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  4. Recombinant Arabidopsis thaliana Nucleoside diphosphate kinase II, chloroplastic (NDPK2)

Recombinant Arabidopsis thaliana Nucleoside diphosphate kinase II, chloroplastic (NDPK2)

  • 中文名稱：
    擬南芥NDPK2重組蛋白
  • 貨號：
    CSB-YP524048DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥NDPK2重組蛋白
  • 貨號：
    CSB-EP524048DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥NDPK2重組蛋白
  • 貨號：
    CSB-EP524048DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥NDPK2重組蛋白
  • 貨號：
    CSB-BP524048DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥NDPK2重組蛋白
  • 貨號：
    CSB-MP524048DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    NDPK2
  • Uniprot No.：
  • 別名：
    NDPK2; At5g63310; MDC12.28; Nucleoside diphosphate kinase II; chloroplastic; NDK II; NDP kinase II; NDPK II; NDPK Ia; EC 2.7.4.6
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    63-231
  • 氨基酸序列
    ASSSAESG IFLPHLVASM EDVEETYIMV KPDGIQRGLV GEIISRFEKK GFKLIGLKMF QCPKELAEEH YKDLSAKSFF PNLIEYITSG PVVCMAWEGV GVVASARKLI GKTDPLQAEP GTIRGDLAVQ TGRNIVHGSD SPENGKREIG LWFKEGELCK WDSALATWLR E
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. May activate MPK3 and MPK6. May be involved in the regulation of cellular redox state and hydrogen peroxide-mediated MAP kinase signaling.
  • 基因功能參考文獻：
    1. These results suggest that enhanced AtNDPK2 expression affects oxidative stress tolerance leading to improved plant growth in transgenic poplar. PMID: 20649941
    2. Data suggest that binding of nucleotide to nucleoside diphosphate kinase 2 (NDPK2) plays a regulatory role in phytochrome signaling and that the C-terminal extension of NDPK2 provides a potential binding surface for interaction with phytochromes. PMID: 15465053
    3. characterization of the interaction between phytochrome and NDPK2 PMID: 15561724
    4. localizes only to chloroplasts, not to the cytosol PMID: 17562072
    5. NDPK2 plays a critical role in the AtPP7 regulation of the phytochrome pathway PMID: 18628957
    6. Results suggest that nucleoside diphosphate kinase 2 acts as a GTPase-activating protein for small G proteins in plants. PMID: 18823588

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  • 亞細胞定位：
    Plastid, chloroplast.
  • 蛋白家族：
    NDK family
  • 數據庫鏈接：

    KEGG: ath:AT5G63310

    STRING: 3702.AT5G63310.1

    UniGene: At.24504