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  4. Recombinant Bacillus subtilis Sporulation-killing factor skfA (skfA)

Recombinant Bacillus subtilis Sporulation-killing factor skfA (skfA)

  • 中文名稱：
    枯草芽孢桿菌skfA重組蛋白
  • 貨號(hào)：
    CSB-YP518230BRJ
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌skfA重組蛋白
  • 貨號(hào)：
    CSB-EP518230BRJ
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌skfA重組蛋白
  • 貨號(hào)：
    CSB-EP518230BRJ-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌skfA重組蛋白
  • 貨號(hào)：
    CSB-BP518230BRJ
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌skfA重組蛋白
  • 貨號(hào)：
    CSB-MP518230BRJ
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    skfA
  • Uniprot No.：
  • 別名：
    skfA; ybcO; BSU01910; Sporulation killing factor; SKF; Sporulation-killing factor SkfA
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-55
  • 氨基酸序列
    MKRNQKEWES VSKKGLMKPG GTSIVKAAGC MGCWASKSIA MTRVCALPHP AMRAI
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Produces a 26-residue extracellular sporulation filling factor (SKF) that induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, providing a source of nutrients to support sporulation, and at the same time delaying commitment to the energetically expensive and irreversible onset of sporulation. Can also inhibit growth of other bacteria at high concentrations. Addition of SKF to solid cultures induces killing, but it is much less effective than SDP (AC O34344). Has a role in protecting the secreted lipase LipA against proteolysis, either by modulating its folding or by acting as a protease inhibitor.
  • 基因功能參考文獻(xiàn)：
    1. The radical SAM enzyme, SkfB, utilizes the 5'-deoxyadenosyl radical generated from the reductive cleavage of SAM to abstract a hydrogen atom from the alpha-carbon of the amino acid at position 12 in the substrate, SkfA, to initiate the installation of a thioether cross-link. PMID: 27410522
  • 亞細(xì)胞定位：
    Secreted.
  • 數(shù)據(jù)庫鏈接：