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  4. Recombinant Bovine Deoxyribonuclease-1 (DNASE1)

Recombinant Bovine Deoxyribonuclease-1 (DNASE1)

  • 中文名稱：
    牛DNASE1重組蛋白
  • 貨號(hào)：
    CSB-YP007049BO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛DNASE1重組蛋白
  • 貨號(hào)：
    CSB-EP007049BO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛DNASE1重組蛋白
  • 貨號(hào)：
    CSB-EP007049BO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛DNASE1重組蛋白
  • 貨號(hào)：
    CSB-BP007049BO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛DNASE1重組蛋白
  • 貨號(hào)：
    CSB-MP007049BO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    DNASE1
  • Uniprot No.：
  • 別名：
    DNASE1; DNL1Deoxyribonuclease-1; EC 3.1.21.1; Deoxyribonuclease I; DNase I
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    23-282
  • 氨基酸序列
    LKIAAFNI RTFGETKMSN ATLASYIVRI VRRYDIVLIQ EVRDSHLVAV GKLLDYLNQD DPNTYHYVVS EPLGRNSYKE RYLFLFRPNK VSVLDTYQYD DGCESCGNDS FSREPAVVKF SSHSTKVKEF AIVALHSAPS DAVAEINSLY DVYLDVQQKW HLNDVMLMGD FNADCSYVTS SQWSSIRLRT SSTFQWLIPD SADTTATSTN CAYDRIVVAG SLLQSSVVPG SAAPFDFQAA YGLSNEMALA ISDHYPVEVT LT
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

相關(guān)產(chǎn)品

靶點(diǎn)詳情

  • 功能：
    Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
  • 基因功能參考文獻(xiàn)：
    1. ascorbic acid has the ability to inhibit DNase I, one of the main endonucleases involved in DNA fragmentation during apoptosis. PMID: 29551685
    2. the practical capability of this assay system was successfully demonstrated by its use to determine DNase I activity in bovine urine. PMID: 27570054
    3. Specificity in DNA-DNase I interaction is mediated by DNA flexibility, which influences the induced-fit transitions required to form productive complexes. PMID: 19850052
    4. results indicated that the backbone hydrogen bonding in the antiparallel beta-sheet formed by its N- and C-termini, rather than the side-chain interactions, is crucial for the correct protein folding PMID: 15311926
  • 亞細(xì)胞定位：
    Secreted. Zymogen granule. Nucleus envelope.
  • 蛋白家族：
    DNase I family
  • 數(shù)據(jù)庫鏈接：