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  4. Recombinant Arabidopsis thaliana Thiamine thiazole synthase, chloroplastic (THI1)

Recombinant Arabidopsis thaliana Thiamine thiazole synthase, chloroplastic (THI1)

  • 中文名稱：
    擬南芥THI1重組蛋白
  • 貨號(hào)：
    CSB-YP656429DOA
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥THI1重組蛋白
  • 貨號(hào)：
    CSB-EP656429DOA
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥THI1重組蛋白
  • 貨號(hào)：
    CSB-EP656429DOA-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥THI1重組蛋白
  • 貨號(hào)：
    CSB-BP656429DOA
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥THI1重組蛋白
  • 貨號(hào)：
    CSB-MP656429DOA
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    THI1
  • Uniprot No.：
  • 別名：
    THI1; ARA6; THI4; At5g54770; MBG8.3; Thiamine thiazole synthase; chloroplastic; EC 2.4.2.60; Thiazole biosynthetic enzyme
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    56-349
  • 氨基酸序列
    FTFDP IKESIVSREM TRRYMTDMIT YAETDVVVVG AGSAGLSAAY EISKNPNVQV AIIEQSVSPG GGAWLGGQLF SAMIVRKPAH LFLDEIGVAY DEQDTYVVVK HAALFTSTIM SKLLARPNVK LFNAVAAEDL IVKGNRVGGV VTNWALVAQN HHTQSCMDPN VMEAKIVVSS CGHDGPFGAT GVKRLKSIGM IDHVPGMKAL DMNTAEDAIV RLTREVVPGM IVTGMEVAEI DGAPRMGPTF GAMMISGQKA GQLALKALGL PNAIDGTLVG NLSPELVLAA ADSAETVDA
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance. Acts as a positive regulator for the abscisic acid-induced activation of slow type anion channels during stomatal closure by repressing CPK33 kinase activity.
  • 基因功能參考文獻(xiàn)：
    1. Consistent with their contrasting regulatory role over stomatal closure, THI1 suppressed CPK33 kinase activity in vitro. PMID: 26662273
    2. The THI1 alanine to valine substitution directly affects the rate of the thiazole biosynthesis. PMID: 24637331
    3. THI1 is targeted to both chloroplasts and mitochondria and is expressed in both shoots and roots during vegetative growth but more active in shoot tissues of mature plants. Expression is affected by high salinity, sugar deprivation and flooding. PMID: 15897230
    4. A point mutation in the thi1 gene, involved in the synthesis of thiamin is responsible for the tz phenotype where the cotyledon is green but the leaves are white, and the plants die early during development. PMID: 12941878
    5. analysis of the thiazole biosynthetic enzyme THI1 from Arabidopsis thaliana PMID: 16912043

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  • 亞細(xì)胞定位：
    Plastid, chloroplast. Mitochondrion. Cell membrane.
  • 蛋白家族：
    THI4 family
  • 組織特異性：
    Expressed at high levels in chloroplast-containing parenchymatic cells of leaves, inflorescence shoots and flowers, and at lower levels in the vascular system. In young plants, detected in roots and shoots including cotyledons, leaves and hypocotyls. Also
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT5G54770

    STRING: 3702.AT5G54770.1

    UniGene: At.24888