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  4. Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 2 (At3g03300), partial

Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 2 (At3g03300), partial

  • 中文名稱：
    Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 2(At3g03300) ,partial
  • 貨號(hào)：
    CSB-YP659058DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 2(At3g03300) ,partial
  • 貨號(hào)：
    CSB-EP659058DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 2(At3g03300) ,partial
  • 貨號(hào)：
    CSB-EP659058DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 2(At3g03300) ,partial
  • 貨號(hào)：
    CSB-BP659058DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Endoribonuclease Dicer homolog 2(At3g03300) ,partial
  • 貨號(hào)：
    CSB-MP659058DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    At3g03300
  • Uniprot No.：
  • 別名：
    At3g03300; T17B22.1Endoribonuclease Dicer homolog 2; EC 3.1.26.-; Dicer-like protein 2; AtDCL2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Involved in the processing of natural small interfering RNAs (nat-siRNAs, derived from cis-natural antisense transcripts) by cleaving small dsRNAs into 24 nucleotide nat-siRNAs. Plays an essential role in transitive silencing of transgenes by processing secondary siRNAs. This pathway, which requires DCL4 and RDR6, amplifies silencing by using the target RNA as substrate to generate secondary siRNAs, providing an efficient mechanism for long-distance silencing. May participate with DCL3 in the production of 24 nucleotide repeat-associated siRNAs (ra-siRNAs) which derive from heterochromatin and DNA repeats such as transposons. Plays a role in antiviral RNA silencing. Involved in the production of viral siRNAs derived from the turnip crinkle virus (TCV) and tobacco rattle virus (TRV). Targeted by the viral silencing suppressor (VSR) protein 2b of the cucumber mosaic virus (CMV) that inactivates DCL2 function in RNA silencing. Does not seem to be involved in microRNAs (miRNAs) processing.
  • 基因功能參考文獻(xiàn)：
    1. DCL2 is crucial for RDR6-dependent systemic Posttranscriptional gene silencing. PMID: 28928141
    2. DCL2- and RDR6-dependent transitive silencing of SMXL4 and SMXL5 in Arabidopsis dcl4 mutants causes defective phloem transport and carbohydrate over-accumulation PMID: 28267232
    3. DCL2/4-dependent and -independent RNA silencing pathways are involved, and may crosstalk with basal innate immunity pathways, in host defense and in cross-protection. PMID: 24804808
    4. AGO2 and HEN1 participate in the DCL2-mediated antiviral defense to ensure the survival of Turnip crinkle virus-infected plants at high temperature. PMID: 22496240
    5. Data revealed that AtDCL2 is also preferentially expressed in the Arabidopsis egg cell. PMID: 21673515
    6. DCL4 and DCL2 exhibit specific, hierarchical antiviral activities; inhibition of DCL4 by a viral-encoded suppressor revealed the subordinate antiviral activity of DCL2 PMID: 16741077
    7. DCL2 antagonizes the production of miRNAs and siRNAs by DCL1 in certain circumstances and can substitute for DCL4 when DCL4 activity is reduced or inhibited by viruses. PMID: 16810317
    8. Study shows that unidirectional (3-->5') transitivity requires the hierarchical and redundant functions of DCL4 and DCL2 acting downstream from RDR6 to produce 21- and 22-nt-long siRNAs, respectively. PMID: 17592042
    9. DCL2 plays an essential, as opposed to redundant, role in transitive silencing of transgenes and may play a more important role in silencing of viruses than currently thought PMID: 18335032
    10. Data describe three alternative splicing (AS) forms of AtDCL2: one with both intron 8 and intron 10 are retained, second with only intron 8 is retained and third with no intron is retained, but there is a deletion of 56 nucleotides at the end of exon 10. PMID: 19011859
    11. The results reveal that dcl2, dcl3 and dcl4 mutations similarly affect post-transcriptional gene silencing (PTGS) mediated by a sense transgene and PTGS mediated by inverted repeats. PMID: 19343050
    12. Cross-protection by Fny-CMV2b against Fny-CMV infection was not abolished in dcl2, or dcl4 mutant plants. PMID: 19474248
    13. We have demonstrated that DCLs 2, 3 and 4 are dispensable for Salicylic acid-induced resistance to Tobacco Mosaic Virus and Cucumber Mosaic Virus. PMID: 19710258

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  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Helicase family, Dicer subfamily
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT3G03300

    STRING: 3702.AT3G03300.1

    UniGene: At.43488