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  4. Recombinant Arabidopsis thaliana Signal recognition particle 43 kDa protein, chloroplastic (CAO)

Recombinant Arabidopsis thaliana Signal recognition particle 43 kDa protein, chloroplastic (CAO)

  • 中文名稱：
    Recombinant Arabidopsis thaliana Signal recognition particle 43 kDa protein, chloroplastic(CAO)
  • 貨號：
    CSB-YP517766DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Signal recognition particle 43 kDa protein, chloroplastic(CAO)
  • 貨號：
    CSB-EP517766DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Signal recognition particle 43 kDa protein, chloroplastic(CAO)
  • 貨號：
    CSB-EP517766DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Signal recognition particle 43 kDa protein, chloroplastic(CAO)
  • 貨號：
    CSB-BP517766DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Signal recognition particle 43 kDa protein, chloroplastic(CAO)
  • 貨號：
    CSB-MP517766DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CAO
  • Uniprot No.：
  • 別名：
    CAO; CPSRP43; At2g47450; T30B22.25Signal recognition particle 43 kDa protein; chloroplastic; Chromo protein SRP43; CpSRP43
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區(qū)域：
    57-373
  • 氨基酸序列
    AAVQ RNYEETTSSV EEAEEDDESS SSYGEVNKII GSRTAGEGAM EYLIEWKDGH SPSWVPSSYI AADVVSEYET PWWTAARKAD EQALSQLLED RDVDAVDENG RTALLFVAGL GSDKCVRLLA EAGADLDHRD MRGGLTALHM AAGYVRPEVV EALVELGADI EVEDERGLTA LELAREILKT TPKGNPMQFG RRIGLEKVIN VLEGQVFEYA EVDEIVEKRG KGKDVEYLVR WKDGGDCEWV KGVHVAEDVA KDYEDGLEYA VAESVIGKRV GDDGKTIEYL VKWTDMSDAT WEPQDNVDST LVLLYQQQQP MNE
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Component of the chloroplast signal recognition particle pathway. Required for post-translational targeting of proteins into the thylakoid membrane but seems to be dispensable for co-translational targeting with a translating ribosome present. May be able to function independently of cpFTSY and FFC/cpSRP54 in targeting LHCPs to the thylakoids. Acts as a highly specific chaperone for LHCPs, preventing aggregation and being able to dissolve aggregates.
  • 基因功能參考文獻：
    1. CpSRP54 drives cpSRP43 to an active state, binding substrate in the H2O compartment. Bidentate interactions with Alb3 drive it to partial inactivity, selectively releasing LHCP transmembrane domains in a productive unloading complex at the membrane. PMID: 26951662
    2. cpSRP43 chromodomain 3 interacts with a linear motif in the Alb3 C-terminal tail. Study describes the structural basis for selectivity of CPSRP43 chromodomains 2 and 3 for their respective ligands cpSRP54 and Alb3. PMID: 26568381
    3. results support the conclusion that substrate binding to the chloroplast SRP is modulated by protein structural dynamics in which a major role of cpSRP54 is to improve substrate binding efficiency to the cpSRP. PMID: 25918165
    4. The action of cpSRP43 can be dissected into two steps, initial recognition, during which cpSRP43 binds specifically to a recognition motif on the surface of the aggregate, followed by aggregate remodeling. PMID: 23519468
    5. A mechanism for recognition of the LHC aggregate by cpSRP43 elucidated by a biophysical and biochemical approach. PMID: 23525109
    6. cpSRP43 inserts into a dimeric Alb3 translocation pore during cpSRP-dependent delivery of light-harvesting chlorophyll a,b-binding proteins. [SRP43] PMID: 21832051
    7. The authors report the crystal structure of the chloroplast signal recognition particle (cpSRP) core from Arabidopsis thaliana, with the cpSRP54 tail comprising an arginine-rich motif bound to the second chromodomain of cpSRP43. PMID: 22231402
    8. study presents 1.5 angstrom crystal structure of cpSRP43 characterized by a unique arrangement of chromodomains & ankyrin repeats; complex with the internal signal sequence of LHCPs reveals that cpSRP43 specifically recognizes a DPLG peptide motif PMID: 18621669

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  • 亞細胞定位：
    Plastid, chloroplast stroma.
  • 組織特異性：
    Expressed in leaves. Detected in roots.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT2G47450

    STRING: 3702.AT2G47450.1

    UniGene: At.19748