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  4. Recombinant Arabidopsis thaliana Mitotic spindle checkpoint protein MAD2 (MAD2)

Recombinant Arabidopsis thaliana Mitotic spindle checkpoint protein MAD2 (MAD2)

  • 中文名稱(chēng)：
    擬南芥MAD2重組蛋白
  • 貨號(hào)：
    CSB-YP881819DOA
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱(chēng)：
    擬南芥MAD2重組蛋白
  • 貨號(hào)：
    CSB-EP881819DOA
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱(chēng)：
    擬南芥MAD2重組蛋白
  • 貨號(hào)：
    CSB-EP881819DOA-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱(chēng)：
    擬南芥MAD2重組蛋白
  • 貨號(hào)：
    CSB-BP881819DOA
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱(chēng)：
    擬南芥MAD2重組蛋白
  • 貨號(hào)：
    CSB-MP881819DOA
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MAD2
  • Uniprot No.：
  • 別名：
    MAD2; At3g25980; MPE11.15Mitotic spindle checkpoint protein MAD2; Mitotic arrest deficient protein 2
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-209
  • 氨基酸序列
    MASKTAAAKD IITLHGSAAI VSEFFCYAAN SILYNRAVYP EESFVKVKKY GLPMLLIEDE SVKSFMSNLT SQISEWLEAG KLQRVVLVIM SKATGEVLER WNFRIETDNE VVDKGVSREK SDKEIMREIQ AIMRQVASSV TYLPCLDETC VFDVLAYTDT DVAVPFTWIE SDPKLIANPQ MVKLHGFDTK IHKVDTLVSY KNDEWDEEE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. It inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that NUA scaffolds AtMAD1 and AtMAD2 at the nuclear pore to form a functional complex and that both NUA and AtMAD2 suppress premature exit from cell division at the Arabidopsis root meristem. PMID: 22457071
  • 亞細(xì)胞定位：
    Nucleus. Nucleus envelope. Chromosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cytoplasm.
  • 蛋白家族：
    MAD2 family
  • 組織特異性：
    Expressed in actively dividing tissues, early in organ development, in young leaves, lateral root primordia and root meristems.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT3G25980

    STRING: 3702.AT3G25980.1

    UniGene: At.37293