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  4. Recombinant Arabidopsis thaliana Violaxanthin de-epoxidase, chloroplastic (VDE1)

Recombinant Arabidopsis thaliana Violaxanthin de-epoxidase, chloroplastic (VDE1)

  • 中文名稱：
    擬南芥VDE1重組蛋白
  • 貨號(hào)：
    CSB-YP655109DOA
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥VDE1重組蛋白
  • 貨號(hào)：
    CSB-EP655109DOA
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥VDE1重組蛋白
  • 貨號(hào)：
    CSB-EP655109DOA-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥VDE1重組蛋白
  • 貨號(hào)：
    CSB-BP655109DOA
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥VDE1重組蛋白
  • 貨號(hào)：
    CSB-MP655109DOA
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    VDE1
  • Uniprot No.：
  • 別名：
    VDE1; AVDE1; NPQ1; VXDE; At1g08550; F22O13.3; T27G7.23; Violaxanthin de-epoxidase; chloroplastic; AtVxDE; EC 1.23.5.1; Protein NON-PHOTOCHEMICAL QUENCHING 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    114-462
  • 氨基酸序列
    VDALKTC ACLLKGCRIE LAKCIANPAC AANVACLQTC NNRPDETECQ IKCGDLFENS VVDEFNECAV SRKKCVPRKS DLGEFPAPDP SVLVQNFNIS DFNGKWYITS GLNPTFDAFD CQLHEFHTEG DNKLVGNISW RIKTLDSGFF TRSAVQKFVQ DPNQPGVLYN HDNEYLHYQD DWYILSSKIE NKPEDYIFVY YRGRNDAWDG YGGAVVYTRS SVLPNSIIPE LEKAAKSIGR DFSTFIRTDN TCGPEPALVE RIEKTVEEGE RIIVKEVEEI EEEVEKEVEK VGRTEMTLFQ RLAEGFNELK QDEENFVREL SKEEMEFLDE IKMEASEVEK LFGKALPIRK VR
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Part of the xanthophyll (or violaxanthin) cycle for controlling the concentration of zeaxanthin in chloroplasts. Catalyzes the two-step mono de-epoxidation reaction. Stereospecific for all-trans xanthophylls. Zeaxanthin induces the dissipation of excitation energy in the chlorophyll of the light-harvesting protein complex of photosystem II.
  • 基因功能參考文獻(xiàn)：
    1. Under a gradually increasing light intensity, where pNPQ is allowed to develop, it is only the amplitude of pNPQ which is the determining factor for protection. Npq1 and npq4 mutants are equally protected against photodamage, but less protected than WT plants.[Npq1] PMID: 25429003
    2. importance of disulfide bonds for violaxanthin de-epoxidase PMID: 25747136
    3. Four amino acid residues (D98, D117, H168 and D206) are critical for pH-dependent activation of violaxanthin de-epoxidase from Arabidopsis thaliana. PMID: 22558195
    4. The phosphorylation of LHC II proteins in the leaves of npq1-2 and npq4-1 mutants of A. thaliana is reported. PMID: 16049777
    5. The action spectrum of photoinhibition in vivo from wild-type Arabidopsis thaliana L. and from the npq1-2 and npq4-1 mutants defective in non-photochemical quenching (NPQ) of excitations of the Photosytem II antenna, was measured. PMID: 16415063
    6. Data determined the structures of the central lipocalin domain of VDE (VDEcd). At neutral pH, VDEcd is monomeric with its active site occluded within a lipocalin barrel. Upon acidification, the barrel opens up and the enzyme appears as a dimer. PMID: 19638474

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  • 亞細(xì)胞定位：
    Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT1G08550

    STRING: 3702.AT1G08550.1

    UniGene: At.20930