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  4. Recombinant Drosophila melanogaster Myosin-VIIa (ck), partial

Recombinant Drosophila melanogaster Myosin-VIIa (ck), partial

  • 中文名稱：
    Recombinant Drosophila melanogaster Myosin-VIIa(ck) ,partial
  • 貨號：
    CSB-YP896042DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Myosin-VIIa(ck) ,partial
  • 貨號：
    CSB-EP896042DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Myosin-VIIa(ck) ,partial
  • 貨號：
    CSB-EP896042DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Myosin-VIIa(ck) ,partial
  • 貨號：
    CSB-BP896042DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Drosophila melanogaster Myosin-VIIa(ck) ,partial
  • 貨號：
    CSB-MP896042DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ck
  • Uniprot No.：
  • 別名：
    ck; CG7595; Myosin-VIIa; DmVIIa; Protein crinkled
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements: can function in cells as a single-molecule cargo transporter. A very slow and high-duty-ratio motor, may be suitable for tension maintenance of actin filaments. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Plays a key role in the formation of cellular projections and other actin-based functions required for embryonic and larval viability. Necessary for auditory transduction: plays a role in Johnston's organ organization by functioning in scolopidial apical attachment and therefore to acoustic stimulus propagation from the antenna a2/a3 joint to transducing elements. Interaction with the myosin zip may be important for its function in scolopidial apical attachment. During oogenesis it has Cad99c-dependent and Cad99c-independent roles in regulating the shape and spacing of the follicle cell microvilli which secrete eggshell material such as the vitelline membrane. May be required for the normal expression of Cad99c in the follicle cell microvilli.
  • 基因功能參考文獻：
    1. CK selectively interacts with the initiator caspase DRONC and regulates some of its non-apoptotic functions. Results expose a conserved role for unconventional myosins in transducing caspase-dependent regulation of kinases. PMID: 26960254
    2. Product identified as myosin VIIA. PMID: 15579689
    3. Loss of ck/myoVIIA function leads to complete deafness in Drosophila by disrupting the integrity of the scolopidia that transduce auditory signals. PMID: 15886106
    4. myosin VIIA is classified to be a high duty ratio motor PMID: 16415346
    5. kinetic behavior would allow myosin VIIa to exert and hold tension on actin filaments and, if dimerized, to function as a processive cargo transporter. PMID: 16585515
    6. domain markedly inhibits the actin-activated ATPase activity of tailless DM7A at low Ca(2+) but not high Ca(2+) PMID: 19423668

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  • 亞細胞定位：
    Cytoplasm. Cytoplasm, cell cortex. Cell projection, microvillus.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
  • 組織特異性：
    Expressed in the setae, micro- and macrochaetae on the head, thorax and wing.
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG7595

    STRING: 7227.FBpp0080282

    UniGene: Dm.2128