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  4. Recombinant Bacillus subtilis RNA polymerase sigma factor sigV (sigV)

Recombinant Bacillus subtilis RNA polymerase sigma factor sigV (sigV)

  • 中文名稱：
    枯草芽孢桿菌sigV重組蛋白
  • 貨號(hào)：
    CSB-YP520830BRJ
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigV重組蛋白
  • 貨號(hào)：
    CSB-EP520830BRJ
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigV重組蛋白
  • 貨號(hào)：
    CSB-EP520830BRJ-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigV重組蛋白
  • 貨號(hào)：
    CSB-BP520830BRJ
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigV重組蛋白
  • 貨號(hào)：
    CSB-MP520830BRJ
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    sigV
  • Uniprot No.：
  • 別名：
    sigV; BSU27120; RNA polymerase sigma factor SigV
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-166
  • 氨基酸序列
    MKKKQTTKAL LVTCITDHKQ DFYRLAFSYV KNQDDALDIV QESIKKALSS VETVRNPETI KSWFYKILVR TAIDFLRKQK KIRVMDDETI EFLSKGKEDH YKDTDLHEAL DELPYRYKTI IILRFFEDLK LEEIAEITGE NTNTVKTRLY RALKLMRIQL TKEDLS
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Positively regulates the expression of proteins involved in stress responses against bacitracin, paraquat and tellurite.
  • 基因功能參考文獻(xiàn)：
    1. RsiV controls activation of the factor sigma V. PMID: 25275625
    2. Authors demonstrate that the activation of a member of the ECF30 subfamily of ECF sigma factors, sigma(V) in Bacillus subtilis, is controlled by the proteolytic destruction of the anti-sigma factor RsiV. PMID: 23687273
    3. SigV activated two cell wall modification pathways, peptidoglycan O-acetylation and D-alanylation of teichoic acids. PMID: 21926231
    4. Lysozyme induces sigV expression. PMID: 21856855
  • 蛋白家族：
    Sigma-70 factor family, ECF subfamily
  • 數(shù)據(jù)庫(kù)鏈接：