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  4. Recombinant Arabidopsis thaliana RPM1-interacting protein 4 (RIN4)

Recombinant Arabidopsis thaliana RPM1-interacting protein 4 (RIN4)

  • 中文名稱：
    擬南芥RIN4重組蛋白
  • 貨號(hào)：
    CSB-YP818127DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥RIN4重組蛋白
  • 貨號(hào)：
    CSB-EP818127DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥RIN4重組蛋白
  • 貨號(hào)：
    CSB-EP818127DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥RIN4重組蛋白
  • 貨號(hào)：
    CSB-BP818127DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥RIN4重組蛋白
  • 貨號(hào)：
    CSB-MP818127DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RIN4
  • Uniprot No.：
  • 別名：
    RIN4; At3g25070; MJL12_1; RPM1-interacting protein 4
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-211
  • 氨基酸序列
    MARSNVPKFG NWEAEENVPY TAYFDKARKT RAPGSKIMNP NDPEYNSDSQ SQAPPHPPSS RTKPEQVDTV RRSREHMRSR EESELKQFGD AGGSSNEAAN KRQGRASQNN SYDNKSPLHK NSYDGTGKSR PKPTNLRADE SPEKVTVVPK FGDWDENNPS SADGYTHIFN KVREERSSGA NVSGSSRTPT HQSSRNPNNT SSCCCFGFGG K
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Essential regulator of plant defense, which plays a central role in resistance in case of infection by a pathogen. It is a common target for both type III avirulence proteins from P.syringae (AvrB, AvrRpm1 and AvrRpt2) and for the plant Resistance (R) proteins RPM1 and RPS2. In strains carrying the appropriate R gene for avirulence proteins of the pathogen, its association with avirulence proteins triggers a defense system including the hypersensitive response, which limits the spread of disease. In contrast, in plants lacking appropriate R genes, its association with avirulence proteins of the pathogen impairs the defense system and leads to the pathogen multiplication.
  • 基因功能參考文獻(xiàn)：
    1. Bacterial effector AvrB-induced RPM1 interacting protein 4 (RIN4) phosphorylation depends on receptor-like cytoplasmic kinase (RLCK) in Arabidopsis. PMID: 28353399
    2. Transiently expressed RIN4, but not the plasma membrane (PM) protein aquaporin PIP2, recruits EXO70B1 to the plasma membrane (PM). Unlike EXO70B1, RIN4 does not recruit the core exocyst subunit SEC6 to the PM under these conditions. PMID: 28338727
    3. RIN4 is intrinsically disordered and it's AvrRpt2 cleavage sites coincide with Molecular Recognition Features (MoRFs) that typically direct protein-protein interaction within other disordered proteins. PMID: 25039985
    4. Consistent with this, RIN4 Pro149 undergoes cis/trans isomerization in the presence of ROC1. PMID: 25299333
    5. Authors demonstrate that recognition of the flagellin peptide microbe-associated molecular pattern flg22 triggers accumulation of RIN4 phosphorylated at serine 141 (pS141) that mediates derepression of several immune outputs. PMID: 25299334
    6. RIN4 is a multifunctional suppressor of PTI and that a virulence function of AvrRpt2 may include cleaving RIN4 into active defense-suppressing fragments. PMID: 21984695
    7. Pseudomonas syringae virulence activity was not observed in plants genetically lacking RIN4. PMID: 20133879
    8. These results indicate that the virulence activity of AvrRpt2 in Arabidopsis thaliana is likely to rely on the modification of host susceptibility factors other than, or in addition to, RIN4. PMID: 15546361
    9. In addition to characterizing the RPS2-RIN4 association in N. benthamiana, we have moved forward to show that the biological relevance of these amino acid changes is applicable in Arabidopsis as well. PMID: 15749765
    10. RIN4 was attached to the plasma membrane at its carboxyl terminus; RIN4 was cleaved by AvrRpt2 at two PxFGxW motifs. PMID: 16478045
    11. Multiple bacterial effectors besides AvrRpt2 elicit proteolysis of RIN4 in planta. PMID: 19671880

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  • 亞細(xì)胞定位：
    Endomembrane system; Peripheral membrane protein.
  • 蛋白家族：
    RIN4 family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT3G25070

    STRING: 3702.AT3G25070.1

    UniGene: At.43082