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  4. Recombinant Drosophila melanogaster Phosphatidylinositol-binding clathrin assembly protein LAP (lap)

Recombinant Drosophila melanogaster Phosphatidylinositol-binding clathrin assembly protein LAP (lap)

  • 中文名稱：
    黑腹果蠅lap重組蛋白
  • 貨號：
    CSB-YP893285DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅lap重組蛋白
  • 貨號：
    CSB-EP893285DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅lap重組蛋白
  • 貨號：
    CSB-EP893285DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅lap重組蛋白
  • 貨號：
    CSB-BP893285DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅lap重組蛋白
  • 貨號：
    CSB-MP893285DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    lap
  • Uniprot No.：
  • 別名：
    lap; CG2520; Phosphatidylinositol-binding clathrin assembly protein LAP; Like-AP180
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-468
  • 氨基酸序列
    MTMAGQTIND RLLAARHSLA GQGLAKSVCK ATTEECIGPK KKHLDYLVHC TNEPNVSIPH LANLLIERSQ NANWVVVYKS LITTHHLMAY GNERFMQYLA SSNSTFNLSS FLDKGTVQDG GMGVPGGRMG YDMSPFIRRY AKYLNEKSLS YRAMAFDFCK VKRGKEEGSL RSMNAEKLLK TLPVLQAQLD ALLEFDCQSN DLSNGVINMS FMLLFRDLIR LFACYNDGII NLLEKYFDMN KKHARDALDL YKKFLVRMDR VGEFLKVAEN VGIDKGDIPD LTKAPSSLLD ALEQHLATLE GRKVSAANTP TQSSSSAFGT AAASSKFDTT NGIDEQLKAQ VLAEEEAAMN QYKSKVSSPT SSGAAGASAA LTNPFLSSPP AAQAGQPIVD LFGAASAQPA AAAAATKASD DLLQLGNPFA DMFDASGGGA AAVGATGNAG DGTAKYDGGA GSSPFDWGAT DDDGGAAQ
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.
  • 基因功能參考文獻：
    1. results reveal a new mode by which AP180 couples protein retrieval to CME of SVs PMID: 24456281
    2. clathrin adapter protein PMID: 20391775
  • 亞細胞定位：
    Membrane, clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle. Note=Colocalized with clathrin in the Golgi area.
  • 蛋白家族：
    PICALM/SNAP91 family
  • 組織特異性：
    In embryos, expression is seen in central and peripheral nervous systems (brain and ventral nerve cord) and Garland cells. Coexpressed with clathrin at presynaptic boutons of neuromuscular junctions.
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG2520

    STRING: 7227.FBpp0291394

    UniGene: Dm.2478