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  4. Bovine Deoxyribonuclease-1(DNASE1) ELISA kit

Bovine Deoxyribonuclease-1(DNASE1) ELISA kit

  • 中文名稱：
    牛脫氧核糖核酸酶1(DNASE1)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL007049BO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    牛脫氧核糖核酸酶1(DNASE1)酶聯(lián)免疫試劑盒（CSB-EL007049BO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DNASE1含量。DNASE1是重要的靶點(diǎn)。它是一種核酸酶，可降解DNA。背景上，其在維持細(xì)胞內(nèi)環(huán)境穩(wěn)定等方面有重要意義。研究機(jī)制方面，主要聚焦其降解DNA的具體過程、調(diào)控因素，以及在相關(guān)疾病如自身免疫病中因功能異常對病情發(fā)展的影響。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，可為科研領(lǐng)域研究牛DNASE1在細(xì)胞凋亡機(jī)制、炎癥反應(yīng)或免疫調(diào)節(jié)中的功能提供可靠工具，尤其適用于畜牧病理學(xué)、動物免疫學(xué)研究中對相關(guān)生物標(biāo)志物的定量分析需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    DNASE1 ELISA Kit; DNL1Deoxyribonuclease-1 ELISA Kit; EC 3.1.21.1 ELISA Kit; Deoxyribonuclease I ELISA Kit; DNase I ELISA Kit
  • 縮寫：
    DNASE1
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    12.5 pg/mL-800 pg/mL
  • 靈敏度：
    3.1 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Epigenetics and Nuclear Signaling
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine DNASE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:20 Average % 85
    Range % 80-89
    1:40 Average % 94
    Range % 90-98
    1:80 Average % 103
    Range % 99-109
    1:160 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of bovine DNASE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 88 84-92
    EDTA plasma (n=4) 93 89-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    800 2.399 2.416 2.408 2.273
    400 2.213 2.195 2.204 2.069
    200 1.813 1.741 1.777 1.642
    100 1.204 1.227 1.216 1.081
    50 0.683 0.713 0.698 0.563
    25 0.400 0.392 0.396 0.261
    12.5 0.292 0.284 0.288 0.153
    0 0.131 0.138 0.135 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
  • 基因功能參考文獻(xiàn)：
    1. ascorbic acid has the ability to inhibit DNase I, one of the main endonucleases involved in DNA fragmentation during apoptosis. PMID: 29551685
    2. the practical capability of this assay system was successfully demonstrated by its use to determine DNase I activity in bovine urine. PMID: 27570054
    3. Specificity in DNA-DNase I interaction is mediated by DNA flexibility, which influences the induced-fit transitions required to form productive complexes. PMID: 19850052
    4. results indicated that the backbone hydrogen bonding in the antiparallel beta-sheet formed by its N- and C-termini, rather than the side-chain interactions, is crucial for the correct protein folding PMID: 15311926
  • 亞細(xì)胞定位：
    Secreted. Zymogen granule. Nucleus envelope.
  • 蛋白家族：
    DNase I family
  • 數(shù)據(jù)庫鏈接：