=Clustered within heterochromatic regions and colocalized with RDR2, DCL3, NRPD4, AGO4, and siRNAs within the nucleolus. Recruited to chromatin via its interaction with SUVH2.
  • 蛋白家族：
    RNA polymerase beta' chain family
  • 組織特異性：
    Mostly expressed in flowers, and, to a lower extent, in leaves. Present in sperm cells.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT2G40030

    STRING: 3702.AT2G40030.1

    UniGene: At.37106