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  4. Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ORTHRUS 2 (ORTH2), partial

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase ORTHRUS 2 (ORTH2), partial

  • 中文名稱：
    擬南芥ORTH2重組蛋白
  • 貨號(hào)：
    CSB-YP840910DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥ORTH2重組蛋白
  • 貨號(hào)：
    CSB-EP840910DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥ORTH2重組蛋白
  • 貨號(hào)：
    CSB-EP840910DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥ORTH2重組蛋白
  • 貨號(hào)：
    CSB-BP840910DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥ORTH2重組蛋白
  • 貨號(hào)：
    CSB-MP840910DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ORTH2
  • Uniprot No.：
  • 別名：
    ORTH2; VIM1; At1g57820; F12K22.14; E3 ubiquitin-protein ligase ORTHRUS 2; EC 2.3.2.27; Protein VARIANT IN METHYLATION 1; RING-type E3 ubiquitin transferase ORTHRUS 2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    E3 ubiquitin-protein ligase. Participates in CpG methylation-dependent transcriptional regulation and epigenetic transcriptional silencing. Mediates ubiquitination with the E2 ubiquitin-conjugating enzyme UBC11. Promotes methylation-mediated gene silencing leading, for example, to early flowering. Associates with methylated DNA, and can bind to CpG, CpNpG, and CpNpN DNA motifs, with a strong preference for methylated forms, and with highest affinity for CpG substrate. Probably acts at the DNA methylation?histone interface to maintain centromeric heterochromatin.
  • 基因功能參考文獻(xiàn)：
    1. Observed full-length VIM1 binds preferentially to hemi-methylated DNA with a single modified 5mCpG site. However, when 5hmC replaces one or both cytosine residues at a palindromic CpG site, VIM1 binds with approximately >/=10-fold lower affinity. PMID: 22459921
    2. findings suggest that VIM1 (AT1G57820) acts at the DNA methylation-histone interface to maintain centromeric heterochromatin PMID: 17242155
  • 亞細(xì)胞定位：
    Nucleus. Note=Broadly distributed in the nucleus and enriched in the heterochromatic chromocenters.
  • 組織特異性：
    Mostly expressed in inflorescence and, to a lower extent, in leaves.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT1G57820

    STRING: 3702.AT1G57820.1

    UniGene: At.28484