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  4. Recombinant Arabidopsis thaliana Protein argonaute 10 (AGO10), partial

Recombinant Arabidopsis thaliana Protein argonaute 10 (AGO10), partial

  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein argonaute 10(AGO10) ,partial
  • 貨號：
    CSB-YP893570DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein argonaute 10(AGO10) ,partial
  • 貨號：
    CSB-EP893570DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein argonaute 10(AGO10) ,partial
  • 貨號：
    CSB-EP893570DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein argonaute 10(AGO10) ,partial
  • 貨號：
    CSB-BP893570DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Protein argonaute 10(AGO10) ,partial
  • 貨號：
    CSB-MP893570DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    AGO10
  • Uniprot No.：
  • 別名：
    AGO10; PNH; ZLL; At5g43810; MQD19.17Protein argonaute 10; Protein PINHEAD; Protein ZWILLE
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Required for reliable formation of primary and axillary shoot apical meristems. Specifies leaf adaxial identity by repressing the miR165 and miR166 microRNAs in the embryonic shoot apex, in the shoot apical meristem (SAM) and leaf. Represses the microRNA miR398 which targets CCS1 chaperone mRNAs for translational inhibition. Acts as a negative regulator of AGO1 protein level. Like AGO1, is required for stem cell function and organ polarity. Unlike AGO1, is not subjected to small RNA-mediated repression itself. Essential for multiple processes in development. Coregulates, with GATA18/HAN, the shoot apical meristem (SAM) organization.
  • 基因功能參考文獻：
    1. the shoot regeneration inhibition determined by AGO10 functions via the repression of miR165/166. PMID: 29016889
    2. The regulatory mechanism of AGO10 by which shoot apical meristem size is controlled under stress conditions.[LBD12-1] PMID: 27895202
    3. data suggest that boundary-expressing HAN communicates with the meristem through the PNH, regulates floral organ development via JAG and BOP2, and maintains boundary morphology through CKX3 during flower development in Arabidopsis PMID: 26390296
    4. Our results indicate that maintenance of shoot meristem stem cells by AGO10 involves negative regulation of auxin signaling and, via REV-mediated downregulation of ARF2 expression, auxin response. PMID: 26358077
    5. AGO1, AGO2 and AGO10 promoted anti-TuMV defense in a modular way in various organs, with AGO2 providing a prominent antiviral role in leaves. AGO5, AGO7 and AGO10 had minor effects in leaves. PMID: 25806948
    6. AGO10 quenches miR165/166 that move into AGO10-expressing niches and promotes shoot apical meristem maintenance. PMID: 25801022
    7. AGO10 specifies leaf adaxial identity by repressing miR165/166. PMID: 19054365
    8. ubiquitously expressed ARGONAUTE1 (AGO1) gene and its homolog AGO10, which exhibits highly restricted spatial expression patterns, are required to maintain the correct temporal program of floral stem cells PMID: 21483759
    9. Using an unbiased biochemical approach, we show that AGO10 specifically binds to miR166/165. This association is determined by distinct structural features of the miR166/165 duplex. PMID: 21496644
    10. proposed that ZWILLE buffers meristem stability non-cell-autonomously by ensuring the critical number of apical cells required for proper meristem function PMID: 14564520
    11. Analysis of ago1 mutants with partially compromised AGO1 activity revealed that loss of ZLL function re-establishes both siRNA and miRNA pathways for a subset of AGO1 target genes. PMID: 19763164

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Argonaute family, Ago subfamily
  • 組織特異性：
    Expressed in roots, stems, leaves, developing embryo, siliques, inflorescences, provascular tissue, shoot apical meristem (SAM) and adaxial (upper) sides of lateral organ primordia. Observed in the floral meristem, the adaxial side of sepal primordia, and
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT5G43810

    STRING: 3702.AT5G43810.1

    UniGene: At.11438