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  4. Recombinant Arabidopsis thaliana Protein SUPPRESSOR OF GENE SILENCING 3 (SGS3), partial

Recombinant Arabidopsis thaliana Protein SUPPRESSOR OF GENE SILENCING 3 (SGS3), partial

  • 中文名稱：
    擬南芥SGS3重組蛋白
  • 貨號：
    CSB-YP878471DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥SGS3重組蛋白
  • 貨號：
    CSB-EP878471DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥SGS3重組蛋白
  • 貨號：
    CSB-EP878471DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥SGS3重組蛋白
  • 貨號：
    CSB-BP878471DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥SGS3重組蛋白
  • 貨號：
    CSB-MP878471DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SGS3
  • Uniprot No.：
  • 別名：
    SGS3; At5g23570; MQM1.17; Protein SUPPRESSOR OF GENE SILENCING 3; AtSGS3
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Required for post-transcriptional gene silencing and natural virus resistance. May bind nucleic acids and is essential for the biogenesis of trans-acting siRNAs but is not required for silencing induced by IR-PTGS. Involved in the juvenile-to-adult transition regulation. In case of begomoviruses infection, it is targeted by the viral protein V2 leading to suppression of post-transcriptional gene silencing.
  • 基因功能參考文獻：
    1. Viral genome-linked protein (VPg) acts as a suppressor of RNA silencing and suggest that an interaction with SGS3 may be important, especially in suppression of sense-mediated RNA silencing. PMID: 25099340
    2. Temperature increase reduces the protein abundance of SUPPRESSOR OF GENE SILENCING 3 and induce transgenerational epigenetic release of RNA silencing by inhibiting siRNA biogenesis in Arabidopsis. PMID: 23686579
    3. SGS3 associates with AGO1-RISC via the double-stranded RNA formed by the 3'-terminal nucleotides of 22-nt miR173 and corresponding target RNA, which probably protrudes from the AGO1-RISC molecular surface PMID: 23417299
    4. Components of the RNA-silencing machinery, RNA-DEPENDENT RNA POLYMERASE1 and SUPPRESSOR OF GENE SILENCING3 were isolated, implicating RNA silencing in the control of cuticular wax deposition during inflorescence stem development in Arabidopsis . PMID: 22689894
    5. Null sgs3 mutants lack TAS1, TAS2, and TAS3 ta-siRNAs and overaccumulate ARF3/ETTIN and ARF4 mRNAs, which are TAS3 ta-siRNA targets. PMID: 16682354
    6. sgs3-3 is a neomorphic allele that shows increased RNA protective activity PMID: 19143842

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  • 亞細胞定位：
    Cytoplasm, perinuclear region.
  • 蛋白家族：
    SGS3 family
  • 數(shù)據庫鏈接：

    KEGG: ath:AT5G23570

    STRING: 3702.AT5G23570.1

    UniGene: At.31001